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Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period

The main objective of this study was to assess PAG2 mRNA expression in maternal blood cells at the peri-implantation period in water buffalo; moreover, we wanted to evaluate the earliest time in which PAG-2 could be detected in maternal blood. Thirty-two lactating buffaloes artificially inseminated...

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Autores principales: Barbato, Olimpia, Guelfi, Gabriella, Menchetti, Laura, Brecchia, Gabriele, Melo de Sousa, Noelita, Canali, Claudio, Grandoni, Francesco, Scatà, Maria Carmela, De Matteis, Giovanna, Casano, Anna Beatrice, Beckers, Jean François, Barile, Vittoria Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466188/
https://www.ncbi.nlm.nih.gov/pubmed/30646632
http://dx.doi.org/10.3390/vetsci6010008
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author Barbato, Olimpia
Guelfi, Gabriella
Menchetti, Laura
Brecchia, Gabriele
Melo de Sousa, Noelita
Canali, Claudio
Grandoni, Francesco
Scatà, Maria Carmela
De Matteis, Giovanna
Casano, Anna Beatrice
Beckers, Jean François
Barile, Vittoria Lucia
author_facet Barbato, Olimpia
Guelfi, Gabriella
Menchetti, Laura
Brecchia, Gabriele
Melo de Sousa, Noelita
Canali, Claudio
Grandoni, Francesco
Scatà, Maria Carmela
De Matteis, Giovanna
Casano, Anna Beatrice
Beckers, Jean François
Barile, Vittoria Lucia
author_sort Barbato, Olimpia
collection PubMed
description The main objective of this study was to assess PAG2 mRNA expression in maternal blood cells at the peri-implantation period in water buffalo; moreover, we wanted to evaluate the earliest time in which PAG-2 could be detected in maternal blood. Thirty-two lactating buffaloes artificially inseminated (AI) were utilized. Blood was collected at Days 0, 14, 18, 28, 40 after AI (AI = day 0). Pregnancy was diagnosed by ultrasound at Days 28 and 40 post AI. Out of 32 buffaloes, 14 were pregnant (P group) and 18 were not pregnant (NP group). The plasma PAG-2 threshold of 1.0 ng/mL in the P group was reached at day 40 post AI. PAG2 mRNA expression differed between the P and NP groups, and was either evaluated in Peripheral Blood Mononuclear Cells (PBMC) or Polymorphonuclear Leukocytes (PMN), starting from day 14. However, both the estimated marginal means and multiple comparisons showed that PAG2 mRNA expression was higher in PMN than PBMC. In the present study, PAG-2 appeared in the blood (40 Days post AI), and an early expression of PAG2 mRNA at Day 14 post AI was also observed. Although further research is undoubtedly required, PAG2 mRNA in peripheral blood leukocytes could be using to better understand the role that PAGs play during pregnancy in buffalo.
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spelling pubmed-64661882019-04-19 Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period Barbato, Olimpia Guelfi, Gabriella Menchetti, Laura Brecchia, Gabriele Melo de Sousa, Noelita Canali, Claudio Grandoni, Francesco Scatà, Maria Carmela De Matteis, Giovanna Casano, Anna Beatrice Beckers, Jean François Barile, Vittoria Lucia Vet Sci Article The main objective of this study was to assess PAG2 mRNA expression in maternal blood cells at the peri-implantation period in water buffalo; moreover, we wanted to evaluate the earliest time in which PAG-2 could be detected in maternal blood. Thirty-two lactating buffaloes artificially inseminated (AI) were utilized. Blood was collected at Days 0, 14, 18, 28, 40 after AI (AI = day 0). Pregnancy was diagnosed by ultrasound at Days 28 and 40 post AI. Out of 32 buffaloes, 14 were pregnant (P group) and 18 were not pregnant (NP group). The plasma PAG-2 threshold of 1.0 ng/mL in the P group was reached at day 40 post AI. PAG2 mRNA expression differed between the P and NP groups, and was either evaluated in Peripheral Blood Mononuclear Cells (PBMC) or Polymorphonuclear Leukocytes (PMN), starting from day 14. However, both the estimated marginal means and multiple comparisons showed that PAG2 mRNA expression was higher in PMN than PBMC. In the present study, PAG-2 appeared in the blood (40 Days post AI), and an early expression of PAG2 mRNA at Day 14 post AI was also observed. Although further research is undoubtedly required, PAG2 mRNA in peripheral blood leukocytes could be using to better understand the role that PAGs play during pregnancy in buffalo. MDPI 2019-01-14 /pmc/articles/PMC6466188/ /pubmed/30646632 http://dx.doi.org/10.3390/vetsci6010008 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barbato, Olimpia
Guelfi, Gabriella
Menchetti, Laura
Brecchia, Gabriele
Melo de Sousa, Noelita
Canali, Claudio
Grandoni, Francesco
Scatà, Maria Carmela
De Matteis, Giovanna
Casano, Anna Beatrice
Beckers, Jean François
Barile, Vittoria Lucia
Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period
title Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period
title_full Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period
title_fullStr Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period
title_full_unstemmed Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period
title_short Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period
title_sort investigation of pag2 mrna expression in water buffalo peripheral blood mononuclear cells and polymorphonuclear leukocytes from maternal blood at the peri-implantation period
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466188/
https://www.ncbi.nlm.nih.gov/pubmed/30646632
http://dx.doi.org/10.3390/vetsci6010008
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