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Mechanistic characterization of the DEAD-box RNA helicase Ded1 from yeast as revealed by a novel technique using single-molecule magnetic tweezers

DEAD-box helicases are involved in all steps of RNA metabolism. They are ATP-dependent RNA binding proteins and RNA-dependent ATPases. They can displace short duplexes, but they lack processivity. Their mechanism and functioning are not clearly understood; classical or bulk biochemical assays are no...

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Detalles Bibliográficos
Autores principales:	Raj, Saurabh, Bagchi, Debjani, Orero, Jessica Valle, Banroques, Josette, Tanner, N Kyle, Croquette, Vincent
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2019
Materias:	RNA and RNA-protein complexes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468243/ https://www.ncbi.nlm.nih.gov/pubmed/30993346 http://dx.doi.org/10.1093/nar/gkz057

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