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A combined in vitro / in vivo selection for polymerases with novel promoter specificities
BACKGROUND: The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its promoter. A combined in vitro / in vivo selection method has been developed for the evolution of T7 RNA polymerases with...
Autores principales: | Chelliserrykattil, Jijumon, Cai, George, Ellington, Andrew D |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC64648/ https://www.ncbi.nlm.nih.gov/pubmed/11806761 http://dx.doi.org/10.1186/1472-6750-1-13 |
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