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Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum

We previously determined that virions of Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 (SsHADV-1) could directly infect hyphae of Sclerotinia sclerotiorum, resulting in hypovirulence of the fungal host. However, the molecular mechanisms of SsHADV-1 virions disruption of the fungal ce...

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Autores principales: Ding, Feng, Cheng, Jiasen, Fu, Yanping, Chen, Tao, Li, Bo, Jiang, Daohong, Xie, Jiatao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466436/
https://www.ncbi.nlm.nih.gov/pubmed/30893849
http://dx.doi.org/10.3390/v11030278
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author Ding, Feng
Cheng, Jiasen
Fu, Yanping
Chen, Tao
Li, Bo
Jiang, Daohong
Xie, Jiatao
author_facet Ding, Feng
Cheng, Jiasen
Fu, Yanping
Chen, Tao
Li, Bo
Jiang, Daohong
Xie, Jiatao
author_sort Ding, Feng
collection PubMed
description We previously determined that virions of Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 (SsHADV-1) could directly infect hyphae of Sclerotinia sclerotiorum, resulting in hypovirulence of the fungal host. However, the molecular mechanisms of SsHADV-1 virions disruption of the fungal cell wall barrier and entrance into the host cell are still unclear. To investigate the early response of S. sclerotiorum to SsHADV-1 infection, S. sclerotiorum hyphae were inoculated with purified SsHADV-1 virions. The pre- and post-infection hyphae were collected at one–three hours post-inoculation for transcriptome analysis. Further, bioinformatic analysis showed that differentially expressed genes (DEGs) regulated by SsHADV-1 infection were identified in S. sclerotiorum. In total, 187 genes were differentially expressed, consisting of more up-regulated (114) than down-regulated (73) genes. The identified DEGs were involved in several important pathways. Metabolic processes, biosynthesis of antibiotics, and secondary metabolites were the most affected categories in S. sclerotiorum upon SsHADV-1 infection. Cell structure analysis suggested that 26% of the total DEGs were related to membrane tissues. Furthermore, 10 and 27 DEGs were predicted to be located in the cell membrane and mitochondria, respectively. Gene ontology enrichment analyses of the DEGs were performed, followed by functional annotation of the genes. Interestingly, one third of the annotated functional DEGs could be involved in the Ras-small G protein signal transduction pathway. These results revealed that SsHADV-1 virions may be able to bind host membrane proteins and influence signal transduction through Ras-small G protein-coupled receptors during early infection, providing new insight towards the molecular mechanisms of virions infection in S. sclerotiorum.
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spelling pubmed-64664362019-04-18 Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum Ding, Feng Cheng, Jiasen Fu, Yanping Chen, Tao Li, Bo Jiang, Daohong Xie, Jiatao Viruses Article We previously determined that virions of Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 (SsHADV-1) could directly infect hyphae of Sclerotinia sclerotiorum, resulting in hypovirulence of the fungal host. However, the molecular mechanisms of SsHADV-1 virions disruption of the fungal cell wall barrier and entrance into the host cell are still unclear. To investigate the early response of S. sclerotiorum to SsHADV-1 infection, S. sclerotiorum hyphae were inoculated with purified SsHADV-1 virions. The pre- and post-infection hyphae were collected at one–three hours post-inoculation for transcriptome analysis. Further, bioinformatic analysis showed that differentially expressed genes (DEGs) regulated by SsHADV-1 infection were identified in S. sclerotiorum. In total, 187 genes were differentially expressed, consisting of more up-regulated (114) than down-regulated (73) genes. The identified DEGs were involved in several important pathways. Metabolic processes, biosynthesis of antibiotics, and secondary metabolites were the most affected categories in S. sclerotiorum upon SsHADV-1 infection. Cell structure analysis suggested that 26% of the total DEGs were related to membrane tissues. Furthermore, 10 and 27 DEGs were predicted to be located in the cell membrane and mitochondria, respectively. Gene ontology enrichment analyses of the DEGs were performed, followed by functional annotation of the genes. Interestingly, one third of the annotated functional DEGs could be involved in the Ras-small G protein signal transduction pathway. These results revealed that SsHADV-1 virions may be able to bind host membrane proteins and influence signal transduction through Ras-small G protein-coupled receptors during early infection, providing new insight towards the molecular mechanisms of virions infection in S. sclerotiorum. MDPI 2019-03-19 /pmc/articles/PMC6466436/ /pubmed/30893849 http://dx.doi.org/10.3390/v11030278 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Feng
Cheng, Jiasen
Fu, Yanping
Chen, Tao
Li, Bo
Jiang, Daohong
Xie, Jiatao
Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum
title Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum
title_full Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum
title_fullStr Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum
title_full_unstemmed Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum
title_short Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum
title_sort early transcriptional response to dna virus infection in sclerotinia sclerotiorum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466436/
https://www.ncbi.nlm.nih.gov/pubmed/30893849
http://dx.doi.org/10.3390/v11030278
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