Cargando…
Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation?
OBJECTIVE: To evaluate whether methylene blue (MB) could minimize the effects of ischemia-reperfusion injury in the nonischemic lung on a lung transplantation rodent model. METHODS: Forty female Sprague-Dawley rats were divided into 20 donors and 20 recipients. The 20 recipient rats were divided int...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Pneumologia e Tisiologia
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467587/ https://www.ncbi.nlm.nih.gov/pubmed/30517338 http://dx.doi.org/10.1590/S1806-37562017000000172 |
_version_ | 1783411290298908672 |
---|---|
author | Abreu, Marcus da Matta de Almeida, Francine Maria dos Santos, Kelli Borges de Assis, Emílio Augusto Campos Pereira Hamada, Rafael Kenji Fonseca Jatene, Fabio Biscegli Pêgo-Fernandes, Paulo Manuel Pazetti, Rogerio |
author_facet | Abreu, Marcus da Matta de Almeida, Francine Maria dos Santos, Kelli Borges de Assis, Emílio Augusto Campos Pereira Hamada, Rafael Kenji Fonseca Jatene, Fabio Biscegli Pêgo-Fernandes, Paulo Manuel Pazetti, Rogerio |
author_sort | Abreu, Marcus da Matta |
collection | PubMed |
description | OBJECTIVE: To evaluate whether methylene blue (MB) could minimize the effects of ischemia-reperfusion injury in the nonischemic lung on a lung transplantation rodent model. METHODS: Forty female Sprague-Dawley rats were divided into 20 donors and 20 recipients. The 20 recipient rats were divided into two groups (n = 10) according to the treatment (0.9% saline vs. 1% MB solutions). All animals underwent unilateral lung transplantation. Recipients received 2 mL of saline or MB intraperitoneally prior to transplantation. After 2 h of reperfusion, the animals were euthanized and histopathological and immunohistochemical analyses were performed in the nonischemic lung. RESULTS: There was a significant decrease in inflammation-neutrophil count and intercellular adhesion molecule-1 (ICAM-1) expression in lung parenchyma were higher in the saline group in comparison with the MB group-and in apoptosis-caspase-3 expression was higher in the saline group and Bcl-2 expression was higher in MB group. CONCLUSIONS: MB is an effective drug for the protection of nonischemic lungs against inflammation and apoptosis following unilateral lung transplantation in rats. |
format | Online Article Text |
id | pubmed-6467587 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Sociedade Brasileira de Pneumologia e Tisiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-64675872019-05-01 Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? Abreu, Marcus da Matta de Almeida, Francine Maria dos Santos, Kelli Borges de Assis, Emílio Augusto Campos Pereira Hamada, Rafael Kenji Fonseca Jatene, Fabio Biscegli Pêgo-Fernandes, Paulo Manuel Pazetti, Rogerio J Bras Pneumol Artigo Original OBJECTIVE: To evaluate whether methylene blue (MB) could minimize the effects of ischemia-reperfusion injury in the nonischemic lung on a lung transplantation rodent model. METHODS: Forty female Sprague-Dawley rats were divided into 20 donors and 20 recipients. The 20 recipient rats were divided into two groups (n = 10) according to the treatment (0.9% saline vs. 1% MB solutions). All animals underwent unilateral lung transplantation. Recipients received 2 mL of saline or MB intraperitoneally prior to transplantation. After 2 h of reperfusion, the animals were euthanized and histopathological and immunohistochemical analyses were performed in the nonischemic lung. RESULTS: There was a significant decrease in inflammation-neutrophil count and intercellular adhesion molecule-1 (ICAM-1) expression in lung parenchyma were higher in the saline group in comparison with the MB group-and in apoptosis-caspase-3 expression was higher in the saline group and Bcl-2 expression was higher in MB group. CONCLUSIONS: MB is an effective drug for the protection of nonischemic lungs against inflammation and apoptosis following unilateral lung transplantation in rats. Sociedade Brasileira de Pneumologia e Tisiologia 2018 /pmc/articles/PMC6467587/ /pubmed/30517338 http://dx.doi.org/10.1590/S1806-37562017000000172 Text en © 2018 Sociedade Brasileira de Pneumologia e Tisiologia https://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Artigo Original Abreu, Marcus da Matta de Almeida, Francine Maria dos Santos, Kelli Borges de Assis, Emílio Augusto Campos Pereira Hamada, Rafael Kenji Fonseca Jatene, Fabio Biscegli Pêgo-Fernandes, Paulo Manuel Pazetti, Rogerio Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? |
title | Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? |
title_full | Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? |
title_fullStr | Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? |
title_full_unstemmed | Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? |
title_short | Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? |
title_sort | does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation? |
topic | Artigo Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467587/ https://www.ncbi.nlm.nih.gov/pubmed/30517338 http://dx.doi.org/10.1590/S1806-37562017000000172 |
work_keys_str_mv | AT abreumarcusdamatta doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation AT dealmeidafrancinemaria doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation AT dossantoskelliborges doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation AT deassisemilioaugustocampospereira doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation AT hamadarafaelkenjifonseca doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation AT jatenefabiobiscegli doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation AT pegofernandespaulomanuel doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation AT pazettirogerio doesmethyleneblueattenuateinflammationinnonischemiclungsafterlungtransplantation |