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A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire

New technologies enable viral discovery in a diversity of hosts, providing insights into viral evolution. We used one such approach, the virome capture sequencing for vertebrate viruses (VirCapSeq-VERT) platform, on 21 samples originating from six dead Maxwell’s duikers (Philantomba maxwellii) from...

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Autores principales: Gogarten, Jan F., Ulrich, Markus, Bhuva, Nishit, Garcia, Joel, Jain, Komal, Lee, Bohyun, Löhrich, Therese, Oleynik, Alexandra, Couacy-Hymann, Emmanuel, Fuh Neba, Terence, Mishra, Nischay, Briese, Thomas, Calvignac-Spencer, Sébastien, Lipkin, W. Ian, Leendertz, Fabian H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466360/
https://www.ncbi.nlm.nih.gov/pubmed/30893858
http://dx.doi.org/10.3390/v11030279
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author Gogarten, Jan F.
Ulrich, Markus
Bhuva, Nishit
Garcia, Joel
Jain, Komal
Lee, Bohyun
Löhrich, Therese
Oleynik, Alexandra
Couacy-Hymann, Emmanuel
Fuh Neba, Terence
Mishra, Nischay
Briese, Thomas
Calvignac-Spencer, Sébastien
Lipkin, W. Ian
Leendertz, Fabian H.
author_facet Gogarten, Jan F.
Ulrich, Markus
Bhuva, Nishit
Garcia, Joel
Jain, Komal
Lee, Bohyun
Löhrich, Therese
Oleynik, Alexandra
Couacy-Hymann, Emmanuel
Fuh Neba, Terence
Mishra, Nischay
Briese, Thomas
Calvignac-Spencer, Sébastien
Lipkin, W. Ian
Leendertz, Fabian H.
author_sort Gogarten, Jan F.
collection PubMed
description New technologies enable viral discovery in a diversity of hosts, providing insights into viral evolution. We used one such approach, the virome capture sequencing for vertebrate viruses (VirCapSeq-VERT) platform, on 21 samples originating from six dead Maxwell’s duikers (Philantomba maxwellii) from Taï National Park, Côte d’Ivoire. We detected the presence of an orthohepadnavirus in one animal and characterized its 3128 bp genome. The highest viral copy numbers were detected in the spleen, followed by the lung, blood, and liver, with the lowest copy numbers in the kidney and heart; the virus was not detected in the jejunum. Viral copy numbers in the blood were in the range known from humans with active chronic infections leading to liver histolytic damage, suggesting this virus could be pathogenic in duikers, though many orthohepadnaviruses appear to be apathogenic in other hosts, precluding a formal test of this hypothesis. The virus was not detected in 29 other dead duiker samples from the Côte d’Ivoire and Central African Republic, suggesting either a spillover event or a low prevalence in these populations. Phylogenetic analysis placed the virus as a divergent member of the mammalian clade of orthohepadnaviruses, though its relationship to other orthohepadnaviruses remains uncertain. This represents the first orthohepadnavirus described in an artiodactyl. We have tentatively named this new member of the genus Orthohepadnavirus (family Hepadnaviridae), Taï Forest hepadnavirus. Further studies are needed to determine whether it, or some close relatives, are present in a broader range of artiodactyls, including livestock.
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spelling pubmed-64663602019-04-18 A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire Gogarten, Jan F. Ulrich, Markus Bhuva, Nishit Garcia, Joel Jain, Komal Lee, Bohyun Löhrich, Therese Oleynik, Alexandra Couacy-Hymann, Emmanuel Fuh Neba, Terence Mishra, Nischay Briese, Thomas Calvignac-Spencer, Sébastien Lipkin, W. Ian Leendertz, Fabian H. Viruses Communication New technologies enable viral discovery in a diversity of hosts, providing insights into viral evolution. We used one such approach, the virome capture sequencing for vertebrate viruses (VirCapSeq-VERT) platform, on 21 samples originating from six dead Maxwell’s duikers (Philantomba maxwellii) from Taï National Park, Côte d’Ivoire. We detected the presence of an orthohepadnavirus in one animal and characterized its 3128 bp genome. The highest viral copy numbers were detected in the spleen, followed by the lung, blood, and liver, with the lowest copy numbers in the kidney and heart; the virus was not detected in the jejunum. Viral copy numbers in the blood were in the range known from humans with active chronic infections leading to liver histolytic damage, suggesting this virus could be pathogenic in duikers, though many orthohepadnaviruses appear to be apathogenic in other hosts, precluding a formal test of this hypothesis. The virus was not detected in 29 other dead duiker samples from the Côte d’Ivoire and Central African Republic, suggesting either a spillover event or a low prevalence in these populations. Phylogenetic analysis placed the virus as a divergent member of the mammalian clade of orthohepadnaviruses, though its relationship to other orthohepadnaviruses remains uncertain. This represents the first orthohepadnavirus described in an artiodactyl. We have tentatively named this new member of the genus Orthohepadnavirus (family Hepadnaviridae), Taï Forest hepadnavirus. Further studies are needed to determine whether it, or some close relatives, are present in a broader range of artiodactyls, including livestock. MDPI 2019-03-19 /pmc/articles/PMC6466360/ /pubmed/30893858 http://dx.doi.org/10.3390/v11030279 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gogarten, Jan F.
Ulrich, Markus
Bhuva, Nishit
Garcia, Joel
Jain, Komal
Lee, Bohyun
Löhrich, Therese
Oleynik, Alexandra
Couacy-Hymann, Emmanuel
Fuh Neba, Terence
Mishra, Nischay
Briese, Thomas
Calvignac-Spencer, Sébastien
Lipkin, W. Ian
Leendertz, Fabian H.
A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire
title A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire
title_full A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire
title_fullStr A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire
title_full_unstemmed A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire
title_short A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire
title_sort novel orthohepadnavirus identified in a dead maxwell’s duiker (philantomba maxwellii) in taï national park, côte d’ivoire
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466360/
https://www.ncbi.nlm.nih.gov/pubmed/30893858
http://dx.doi.org/10.3390/v11030279
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