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Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection

Backgrounds and Aims: We previously demonstrated that serum hepatitis B virus (HBV) DNA in HBV infected humanized mice exhibited a highly dynamic multiphasic kinetic pattern from infection initiation to steady-state. Here, we investigated whether this pattern is consistent across different HBV clone...

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Autores principales: Tsuge, Masataka, Uchida, Takuro, Walsh, Kevin, Ishida, Yuji, Tateno, Chise, Kumar, Upendra, Glenn, Jeffrey S., Koh, Christopher, Heller, Theo, Uprichard, Susan L., Dahari, Harel, Chayama, Kazuaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466026/
https://www.ncbi.nlm.nih.gov/pubmed/30875937
http://dx.doi.org/10.3390/v11030263
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author Tsuge, Masataka
Uchida, Takuro
Walsh, Kevin
Ishida, Yuji
Tateno, Chise
Kumar, Upendra
Glenn, Jeffrey S.
Koh, Christopher
Heller, Theo
Uprichard, Susan L.
Dahari, Harel
Chayama, Kazuaki
author_facet Tsuge, Masataka
Uchida, Takuro
Walsh, Kevin
Ishida, Yuji
Tateno, Chise
Kumar, Upendra
Glenn, Jeffrey S.
Koh, Christopher
Heller, Theo
Uprichard, Susan L.
Dahari, Harel
Chayama, Kazuaki
author_sort Tsuge, Masataka
collection PubMed
description Backgrounds and Aims: We previously demonstrated that serum hepatitis B virus (HBV) DNA in HBV infected humanized mice exhibited a highly dynamic multiphasic kinetic pattern from infection initiation to steady-state. Here, we investigated whether this pattern is consistent across different HBV clones or in the presence of hepatitis D virus (HDV) co-infection. Methods: We analyzed early serum viral kinetics using 26 HBV genotype C (GtC) mono-infected mice [clones: PXB, Hiroshima GtC CL4 (CL4) and Hiroshima GtC CL5 (CL5)] and four HBV CL4/HDV genotype one co-infected mice. Results: The HBV kinetics observed with clones CL4 and CL5 were similar to that previously defined in HBV PXB infected mice. Additionally, no significant differences in HBV DNA levels were observed between HBV mono-infected and HBV/HDV co-infected mice through 4 weeks post-inoculation (p.i.). However, HBV DNA levels at 6 weeks p.i. in HBV/HDV co-infected mice were significantly lower than those in HBV mono-infected mice (P = 0.002), consistent with HDV suppression of chronic HBV. Conclusions: HBV infection initiation is multiphasic across multiple viral clones and is not altered by HDV co-infection. The latter suggests that higher HDV titers (>8 log IU/mL) and/or longer duration of HDV infection might be needed to trigger HDV-induced suppression on HBV.
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spelling pubmed-64660262019-04-18 Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection Tsuge, Masataka Uchida, Takuro Walsh, Kevin Ishida, Yuji Tateno, Chise Kumar, Upendra Glenn, Jeffrey S. Koh, Christopher Heller, Theo Uprichard, Susan L. Dahari, Harel Chayama, Kazuaki Viruses Communication Backgrounds and Aims: We previously demonstrated that serum hepatitis B virus (HBV) DNA in HBV infected humanized mice exhibited a highly dynamic multiphasic kinetic pattern from infection initiation to steady-state. Here, we investigated whether this pattern is consistent across different HBV clones or in the presence of hepatitis D virus (HDV) co-infection. Methods: We analyzed early serum viral kinetics using 26 HBV genotype C (GtC) mono-infected mice [clones: PXB, Hiroshima GtC CL4 (CL4) and Hiroshima GtC CL5 (CL5)] and four HBV CL4/HDV genotype one co-infected mice. Results: The HBV kinetics observed with clones CL4 and CL5 were similar to that previously defined in HBV PXB infected mice. Additionally, no significant differences in HBV DNA levels were observed between HBV mono-infected and HBV/HDV co-infected mice through 4 weeks post-inoculation (p.i.). However, HBV DNA levels at 6 weeks p.i. in HBV/HDV co-infected mice were significantly lower than those in HBV mono-infected mice (P = 0.002), consistent with HDV suppression of chronic HBV. Conclusions: HBV infection initiation is multiphasic across multiple viral clones and is not altered by HDV co-infection. The latter suggests that higher HDV titers (>8 log IU/mL) and/or longer duration of HDV infection might be needed to trigger HDV-induced suppression on HBV. MDPI 2019-03-15 /pmc/articles/PMC6466026/ /pubmed/30875937 http://dx.doi.org/10.3390/v11030263 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Tsuge, Masataka
Uchida, Takuro
Walsh, Kevin
Ishida, Yuji
Tateno, Chise
Kumar, Upendra
Glenn, Jeffrey S.
Koh, Christopher
Heller, Theo
Uprichard, Susan L.
Dahari, Harel
Chayama, Kazuaki
Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection
title Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection
title_full Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection
title_fullStr Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection
title_full_unstemmed Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection
title_short Early Multiphasic HBV Infection Initiation Kinetics Is Not Clone-Specific and Is Not Affected by Hepatitis D Virus (HDV) Infection
title_sort early multiphasic hbv infection initiation kinetics is not clone-specific and is not affected by hepatitis d virus (hdv) infection
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466026/
https://www.ncbi.nlm.nih.gov/pubmed/30875937
http://dx.doi.org/10.3390/v11030263
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