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Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product

Epstein–Barr virus (EBV) is a ubiquitous virus that causes infectious mononucleosis and several types of cancer, such as Burkitt lymphoma, T/NK-cell lymphoma, and nasopharyngeal carcinoma. As a herpesvirus, it encodes more than 80 genes, many of which have not been characterized. EBV BamHI S rightwa...

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Autores principales: Yanagi, Yusuke, Masud, H. M. Abdullah Al, Watanabe, Takahiro, Sato, Yoshitaka, Goshima, Fumi, Kimura, Hiroshi, Murata, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466199/
https://www.ncbi.nlm.nih.gov/pubmed/30901892
http://dx.doi.org/10.3390/v11030285
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author Yanagi, Yusuke
Masud, H. M. Abdullah Al
Watanabe, Takahiro
Sato, Yoshitaka
Goshima, Fumi
Kimura, Hiroshi
Murata, Takayuki
author_facet Yanagi, Yusuke
Masud, H. M. Abdullah Al
Watanabe, Takahiro
Sato, Yoshitaka
Goshima, Fumi
Kimura, Hiroshi
Murata, Takayuki
author_sort Yanagi, Yusuke
collection PubMed
description Epstein–Barr virus (EBV) is a ubiquitous virus that causes infectious mononucleosis and several types of cancer, such as Burkitt lymphoma, T/NK-cell lymphoma, and nasopharyngeal carcinoma. As a herpesvirus, it encodes more than 80 genes, many of which have not been characterized. EBV BamHI S rightward reading frame 1 (BSRF1) encodes a tegument protein that, unlike its homologs herpes simplex virus unique long 51 (UL51) and human cytomegalovirus UL71, has not been extensively investigated. To examine the role of BSRF1, we prepared knockout and revertant strains using the bacterial artificial chromosome system. Unexpectedly, the disruption of the gene had little or no effect on EBV lytic replication and the transformation of primary B cells. However, the knockdown of BSRF1 in B95-8 cells decreased progeny production. An immunofluorescence assay revealed that BSRF1 localized to the Golgi apparatus in the cytoplasm, as did its homologs. BSRF1 also associated with BamHI G leftward reading frame 3.5 (BGLF3.5), BamHI B rightward reading frame 2 (BBRF2), and BamHI A leftward reading frame 1 (BALF1), and BALF1 was incorporated into the tegument fraction with BSRF1. Taken together, our results indicate that BSRF1 plays a role in secondary envelopment or virion egress in the cytoplasm, as do its homolog genes.
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spelling pubmed-64661992019-04-18 Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product Yanagi, Yusuke Masud, H. M. Abdullah Al Watanabe, Takahiro Sato, Yoshitaka Goshima, Fumi Kimura, Hiroshi Murata, Takayuki Viruses Article Epstein–Barr virus (EBV) is a ubiquitous virus that causes infectious mononucleosis and several types of cancer, such as Burkitt lymphoma, T/NK-cell lymphoma, and nasopharyngeal carcinoma. As a herpesvirus, it encodes more than 80 genes, many of which have not been characterized. EBV BamHI S rightward reading frame 1 (BSRF1) encodes a tegument protein that, unlike its homologs herpes simplex virus unique long 51 (UL51) and human cytomegalovirus UL71, has not been extensively investigated. To examine the role of BSRF1, we prepared knockout and revertant strains using the bacterial artificial chromosome system. Unexpectedly, the disruption of the gene had little or no effect on EBV lytic replication and the transformation of primary B cells. However, the knockdown of BSRF1 in B95-8 cells decreased progeny production. An immunofluorescence assay revealed that BSRF1 localized to the Golgi apparatus in the cytoplasm, as did its homologs. BSRF1 also associated with BamHI G leftward reading frame 3.5 (BGLF3.5), BamHI B rightward reading frame 2 (BBRF2), and BamHI A leftward reading frame 1 (BALF1), and BALF1 was incorporated into the tegument fraction with BSRF1. Taken together, our results indicate that BSRF1 plays a role in secondary envelopment or virion egress in the cytoplasm, as do its homolog genes. MDPI 2019-03-21 /pmc/articles/PMC6466199/ /pubmed/30901892 http://dx.doi.org/10.3390/v11030285 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yanagi, Yusuke
Masud, H. M. Abdullah Al
Watanabe, Takahiro
Sato, Yoshitaka
Goshima, Fumi
Kimura, Hiroshi
Murata, Takayuki
Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product
title Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product
title_full Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product
title_fullStr Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product
title_full_unstemmed Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product
title_short Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product
title_sort initial characterization of the epstein–barr virus bsrf1 gene product
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466199/
https://www.ncbi.nlm.nih.gov/pubmed/30901892
http://dx.doi.org/10.3390/v11030285
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