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3D Printing for Bio-Synthetic Biliary Stents
Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466390/ https://www.ncbi.nlm.nih.gov/pubmed/30744131 http://dx.doi.org/10.3390/bioengineering6010016 |
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author | Boyer, Christen J. Boktor, Moheb Samant, Hrishikesh White, Luke A. Wang, Yuping Ballard, David H. Huebert, Robert C. Woerner, Jennifer E. Ghali, Ghali E. Alexander, Jonathan S. |
author_facet | Boyer, Christen J. Boktor, Moheb Samant, Hrishikesh White, Luke A. Wang, Yuping Ballard, David H. Huebert, Robert C. Woerner, Jennifer E. Ghali, Ghali E. Alexander, Jonathan S. |
author_sort | Boyer, Christen J. |
collection | PubMed |
description | Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques. |
format | Online Article Text |
id | pubmed-6466390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64663902019-04-19 3D Printing for Bio-Synthetic Biliary Stents Boyer, Christen J. Boktor, Moheb Samant, Hrishikesh White, Luke A. Wang, Yuping Ballard, David H. Huebert, Robert C. Woerner, Jennifer E. Ghali, Ghali E. Alexander, Jonathan S. Bioengineering (Basel) Article Three-dimensional (3D) printing is an additive manufacturing method that holds great potential in a variety of future patient-specific medical technologies. This project validated a novel crosslinked polyvinyl alcohol (XL-PVA) 3D printed stent infused with collagen, human placental mesenchymal stem cells (PMSCs), and cholangiocytes. The biofabrication method in the present study examined 3D printing and collagen injection molding for rapid prototyping of customized living biliary stents with clinical applications in the setting of malignant and benign bile duct obstructions. XL-PVA stents showed hydrophilic swelling and addition of radiocontrast to the stent matrix improved radiographic opacity. Collagen loaded with PMSCs contracted tightly around hydrophilic stents and dense choloangiocyte coatings were verified through histology and fluorescence microscopy. It is anticipated that design elements used in these stents may enable appropriate stent placement, provide protection of the stent-stem cell matrix against bile constituents, and potentially limit biofilm development. Overall, this approach may allow physicians to create personalized bio-integrating stents for use in biliary procedures and lays a foundation for new patient-specific stent fabrication techniques. MDPI 2019-02-09 /pmc/articles/PMC6466390/ /pubmed/30744131 http://dx.doi.org/10.3390/bioengineering6010016 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Boyer, Christen J. Boktor, Moheb Samant, Hrishikesh White, Luke A. Wang, Yuping Ballard, David H. Huebert, Robert C. Woerner, Jennifer E. Ghali, Ghali E. Alexander, Jonathan S. 3D Printing for Bio-Synthetic Biliary Stents |
title | 3D Printing for Bio-Synthetic Biliary Stents |
title_full | 3D Printing for Bio-Synthetic Biliary Stents |
title_fullStr | 3D Printing for Bio-Synthetic Biliary Stents |
title_full_unstemmed | 3D Printing for Bio-Synthetic Biliary Stents |
title_short | 3D Printing for Bio-Synthetic Biliary Stents |
title_sort | 3d printing for bio-synthetic biliary stents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466390/ https://www.ncbi.nlm.nih.gov/pubmed/30744131 http://dx.doi.org/10.3390/bioengineering6010016 |
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