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Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis

Hazardous organisms may thrive on surfaces that are often exposed to human contact, including children’s library books. In this study, swab samples were taken from 42 children’s books collected from four public libraries in Texas and California. Samples were then cultivated in brain–heart infusion (...

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Autores principales: Jung, Ryan Hyunjae, Kim, Minzae, Bhatt, Bhoomi, Choi, Jong Min, Roh, Jung H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466425/
https://www.ncbi.nlm.nih.gov/pubmed/30875719
http://dx.doi.org/10.3390/ijerph16060912
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author Jung, Ryan Hyunjae
Kim, Minzae
Bhatt, Bhoomi
Choi, Jong Min
Roh, Jung H.
author_facet Jung, Ryan Hyunjae
Kim, Minzae
Bhatt, Bhoomi
Choi, Jong Min
Roh, Jung H.
author_sort Jung, Ryan Hyunjae
collection PubMed
description Hazardous organisms may thrive on surfaces that are often exposed to human contact, including children’s library books. In this study, swab samples were taken from 42 children’s books collected from four public libraries in Texas and California. Samples were then cultivated in brain–heart infusion (BHI) medium and then in Luria broth (LB) medium containing either ampicillin or kanamycin. All 42 samples (100%) were positive for bacterial growth in normal BHI medium. Furthermore, 35 samples (83.3%) and 20 samples (47.6%) in total were positive in LB medium containing ampicillin or kanamycin, respectively. Bacterial populations were then identified in samples using an Orbitrap Fusion™ Tribrid ™ mass spectrometer, a state-of-the-art proteomic analysis tool. Identified bacterial species grown in ampicillin included Bacillus, Acinetobacter, Pseudomonas, Staphylococcus, Enterobacter, Klebsiella, Serratia, Streptococcus, Escherichia, Salmonella, and Enterococcus. In contrast, identified bacteria grown in kanamycin included Staphylococcus, Streptococcus, Enterococcus, and Bacillus. The presences of pathogenic bacteria species were also confirmed. The results of this study warrant follow up studies to assess the potential health risks of identified pathogens. This study demonstrates the utility of proteomics in identifying environmental pathogenic bacteria for specific public health risk evaluations.
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spelling pubmed-64664252019-04-22 Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis Jung, Ryan Hyunjae Kim, Minzae Bhatt, Bhoomi Choi, Jong Min Roh, Jung H. Int J Environ Res Public Health Article Hazardous organisms may thrive on surfaces that are often exposed to human contact, including children’s library books. In this study, swab samples were taken from 42 children’s books collected from four public libraries in Texas and California. Samples were then cultivated in brain–heart infusion (BHI) medium and then in Luria broth (LB) medium containing either ampicillin or kanamycin. All 42 samples (100%) were positive for bacterial growth in normal BHI medium. Furthermore, 35 samples (83.3%) and 20 samples (47.6%) in total were positive in LB medium containing ampicillin or kanamycin, respectively. Bacterial populations were then identified in samples using an Orbitrap Fusion™ Tribrid ™ mass spectrometer, a state-of-the-art proteomic analysis tool. Identified bacterial species grown in ampicillin included Bacillus, Acinetobacter, Pseudomonas, Staphylococcus, Enterobacter, Klebsiella, Serratia, Streptococcus, Escherichia, Salmonella, and Enterococcus. In contrast, identified bacteria grown in kanamycin included Staphylococcus, Streptococcus, Enterococcus, and Bacillus. The presences of pathogenic bacteria species were also confirmed. The results of this study warrant follow up studies to assess the potential health risks of identified pathogens. This study demonstrates the utility of proteomics in identifying environmental pathogenic bacteria for specific public health risk evaluations. MDPI 2019-03-14 2019-03 /pmc/articles/PMC6466425/ /pubmed/30875719 http://dx.doi.org/10.3390/ijerph16060912 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jung, Ryan Hyunjae
Kim, Minzae
Bhatt, Bhoomi
Choi, Jong Min
Roh, Jung H.
Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis
title Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis
title_full Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis
title_fullStr Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis
title_full_unstemmed Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis
title_short Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis
title_sort identification of pathogenic bacteria from public libraries via proteomics analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466425/
https://www.ncbi.nlm.nih.gov/pubmed/30875719
http://dx.doi.org/10.3390/ijerph16060912
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