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Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease

BACKGROUND: To investigate the diagnostic value of the interferon-γ release assay (IGRA) for detecting tuberculosis (TB) infection in patients with Behçet’s disease (BD). METHODS: We retrospective analyzed the data collected from 173 BD patients hospitalized between 2010 and 2015. Ninety-nine health...

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Autores principales: Wu, Xiuhua, Chen, Pang, Wei, Wei, Zhou, Mengyu, Li, Chaoran, Liu, Jinjing, Zhao, Lidan, Zhang, Lifan, Zhao, Yan, Zeng, Xiaofeng, Liu, Xiaoqing, Zheng, Wenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466763/
https://www.ncbi.nlm.nih.gov/pubmed/30987605
http://dx.doi.org/10.1186/s12879-019-3954-y
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author Wu, Xiuhua
Chen, Pang
Wei, Wei
Zhou, Mengyu
Li, Chaoran
Liu, Jinjing
Zhao, Lidan
Zhang, Lifan
Zhao, Yan
Zeng, Xiaofeng
Liu, Xiaoqing
Zheng, Wenjie
author_facet Wu, Xiuhua
Chen, Pang
Wei, Wei
Zhou, Mengyu
Li, Chaoran
Liu, Jinjing
Zhao, Lidan
Zhang, Lifan
Zhao, Yan
Zeng, Xiaofeng
Liu, Xiaoqing
Zheng, Wenjie
author_sort Wu, Xiuhua
collection PubMed
description BACKGROUND: To investigate the diagnostic value of the interferon-γ release assay (IGRA) for detecting tuberculosis (TB) infection in patients with Behçet’s disease (BD). METHODS: We retrospective analyzed the data collected from 173 BD patients hospitalized between 2010 and 2015. Ninety-nine healthy volunteers were enrolled as a control group. IGRA was performed using T-SPOT.TB. The diagnosis of active TB (ATB) was based on clinical, radiological, microbiological, histopathological information and the response to anti-TB therapy. Latent TB (LTB) infection was defined as asymptomatic patients with positive T-SPOT.TB. RESULTS: TB infection was documented in 59 BD patients (34.1%). The sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio and negative likelihood ratio of T-SPOT.TB for the diagnosis of ATB were 88.9%, 74.8%, 29.1%, 98.3%, 3.53 and 0.15, respectively. The receiver-operating-characteristic curve demonstrated that spot-forming cells (SFCs) of 70/10(6) PBMC was the optimal cutoff for diagnosing ATB, with an area under the curve of 0.891. Furthermore, the median SFCs in ATB group was significantly higher than those in LTB infection (466/10(6) PBMC vs. 68/10(6) PBMC, p = 0.007) or previous TB infection (466/10(6) PBMC vs. 96/10(6) PBMC, p = 0.018). A significant discrepancy between T-SPOT.TB and tuberculin skin test was noted (kappa coefficient = 0.391, p = 0.002). CONCLUSIONS: T-SPOT.TB, an IGRA, may assist in the diagnosis of ATB in BD patients, and the higher SFCs suggest ATB in BD patients.
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spelling pubmed-64667632019-04-22 Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease Wu, Xiuhua Chen, Pang Wei, Wei Zhou, Mengyu Li, Chaoran Liu, Jinjing Zhao, Lidan Zhang, Lifan Zhao, Yan Zeng, Xiaofeng Liu, Xiaoqing Zheng, Wenjie BMC Infect Dis Research Article BACKGROUND: To investigate the diagnostic value of the interferon-γ release assay (IGRA) for detecting tuberculosis (TB) infection in patients with Behçet’s disease (BD). METHODS: We retrospective analyzed the data collected from 173 BD patients hospitalized between 2010 and 2015. Ninety-nine healthy volunteers were enrolled as a control group. IGRA was performed using T-SPOT.TB. The diagnosis of active TB (ATB) was based on clinical, radiological, microbiological, histopathological information and the response to anti-TB therapy. Latent TB (LTB) infection was defined as asymptomatic patients with positive T-SPOT.TB. RESULTS: TB infection was documented in 59 BD patients (34.1%). The sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio and negative likelihood ratio of T-SPOT.TB for the diagnosis of ATB were 88.9%, 74.8%, 29.1%, 98.3%, 3.53 and 0.15, respectively. The receiver-operating-characteristic curve demonstrated that spot-forming cells (SFCs) of 70/10(6) PBMC was the optimal cutoff for diagnosing ATB, with an area under the curve of 0.891. Furthermore, the median SFCs in ATB group was significantly higher than those in LTB infection (466/10(6) PBMC vs. 68/10(6) PBMC, p = 0.007) or previous TB infection (466/10(6) PBMC vs. 96/10(6) PBMC, p = 0.018). A significant discrepancy between T-SPOT.TB and tuberculin skin test was noted (kappa coefficient = 0.391, p = 0.002). CONCLUSIONS: T-SPOT.TB, an IGRA, may assist in the diagnosis of ATB in BD patients, and the higher SFCs suggest ATB in BD patients. BioMed Central 2019-04-15 /pmc/articles/PMC6466763/ /pubmed/30987605 http://dx.doi.org/10.1186/s12879-019-3954-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wu, Xiuhua
Chen, Pang
Wei, Wei
Zhou, Mengyu
Li, Chaoran
Liu, Jinjing
Zhao, Lidan
Zhang, Lifan
Zhao, Yan
Zeng, Xiaofeng
Liu, Xiaoqing
Zheng, Wenjie
Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease
title Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease
title_full Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease
title_fullStr Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease
title_full_unstemmed Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease
title_short Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease
title_sort diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with behçet’s disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466763/
https://www.ncbi.nlm.nih.gov/pubmed/30987605
http://dx.doi.org/10.1186/s12879-019-3954-y
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