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124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics
[Image: see text] Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiple...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6463241/ https://www.ncbi.nlm.nih.gov/pubmed/30864449 http://dx.doi.org/10.1021/acs.nanolett.9b00508 |
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author | Wade, Orsolya K. Woehrstein, Johannes B. Nickels, Philipp C. Strauss, Sebastian Stehr, Florian Stein, Johannes Schueder, Florian Strauss, Maximilian T. Ganji, Mahipal Schnitzbauer, Joerg Grabmayr, Heinrich Yin, Peng Schwille, Petra Jungmann, Ralf |
author_facet | Wade, Orsolya K. Woehrstein, Johannes B. Nickels, Philipp C. Strauss, Sebastian Stehr, Florian Stein, Johannes Schueder, Florian Strauss, Maximilian T. Ganji, Mahipal Schnitzbauer, Joerg Grabmayr, Heinrich Yin, Peng Schwille, Petra Jungmann, Ralf |
author_sort | Wade, Orsolya K. |
collection | PubMed |
description | [Image: see text] Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes. |
format | Online Article Text |
id | pubmed-6463241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-64632412019-04-16 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics Wade, Orsolya K. Woehrstein, Johannes B. Nickels, Philipp C. Strauss, Sebastian Stehr, Florian Stein, Johannes Schueder, Florian Strauss, Maximilian T. Ganji, Mahipal Schnitzbauer, Joerg Grabmayr, Heinrich Yin, Peng Schwille, Petra Jungmann, Ralf Nano Lett [Image: see text] Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes. American Chemical Society 2019-03-13 2019-04-10 /pmc/articles/PMC6463241/ /pubmed/30864449 http://dx.doi.org/10.1021/acs.nanolett.9b00508 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Wade, Orsolya K. Woehrstein, Johannes B. Nickels, Philipp C. Strauss, Sebastian Stehr, Florian Stein, Johannes Schueder, Florian Strauss, Maximilian T. Ganji, Mahipal Schnitzbauer, Joerg Grabmayr, Heinrich Yin, Peng Schwille, Petra Jungmann, Ralf 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics |
title | 124-Color Super-resolution Imaging by Engineering
DNA-PAINT Blinking Kinetics |
title_full | 124-Color Super-resolution Imaging by Engineering
DNA-PAINT Blinking Kinetics |
title_fullStr | 124-Color Super-resolution Imaging by Engineering
DNA-PAINT Blinking Kinetics |
title_full_unstemmed | 124-Color Super-resolution Imaging by Engineering
DNA-PAINT Blinking Kinetics |
title_short | 124-Color Super-resolution Imaging by Engineering
DNA-PAINT Blinking Kinetics |
title_sort | 124-color super-resolution imaging by engineering
dna-paint blinking kinetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6463241/ https://www.ncbi.nlm.nih.gov/pubmed/30864449 http://dx.doi.org/10.1021/acs.nanolett.9b00508 |
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