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First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae)

The taxonomic identification of Lonchothrix emiliae (Rodentia, Echimyidae, Eumysopinae) is problematic because of the overlap of morphological characters with its sister clade represented by species in the genus Mesomys which, like L. emiliae, is distributed throughout the Amazonian biome. Cytogenet...

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Autores principales: Dias de Oliveira, Leony, Oliveira da Silva, Willam, Rodrigues da Costa, Marlyson Jeremias, Sampaio, Iracilda, Pieczarka, Julio Cesar, Nagamachi, Cleusa Yoshiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467446/
https://www.ncbi.nlm.nih.gov/pubmed/30990834
http://dx.doi.org/10.1371/journal.pone.0215239
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author Dias de Oliveira, Leony
Oliveira da Silva, Willam
Rodrigues da Costa, Marlyson Jeremias
Sampaio, Iracilda
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author_facet Dias de Oliveira, Leony
Oliveira da Silva, Willam
Rodrigues da Costa, Marlyson Jeremias
Sampaio, Iracilda
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
author_sort Dias de Oliveira, Leony
collection PubMed
description The taxonomic identification of Lonchothrix emiliae (Rodentia, Echimyidae, Eumysopinae) is problematic because of the overlap of morphological characters with its sister clade represented by species in the genus Mesomys which, like L. emiliae, is distributed throughout the Amazonian biome. Cytogenetic studies reported the karyotype of L. emiliae as 2n = 60/FN = 116, but this karyotype and samples were later designated as M. hispidus. To evaluate the karyotype diversity of Lonchothrix and Mesomys, and to provide data useful as karyological diagnostic characters, in the present study we made a comparative analysis of specimens of L. emiliae and M. stimulax collected from two Brazilian Amazonian localities, using C-banding, G-banding, FISH using rDNA 45S and telomeric probes, and Cytochrome-b (Cytb) sequences. The results indicate that L. emiliae has 2n = 64♀, 65♂/FN = 124 and a multiple sexual system (XX/XY(1)Y(2)), while M. stimulax has 2n = 60/FN = 116. The Neo-X system found in L. emiliae also occurs in two Proechimys species, but cytogenetic analysis indicated an independent origin for these systems. The rDNA 45S analysis showed interstitial signals at one autosomal pair for each species, while an ITS found in L. emiliae was not coincident with the NOR. The molecular analysis confirmed Lonchothrix and Mesomys are sister genera, and the high level of intraspecific genetic divergence (7.1%) in M. stimulax suggests that it may be a species complex.
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spelling pubmed-64674462019-05-03 First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae) Dias de Oliveira, Leony Oliveira da Silva, Willam Rodrigues da Costa, Marlyson Jeremias Sampaio, Iracilda Pieczarka, Julio Cesar Nagamachi, Cleusa Yoshiko PLoS One Research Article The taxonomic identification of Lonchothrix emiliae (Rodentia, Echimyidae, Eumysopinae) is problematic because of the overlap of morphological characters with its sister clade represented by species in the genus Mesomys which, like L. emiliae, is distributed throughout the Amazonian biome. Cytogenetic studies reported the karyotype of L. emiliae as 2n = 60/FN = 116, but this karyotype and samples were later designated as M. hispidus. To evaluate the karyotype diversity of Lonchothrix and Mesomys, and to provide data useful as karyological diagnostic characters, in the present study we made a comparative analysis of specimens of L. emiliae and M. stimulax collected from two Brazilian Amazonian localities, using C-banding, G-banding, FISH using rDNA 45S and telomeric probes, and Cytochrome-b (Cytb) sequences. The results indicate that L. emiliae has 2n = 64♀, 65♂/FN = 124 and a multiple sexual system (XX/XY(1)Y(2)), while M. stimulax has 2n = 60/FN = 116. The Neo-X system found in L. emiliae also occurs in two Proechimys species, but cytogenetic analysis indicated an independent origin for these systems. The rDNA 45S analysis showed interstitial signals at one autosomal pair for each species, while an ITS found in L. emiliae was not coincident with the NOR. The molecular analysis confirmed Lonchothrix and Mesomys are sister genera, and the high level of intraspecific genetic divergence (7.1%) in M. stimulax suggests that it may be a species complex. Public Library of Science 2019-04-16 /pmc/articles/PMC6467446/ /pubmed/30990834 http://dx.doi.org/10.1371/journal.pone.0215239 Text en © 2019 Dias de Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dias de Oliveira, Leony
Oliveira da Silva, Willam
Rodrigues da Costa, Marlyson Jeremias
Sampaio, Iracilda
Pieczarka, Julio Cesar
Nagamachi, Cleusa Yoshiko
First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae)
title First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae)
title_full First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae)
title_fullStr First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae)
title_full_unstemmed First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae)
title_short First cytogenetic information for Lonchothrix emiliae and taxonomic implications for the genus taxa Lonchothrix + Mesomys (Rodentia, Echimyidae, Eumysopinae)
title_sort first cytogenetic information for lonchothrix emiliae and taxonomic implications for the genus taxa lonchothrix + mesomys (rodentia, echimyidae, eumysopinae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467446/
https://www.ncbi.nlm.nih.gov/pubmed/30990834
http://dx.doi.org/10.1371/journal.pone.0215239
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