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A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption f...

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Detalles Bibliográficos
Autor principal: Patel, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6463175/
https://www.ncbi.nlm.nih.gov/pubmed/30609833
http://dx.doi.org/10.3390/jof5010004
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author Patel, Robin
author_facet Patel, Robin
author_sort Patel, Robin
collection PubMed
description As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.
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spelling pubmed-64631752019-04-17 A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification Patel, Robin J Fungi (Basel) Review As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed. MDPI 2019-01-03 /pmc/articles/PMC6463175/ /pubmed/30609833 http://dx.doi.org/10.3390/jof5010004 Text en © 2019 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Patel, Robin
A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_full A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_fullStr A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_full_unstemmed A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_short A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_sort moldy application of maldi: maldi-tof mass spectrometry for fungal identification
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6463175/
https://www.ncbi.nlm.nih.gov/pubmed/30609833
http://dx.doi.org/10.3390/jof5010004
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