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Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure
Ligustici Rhizoma et Radix (LReR), an important Chinese medicine known as “Gao-ben,” refers to Ligusticum sinense Oliv. or Ligusticum jeholense Nakai et Kitag. However, a number of other species are commonly sold as “Gao-ben” in the herbal medicine market, which may result in a series of quality con...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465525/ https://www.ncbi.nlm.nih.gov/pubmed/31024595 http://dx.doi.org/10.3389/fpls.2019.00429 |
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author | Liu, Zhen-wen Gao, Yu-zhen Zhou, Jing |
author_facet | Liu, Zhen-wen Gao, Yu-zhen Zhou, Jing |
author_sort | Liu, Zhen-wen |
collection | PubMed |
description | Ligustici Rhizoma et Radix (LReR), an important Chinese medicine known as “Gao-ben,” refers to Ligusticum sinense Oliv. or Ligusticum jeholense Nakai et Kitag. However, a number of other species are commonly sold as “Gao-ben” in the herbal medicine market, which may result in a series of quality control problems and inconsistent therapeutic effects. The “Gao-ben” is commonly sold sliced and dried, making traditional identification methods difficult. Here, the mini barcode ITS2 region was examined on 68 samples representing LReR and 7 potential adulterant or substitute species. The results showed 100% success rates of PCR and sequencing and the existence of a barcoding gap. The neighbor-joining (NJ) tree indicated that all the tested samples could be exactly identified. The ITS2 secondary structure revealed a clear difference between true “Gao-ben” and three adulterant species. We therefore recommend the use of ITS2 as a mini barcode for distinguishing between closely or distantly related plant species that may be used in Chinese medicine. |
format | Online Article Text |
id | pubmed-6465525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64655252019-04-25 Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure Liu, Zhen-wen Gao, Yu-zhen Zhou, Jing Front Plant Sci Plant Science Ligustici Rhizoma et Radix (LReR), an important Chinese medicine known as “Gao-ben,” refers to Ligusticum sinense Oliv. or Ligusticum jeholense Nakai et Kitag. However, a number of other species are commonly sold as “Gao-ben” in the herbal medicine market, which may result in a series of quality control problems and inconsistent therapeutic effects. The “Gao-ben” is commonly sold sliced and dried, making traditional identification methods difficult. Here, the mini barcode ITS2 region was examined on 68 samples representing LReR and 7 potential adulterant or substitute species. The results showed 100% success rates of PCR and sequencing and the existence of a barcoding gap. The neighbor-joining (NJ) tree indicated that all the tested samples could be exactly identified. The ITS2 secondary structure revealed a clear difference between true “Gao-ben” and three adulterant species. We therefore recommend the use of ITS2 as a mini barcode for distinguishing between closely or distantly related plant species that may be used in Chinese medicine. Frontiers Media S.A. 2019-04-09 /pmc/articles/PMC6465525/ /pubmed/31024595 http://dx.doi.org/10.3389/fpls.2019.00429 Text en Copyright © 2019 Liu, Gao and Zhou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Liu, Zhen-wen Gao, Yu-zhen Zhou, Jing Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure |
title | Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure |
title_full | Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure |
title_fullStr | Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure |
title_full_unstemmed | Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure |
title_short | Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure |
title_sort | molecular authentication of the medicinal species of ligusticum (ligustici rhizoma et radix, “gao-ben”) by integrating non-coding internal transcribed spacer 2 (its2) and its secondary structure |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465525/ https://www.ncbi.nlm.nih.gov/pubmed/31024595 http://dx.doi.org/10.3389/fpls.2019.00429 |
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