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A combined in vitro / in vivo selection for polymerases with novel promoter specificities

BACKGROUND: The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its promoter. A combined in vitro / in vivo selection method has been developed for the evolution of T7 RNA polymerases with...

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Detalles Bibliográficos
Autores principales:	Chelliserrykattil, Jijumon, Cai, George, Ellington, Andrew D
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2001
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC64648/ https://www.ncbi.nlm.nih.gov/pubmed/11806761 http://dx.doi.org/10.1186/1472-6750-1-13

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