Cargando…

Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis

Canine mammary tumors (CMT) represent the most common cancer in noncastrated female dogs. Interestingly, triple-negative tumors are the most common molecular subtype in female dogs. In this study, we proposed to evaluate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2), Plat...

Descripción completa

Detalles Bibliográficos
Autores principales: dos Anjos, Denner Santos, Vital, Aline Fernandes, Lainetti, Patrícia de Faria, Leis-Filho, Antonio Fernando, Dalmolin, Fabiola, Elias, Fabiana, Calazans, Sabryna Gouveia, Fonseca-Alves, Carlos Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466043/
https://www.ncbi.nlm.nih.gov/pubmed/30634402
http://dx.doi.org/10.3390/vetsci6010003
_version_ 1783411019179098112
author dos Anjos, Denner Santos
Vital, Aline Fernandes
Lainetti, Patrícia de Faria
Leis-Filho, Antonio Fernando
Dalmolin, Fabiola
Elias, Fabiana
Calazans, Sabryna Gouveia
Fonseca-Alves, Carlos Eduardo
author_facet dos Anjos, Denner Santos
Vital, Aline Fernandes
Lainetti, Patrícia de Faria
Leis-Filho, Antonio Fernando
Dalmolin, Fabiola
Elias, Fabiana
Calazans, Sabryna Gouveia
Fonseca-Alves, Carlos Eduardo
author_sort dos Anjos, Denner Santos
collection PubMed
description Canine mammary tumors (CMT) represent the most common cancer in noncastrated female dogs. Interestingly, triple-negative tumors are the most common molecular subtype in female dogs. In this study, we proposed to evaluate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2), Platelet-derived growth factor receptor (PDGFR), and microvascular density (MVD) in a group of metastatic and nonmetastatic triple-negative CMT and compare the expression based on clinical parameters. Twenty-six female dogs with triple-negative mammary tumors were divided into three groups: nonmetastatic tumors (NMT) (n = 11), tumors with lymph node metastasis (LNM) (n = 10), and tumors with lung metastasis (LM) (n = 5). We observed increased VEGFR-2 expression in LNM compared with NMT and a positive correlation between tumor grade and VEGFR-2 expression. A positive correlation was noted between VEGFR-2 and PDGFR expression. Regarding microvascular density (MVD), we identified a higher number of vessels in primary tumors with lymph node metastasis and lung metastasis compared with tumors with no metastasis. The primary tumors with lung metastasis exhibited an increased MVD compared with carcinoma with lymph node metastasis. Overall, our results suggest a deregulation of VEGFR-2 and PDGFR and high MVD in metastatic tumors, indicating a role for angiogenesis in tumor progression.
format Online
Article
Text
id pubmed-6466043
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64660432019-04-19 Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis dos Anjos, Denner Santos Vital, Aline Fernandes Lainetti, Patrícia de Faria Leis-Filho, Antonio Fernando Dalmolin, Fabiola Elias, Fabiana Calazans, Sabryna Gouveia Fonseca-Alves, Carlos Eduardo Vet Sci Article Canine mammary tumors (CMT) represent the most common cancer in noncastrated female dogs. Interestingly, triple-negative tumors are the most common molecular subtype in female dogs. In this study, we proposed to evaluate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2), Platelet-derived growth factor receptor (PDGFR), and microvascular density (MVD) in a group of metastatic and nonmetastatic triple-negative CMT and compare the expression based on clinical parameters. Twenty-six female dogs with triple-negative mammary tumors were divided into three groups: nonmetastatic tumors (NMT) (n = 11), tumors with lymph node metastasis (LNM) (n = 10), and tumors with lung metastasis (LM) (n = 5). We observed increased VEGFR-2 expression in LNM compared with NMT and a positive correlation between tumor grade and VEGFR-2 expression. A positive correlation was noted between VEGFR-2 and PDGFR expression. Regarding microvascular density (MVD), we identified a higher number of vessels in primary tumors with lymph node metastasis and lung metastasis compared with tumors with no metastasis. The primary tumors with lung metastasis exhibited an increased MVD compared with carcinoma with lymph node metastasis. Overall, our results suggest a deregulation of VEGFR-2 and PDGFR and high MVD in metastatic tumors, indicating a role for angiogenesis in tumor progression. MDPI 2019-01-09 /pmc/articles/PMC6466043/ /pubmed/30634402 http://dx.doi.org/10.3390/vetsci6010003 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
dos Anjos, Denner Santos
Vital, Aline Fernandes
Lainetti, Patrícia de Faria
Leis-Filho, Antonio Fernando
Dalmolin, Fabiola
Elias, Fabiana
Calazans, Sabryna Gouveia
Fonseca-Alves, Carlos Eduardo
Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis
title Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis
title_full Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis
title_fullStr Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis
title_full_unstemmed Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis
title_short Deregulation of VEGFR-2 and PDGFR Expression and Microvascular Density in a Triple-Negative Model of Canine Malignant Mammary Tumors with Lymph Node or Lung Metastasis
title_sort deregulation of vegfr-2 and pdgfr expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466043/
https://www.ncbi.nlm.nih.gov/pubmed/30634402
http://dx.doi.org/10.3390/vetsci6010003
work_keys_str_mv AT dosanjosdennersantos deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis
AT vitalalinefernandes deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis
AT lainettipatriciadefaria deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis
AT leisfilhoantoniofernando deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis
AT dalmolinfabiola deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis
AT eliasfabiana deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis
AT calazanssabrynagouveia deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis
AT fonsecaalvescarloseduardo deregulationofvegfr2andpdgfrexpressionandmicrovasculardensityinatriplenegativemodelofcaninemalignantmammarytumorswithlymphnodeorlungmetastasis