Cargando…
Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea
Sound analysis by the cochlea relies on frequency tuning of mechanosensory hair cells along a tonotopic axis. To clarify the underlying biophysical mechanism, we have investigated the micromechanical properties of the hair cell’s mechanoreceptive hair bundle within the apical half of the rat cochlea...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6464607/ https://www.ncbi.nlm.nih.gov/pubmed/30932811 http://dx.doi.org/10.7554/eLife.43473 |
_version_ | 1783410875492728832 |
---|---|
author | Tobin, Mélanie Chaiyasitdhi, Atitheb Michel, Vincent Michalski, Nicolas Martin, Pascal |
author_facet | Tobin, Mélanie Chaiyasitdhi, Atitheb Michel, Vincent Michalski, Nicolas Martin, Pascal |
author_sort | Tobin, Mélanie |
collection | PubMed |
description | Sound analysis by the cochlea relies on frequency tuning of mechanosensory hair cells along a tonotopic axis. To clarify the underlying biophysical mechanism, we have investigated the micromechanical properties of the hair cell’s mechanoreceptive hair bundle within the apical half of the rat cochlea. We studied both inner and outer hair cells, which send nervous signals to the brain and amplify cochlear vibrations, respectively. We find that tonotopy is associated with gradients of stiffness and resting mechanical tension, with steeper gradients for outer hair cells, emphasizing the division of labor between the two hair-cell types. We demonstrate that tension in the tip links that convey force to the mechano-electrical transduction channels increases at reduced Ca(2+). Finally, we reveal gradients in stiffness and tension at the level of a single tip link. We conclude that mechanical gradients of the tip-link complex may help specify the characteristic frequency of the hair cell. |
format | Online Article Text |
id | pubmed-6464607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-64646072019-04-17 Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea Tobin, Mélanie Chaiyasitdhi, Atitheb Michel, Vincent Michalski, Nicolas Martin, Pascal eLife Neuroscience Sound analysis by the cochlea relies on frequency tuning of mechanosensory hair cells along a tonotopic axis. To clarify the underlying biophysical mechanism, we have investigated the micromechanical properties of the hair cell’s mechanoreceptive hair bundle within the apical half of the rat cochlea. We studied both inner and outer hair cells, which send nervous signals to the brain and amplify cochlear vibrations, respectively. We find that tonotopy is associated with gradients of stiffness and resting mechanical tension, with steeper gradients for outer hair cells, emphasizing the division of labor between the two hair-cell types. We demonstrate that tension in the tip links that convey force to the mechano-electrical transduction channels increases at reduced Ca(2+). Finally, we reveal gradients in stiffness and tension at the level of a single tip link. We conclude that mechanical gradients of the tip-link complex may help specify the characteristic frequency of the hair cell. eLife Sciences Publications, Ltd 2019-04-01 /pmc/articles/PMC6464607/ /pubmed/30932811 http://dx.doi.org/10.7554/eLife.43473 Text en © 2019, Tobin et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Tobin, Mélanie Chaiyasitdhi, Atitheb Michel, Vincent Michalski, Nicolas Martin, Pascal Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea |
title | Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea |
title_full | Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea |
title_fullStr | Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea |
title_full_unstemmed | Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea |
title_short | Stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea |
title_sort | stiffness and tension gradients of the hair cell’s tip-link complex in the mammalian cochlea |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6464607/ https://www.ncbi.nlm.nih.gov/pubmed/30932811 http://dx.doi.org/10.7554/eLife.43473 |
work_keys_str_mv | AT tobinmelanie stiffnessandtensiongradientsofthehaircellstiplinkcomplexinthemammaliancochlea AT chaiyasitdhiatitheb stiffnessandtensiongradientsofthehaircellstiplinkcomplexinthemammaliancochlea AT michelvincent stiffnessandtensiongradientsofthehaircellstiplinkcomplexinthemammaliancochlea AT michalskinicolas stiffnessandtensiongradientsofthehaircellstiplinkcomplexinthemammaliancochlea AT martinpascal stiffnessandtensiongradientsofthehaircellstiplinkcomplexinthemammaliancochlea |