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circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells
NF-κB-mediated inflammatory phenotypic switching of vascular smooth muscle cells (VSMCs) plays a central role in atherosclerosis and neointimal formation. However, little is known about the roles of circRNAs in the regulation of NF-κB signaling. Here, we identify the involvement of circ-Sirt1 that w...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468289/ https://www.ncbi.nlm.nih.gov/pubmed/30820544 http://dx.doi.org/10.1093/nar/gkz141 |
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author | Kong, Peng Yu, Yuan Wang, Lu Dou, Yong-Qing Zhang, Xu-Hui Cui, Yan Wang, Hai-Yue Yong, Yu-Tao Liu, Ya-Bin Hu, Hai-Juan Cui, Wei Sun, Shao-Guang Li, Bing-Hui Zhang, Fan Han, Mei |
author_facet | Kong, Peng Yu, Yuan Wang, Lu Dou, Yong-Qing Zhang, Xu-Hui Cui, Yan Wang, Hai-Yue Yong, Yu-Tao Liu, Ya-Bin Hu, Hai-Juan Cui, Wei Sun, Shao-Guang Li, Bing-Hui Zhang, Fan Han, Mei |
author_sort | Kong, Peng |
collection | PubMed |
description | NF-κB-mediated inflammatory phenotypic switching of vascular smooth muscle cells (VSMCs) plays a central role in atherosclerosis and neointimal formation. However, little is known about the roles of circRNAs in the regulation of NF-κB signaling. Here, we identify the involvement of circ-Sirt1 that was one of transcripts of SIRT1 host gene in VSMC inflammatory response and neointimal hyperplasia. First, in the cytoplasm, circ-Sirt1 directly interacts with and sequesters NF-κB p65 from nuclear translocation induced by TNF-α in a sequence-dependent manner. The inhibitory complex of circ-Sirt1-NF-κB p65 is not dependent on IκBα. Second, circ-Sirt1 binds to miR-132/212 that interferes with SIRT1 mRNA, and facilitates the expression of host gene SIRT1. Increased SIRT1 results in deacetylation and inactivation of the nuclear NF-κB p65. These findings illustrate that circ-Sirt1 is a novel non-coding RNA regulator of VSMC phenotype. |
format | Online Article Text |
id | pubmed-6468289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-64682892019-04-22 circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells Kong, Peng Yu, Yuan Wang, Lu Dou, Yong-Qing Zhang, Xu-Hui Cui, Yan Wang, Hai-Yue Yong, Yu-Tao Liu, Ya-Bin Hu, Hai-Juan Cui, Wei Sun, Shao-Guang Li, Bing-Hui Zhang, Fan Han, Mei Nucleic Acids Res Molecular Biology NF-κB-mediated inflammatory phenotypic switching of vascular smooth muscle cells (VSMCs) plays a central role in atherosclerosis and neointimal formation. However, little is known about the roles of circRNAs in the regulation of NF-κB signaling. Here, we identify the involvement of circ-Sirt1 that was one of transcripts of SIRT1 host gene in VSMC inflammatory response and neointimal hyperplasia. First, in the cytoplasm, circ-Sirt1 directly interacts with and sequesters NF-κB p65 from nuclear translocation induced by TNF-α in a sequence-dependent manner. The inhibitory complex of circ-Sirt1-NF-κB p65 is not dependent on IκBα. Second, circ-Sirt1 binds to miR-132/212 that interferes with SIRT1 mRNA, and facilitates the expression of host gene SIRT1. Increased SIRT1 results in deacetylation and inactivation of the nuclear NF-κB p65. These findings illustrate that circ-Sirt1 is a novel non-coding RNA regulator of VSMC phenotype. Oxford University Press 2019-04-23 2019-03-01 /pmc/articles/PMC6468289/ /pubmed/30820544 http://dx.doi.org/10.1093/nar/gkz141 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Biology Kong, Peng Yu, Yuan Wang, Lu Dou, Yong-Qing Zhang, Xu-Hui Cui, Yan Wang, Hai-Yue Yong, Yu-Tao Liu, Ya-Bin Hu, Hai-Juan Cui, Wei Sun, Shao-Guang Li, Bing-Hui Zhang, Fan Han, Mei circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells |
title | circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells |
title_full | circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells |
title_fullStr | circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells |
title_full_unstemmed | circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells |
title_short | circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells |
title_sort | circ-sirt1 controls nf-κb activation via sequence-specific interaction and enhancement of sirt1 expression by binding to mir-132/212 in vascular smooth muscle cells |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468289/ https://www.ncbi.nlm.nih.gov/pubmed/30820544 http://dx.doi.org/10.1093/nar/gkz141 |
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