Cargando…
Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications
Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until pl...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467423/ https://www.ncbi.nlm.nih.gov/pubmed/30946745 http://dx.doi.org/10.1371/journal.pgen.1008065 |
_version_ | 1783411271515766784 |
---|---|
author | Richter, René Kinoshita, Atsuko Vincent, Coral Martinez-Gallegos, Rafael Gao, He van Driel, Annabel D. Hyun, Youbong Mateos, Julieta L. Coupland, George |
author_facet | Richter, René Kinoshita, Atsuko Vincent, Coral Martinez-Gallegos, Rafael Gao, He van Driel, Annabel D. Hyun, Youbong Mateos, Julieta L. Coupland, George |
author_sort | Richter, René |
collection | PubMed |
description | Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until plants are exposed to winter cold. However, the target genes of FLC have not been thoroughly described, and our understanding of the mechanisms by which FLC represses transcription of these targets and how this repression is overcome during floral transition is still fragmentary. Here, we identify and characterize TARGET OF FLC AND SVP1 (TFS1), a novel target gene of FLC and its interacting protein SHORT VEGETATIVE PHASE (SVP). TFS1 encodes a B3-type transcription factor, and we show that tfs1 mutants are later flowering than wild-type, particularly under short days. FLC and SVP repress TFS1 transcription leading to deposition of trimethylation of Iysine 27 of histone 3 (H3K27me3) by the Polycomb Repressive Complex 2 at the TFS1 locus. During floral transition, after downregulation of FLC by cold, TFS1 transcription is promoted by SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), a MADS box protein encoded by another target of FLC/SVP. SOC1 opposes PRC function at TFS1 through recruitment of the histone demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) and the SWI/SNF chromatin remodeler ATPase BRAHMA (BRM). This recruitment of BRM is also strictly required for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) binding at TFS1 to coordinate RNAPII recruitment through the Mediator complex. Thus, we show that antagonistic chromatin modifications mediated by different MADS box transcription factor complexes play a crucial role in defining the temporal and spatial patterns of transcription of genes within a network of interactions downstream of FLC/SVP during floral transition. |
format | Online Article Text |
id | pubmed-6467423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64674232019-05-03 Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications Richter, René Kinoshita, Atsuko Vincent, Coral Martinez-Gallegos, Rafael Gao, He van Driel, Annabel D. Hyun, Youbong Mateos, Julieta L. Coupland, George PLoS Genet Research Article Integration of environmental and endogenous cues at plant shoot meristems determines the timing of flowering and reproductive development. The MADS box transcription factor FLOWERING LOCUS C (FLC) of Arabidopsis thaliana is an important repressor of floral transition, which blocks flowering until plants are exposed to winter cold. However, the target genes of FLC have not been thoroughly described, and our understanding of the mechanisms by which FLC represses transcription of these targets and how this repression is overcome during floral transition is still fragmentary. Here, we identify and characterize TARGET OF FLC AND SVP1 (TFS1), a novel target gene of FLC and its interacting protein SHORT VEGETATIVE PHASE (SVP). TFS1 encodes a B3-type transcription factor, and we show that tfs1 mutants are later flowering than wild-type, particularly under short days. FLC and SVP repress TFS1 transcription leading to deposition of trimethylation of Iysine 27 of histone 3 (H3K27me3) by the Polycomb Repressive Complex 2 at the TFS1 locus. During floral transition, after downregulation of FLC by cold, TFS1 transcription is promoted by SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), a MADS box protein encoded by another target of FLC/SVP. SOC1 opposes PRC function at TFS1 through recruitment of the histone demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) and the SWI/SNF chromatin remodeler ATPase BRAHMA (BRM). This recruitment of BRM is also strictly required for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) binding at TFS1 to coordinate RNAPII recruitment through the Mediator complex. Thus, we show that antagonistic chromatin modifications mediated by different MADS box transcription factor complexes play a crucial role in defining the temporal and spatial patterns of transcription of genes within a network of interactions downstream of FLC/SVP during floral transition. Public Library of Science 2019-04-04 /pmc/articles/PMC6467423/ /pubmed/30946745 http://dx.doi.org/10.1371/journal.pgen.1008065 Text en © 2019 Richter et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Richter, René Kinoshita, Atsuko Vincent, Coral Martinez-Gallegos, Rafael Gao, He van Driel, Annabel D. Hyun, Youbong Mateos, Julieta L. Coupland, George Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications |
title | Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications |
title_full | Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications |
title_fullStr | Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications |
title_full_unstemmed | Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications |
title_short | Floral regulators FLC and SOC1 directly regulate expression of the B3-type transcription factor TARGET OF FLC AND SVP 1 at the Arabidopsis shoot apex via antagonistic chromatin modifications |
title_sort | floral regulators flc and soc1 directly regulate expression of the b3-type transcription factor target of flc and svp 1 at the arabidopsis shoot apex via antagonistic chromatin modifications |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467423/ https://www.ncbi.nlm.nih.gov/pubmed/30946745 http://dx.doi.org/10.1371/journal.pgen.1008065 |
work_keys_str_mv | AT richterrene floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT kinoshitaatsuko floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT vincentcoral floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT martinezgallegosrafael floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT gaohe floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT vandrielannabeld floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT hyunyoubong floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT mateosjulietal floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications AT couplandgeorge floralregulatorsflcandsoc1directlyregulateexpressionoftheb3typetranscriptionfactortargetofflcandsvp1atthearabidopsisshootapexviaantagonisticchromatinmodifications |