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Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms

Mammary gland tumors (MGTs) are common in dogs and show a variable clinical behavior that is difficult to predict. Currently, few immunohistochemical markers have been established to predict the prognosis of a canine MGT. However, p53 immunostaining has been variably reported to be prognostic for ca...

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Autores principales: Munday, John S, Ariyarathna, Harsha, Aberdein, Danielle, Thomson, Neroli A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466101/
https://www.ncbi.nlm.nih.gov/pubmed/30934592
http://dx.doi.org/10.3390/vetsci6010034
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author Munday, John S
Ariyarathna, Harsha
Aberdein, Danielle
Thomson, Neroli A
author_facet Munday, John S
Ariyarathna, Harsha
Aberdein, Danielle
Thomson, Neroli A
author_sort Munday, John S
collection PubMed
description Mammary gland tumors (MGTs) are common in dogs and show a variable clinical behavior that is difficult to predict. Currently, few immunohistochemical markers have been established to predict the prognosis of a canine MGT. However, p53 immunostaining has been variably reported to be prognostic for canine MGTs. Additionally, while p16(CDK2NA) protein (p16) immunostaining has been found to be prognostic for human breast cancers, this marker has never been evaluated as a prognostic marker for canine neoplasms. In the present study, the prognostic utility of p53 and p16 was evaluated in 35 canine malignant MGTs. It was observed that 19 (54%) dogs died due to their MGTs with an overall mean survival time (MST) of 882 days. Seven MGTs showed p53 immunostaining, but this was not significantly associated with death (4 of 7 vs. 15 of 28; p = 0.6) or MST (670 vs. 934 days; p = 0.57). Five dogs had MGTs with no p16 immunostaining, 28 MGTs had intermediate p16 immunostaining, and two MGTs had increased p16 immunostaining. Neither death due to MGT (4 of 5, 14 of 28, or 1 of 2; p = 0.28) nor MST (683, 927, and 307 days; p = 0.31) were significantly associated with p16 immunostaining. Interestingly, p53 immunostaining was significantly associated with an increase or loss of p16 immunostaining. This is the first time that p16 has been evaluated as a prognostic marker for canine neoplasms. While these results suggest that a proportion of canine MGTs develop by cellular mechanisms that alter both p53 and p16 expression, there was no evidence that defects in p53 or p16 alter the behavior of a MGT. Neither p53 nor p16 was found to significantly predict prognosis, although this could reflect the limited number of MGTs included in the study.
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spelling pubmed-64661012019-04-19 Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms Munday, John S Ariyarathna, Harsha Aberdein, Danielle Thomson, Neroli A Vet Sci Article Mammary gland tumors (MGTs) are common in dogs and show a variable clinical behavior that is difficult to predict. Currently, few immunohistochemical markers have been established to predict the prognosis of a canine MGT. However, p53 immunostaining has been variably reported to be prognostic for canine MGTs. Additionally, while p16(CDK2NA) protein (p16) immunostaining has been found to be prognostic for human breast cancers, this marker has never been evaluated as a prognostic marker for canine neoplasms. In the present study, the prognostic utility of p53 and p16 was evaluated in 35 canine malignant MGTs. It was observed that 19 (54%) dogs died due to their MGTs with an overall mean survival time (MST) of 882 days. Seven MGTs showed p53 immunostaining, but this was not significantly associated with death (4 of 7 vs. 15 of 28; p = 0.6) or MST (670 vs. 934 days; p = 0.57). Five dogs had MGTs with no p16 immunostaining, 28 MGTs had intermediate p16 immunostaining, and two MGTs had increased p16 immunostaining. Neither death due to MGT (4 of 5, 14 of 28, or 1 of 2; p = 0.28) nor MST (683, 927, and 307 days; p = 0.31) were significantly associated with p16 immunostaining. Interestingly, p53 immunostaining was significantly associated with an increase or loss of p16 immunostaining. This is the first time that p16 has been evaluated as a prognostic marker for canine neoplasms. While these results suggest that a proportion of canine MGTs develop by cellular mechanisms that alter both p53 and p16 expression, there was no evidence that defects in p53 or p16 alter the behavior of a MGT. Neither p53 nor p16 was found to significantly predict prognosis, although this could reflect the limited number of MGTs included in the study. MDPI 2019-03-25 /pmc/articles/PMC6466101/ /pubmed/30934592 http://dx.doi.org/10.3390/vetsci6010034 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Munday, John S
Ariyarathna, Harsha
Aberdein, Danielle
Thomson, Neroli A
Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms
title Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms
title_full Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms
title_fullStr Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms
title_full_unstemmed Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms
title_short Immunostaining for p53 and p16(CDKN2A) Protein Is Not Predictive of Prognosis for Dogs with Malignant Mammary Gland Neoplasms
title_sort immunostaining for p53 and p16(cdkn2a) protein is not predictive of prognosis for dogs with malignant mammary gland neoplasms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466101/
https://www.ncbi.nlm.nih.gov/pubmed/30934592
http://dx.doi.org/10.3390/vetsci6010034
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