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Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan

A comprehensive description of the phenotypic changes during cellular aging is key towards unraveling its causal forces. Previously, we mapped age-related changes in the proteome and transcriptome (Janssens et al., 2015). Here, employing the same experimental procedure and model-based inference, we...

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Autores principales: Leupold, Simeon, Hubmann, Georg, Litsios, Athanasios, Meinema, Anne C, Takhaveev, Vakil, Papagiannakis, Alexandros, Niebel, Bastian, Janssens, Georges, Siegel, David, Heinemann, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467564/
https://www.ncbi.nlm.nih.gov/pubmed/30963997
http://dx.doi.org/10.7554/eLife.41046
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author Leupold, Simeon
Hubmann, Georg
Litsios, Athanasios
Meinema, Anne C
Takhaveev, Vakil
Papagiannakis, Alexandros
Niebel, Bastian
Janssens, Georges
Siegel, David
Heinemann, Matthias
author_facet Leupold, Simeon
Hubmann, Georg
Litsios, Athanasios
Meinema, Anne C
Takhaveev, Vakil
Papagiannakis, Alexandros
Niebel, Bastian
Janssens, Georges
Siegel, David
Heinemann, Matthias
author_sort Leupold, Simeon
collection PubMed
description A comprehensive description of the phenotypic changes during cellular aging is key towards unraveling its causal forces. Previously, we mapped age-related changes in the proteome and transcriptome (Janssens et al., 2015). Here, employing the same experimental procedure and model-based inference, we generate a comprehensive account of metabolic changes during the replicative life of Saccharomyces cerevisiae. With age, we found decreasing metabolite levels, decreasing growth and substrate uptake rates accompanied by a switch from aerobic fermentation to respiration, with glycerol and acetate production. The identified metabolic fluxes revealed an increase in redox cofactor turnover, likely to combat increased production of reactive oxygen species. The metabolic changes are possibly a result of the age-associated decrease in surface area per cell volume. With metabolism being an important factor of the cellular phenotype, this work complements our recent mapping of the transcriptomic and proteomic changes towards a holistic description of the cellular phenotype during aging.
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spelling pubmed-64675642019-04-17 Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan Leupold, Simeon Hubmann, Georg Litsios, Athanasios Meinema, Anne C Takhaveev, Vakil Papagiannakis, Alexandros Niebel, Bastian Janssens, Georges Siegel, David Heinemann, Matthias eLife Cell Biology A comprehensive description of the phenotypic changes during cellular aging is key towards unraveling its causal forces. Previously, we mapped age-related changes in the proteome and transcriptome (Janssens et al., 2015). Here, employing the same experimental procedure and model-based inference, we generate a comprehensive account of metabolic changes during the replicative life of Saccharomyces cerevisiae. With age, we found decreasing metabolite levels, decreasing growth and substrate uptake rates accompanied by a switch from aerobic fermentation to respiration, with glycerol and acetate production. The identified metabolic fluxes revealed an increase in redox cofactor turnover, likely to combat increased production of reactive oxygen species. The metabolic changes are possibly a result of the age-associated decrease in surface area per cell volume. With metabolism being an important factor of the cellular phenotype, this work complements our recent mapping of the transcriptomic and proteomic changes towards a holistic description of the cellular phenotype during aging. eLife Sciences Publications, Ltd 2019-04-09 /pmc/articles/PMC6467564/ /pubmed/30963997 http://dx.doi.org/10.7554/eLife.41046 Text en © 2019, Leupold et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Leupold, Simeon
Hubmann, Georg
Litsios, Athanasios
Meinema, Anne C
Takhaveev, Vakil
Papagiannakis, Alexandros
Niebel, Bastian
Janssens, Georges
Siegel, David
Heinemann, Matthias
Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
title Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
title_full Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
title_fullStr Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
title_full_unstemmed Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
title_short Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
title_sort saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467564/
https://www.ncbi.nlm.nih.gov/pubmed/30963997
http://dx.doi.org/10.7554/eLife.41046
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