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Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods
Access to community-based point-of-care, low-cost, and sensitive tuberculosis (TB) diagnostics remains an unmet need. Objective: The objective of this study was to combine principles in nanotechnology, TB biology, glycochemistry, and engineering, for the development of a nanoparticle-based colorimet...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468399/ https://www.ncbi.nlm.nih.gov/pubmed/30586842 http://dx.doi.org/10.3390/bios9010001 |
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author | Bhusal, Nirajan Shrestha, Sunaina Pote, Nisha Alocilja, Evangelyn C. |
author_facet | Bhusal, Nirajan Shrestha, Sunaina Pote, Nisha Alocilja, Evangelyn C. |
author_sort | Bhusal, Nirajan |
collection | PubMed |
description | Access to community-based point-of-care, low-cost, and sensitive tuberculosis (TB) diagnostics remains an unmet need. Objective: The objective of this study was to combine principles in nanotechnology, TB biology, glycochemistry, and engineering, for the development of a nanoparticle-based colorimetric biosensing assay (NCBA) to quickly and inexpensively detect acid-fast bacilli (AFB) in sputum samples. Methods: In NCBA, the isolation of AFB from sputum samples was accomplished through glycan-coated magnetic nanoparticles (GMNP) interacting with AFB and then using a simple magnet to separate the GMNP-AFB complex. Acid-fastness and cording properties of mycobacteria were utilized to provide visually observable red-stained clumps of bacteria that were surrounded by brown nanoparticles under a light microscope on prepared smears. The NCBA technique was compared against sputum smear microscopy (SSM) and Xpert MTB/RIF in 500 samples from patients that were suspected to have TB. Results: Statistical analysis showed that NCBA had sensitivity and specificity performances in perfect agreement with Xpert MTB/RIF as gold standard for all 500 samples. SSM had a sensitivity of 40% for the same samples. Conclusion: NCBA technique yielded full agreement in terms of sensitivity and specificity with the Xpert MTB/RIF in 500 samples. The method is completed in 10–20 min through a simple process at an estimated cost of $0.10 per test. Implementation of NCBA in rural communities would help to increase case finding and case notification, and would support programs against drug-resistance. Its use at the first point-of-contact by patients in the healthcare system would facilitate quick treatment in a single clinical encounter, thus supporting the global “End TB Strategy” by 2035. |
format | Online Article Text |
id | pubmed-6468399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64683992019-04-23 Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods Bhusal, Nirajan Shrestha, Sunaina Pote, Nisha Alocilja, Evangelyn C. Biosensors (Basel) Article Access to community-based point-of-care, low-cost, and sensitive tuberculosis (TB) diagnostics remains an unmet need. Objective: The objective of this study was to combine principles in nanotechnology, TB biology, glycochemistry, and engineering, for the development of a nanoparticle-based colorimetric biosensing assay (NCBA) to quickly and inexpensively detect acid-fast bacilli (AFB) in sputum samples. Methods: In NCBA, the isolation of AFB from sputum samples was accomplished through glycan-coated magnetic nanoparticles (GMNP) interacting with AFB and then using a simple magnet to separate the GMNP-AFB complex. Acid-fastness and cording properties of mycobacteria were utilized to provide visually observable red-stained clumps of bacteria that were surrounded by brown nanoparticles under a light microscope on prepared smears. The NCBA technique was compared against sputum smear microscopy (SSM) and Xpert MTB/RIF in 500 samples from patients that were suspected to have TB. Results: Statistical analysis showed that NCBA had sensitivity and specificity performances in perfect agreement with Xpert MTB/RIF as gold standard for all 500 samples. SSM had a sensitivity of 40% for the same samples. Conclusion: NCBA technique yielded full agreement in terms of sensitivity and specificity with the Xpert MTB/RIF in 500 samples. The method is completed in 10–20 min through a simple process at an estimated cost of $0.10 per test. Implementation of NCBA in rural communities would help to increase case finding and case notification, and would support programs against drug-resistance. Its use at the first point-of-contact by patients in the healthcare system would facilitate quick treatment in a single clinical encounter, thus supporting the global “End TB Strategy” by 2035. MDPI 2018-12-24 /pmc/articles/PMC6468399/ /pubmed/30586842 http://dx.doi.org/10.3390/bios9010001 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bhusal, Nirajan Shrestha, Sunaina Pote, Nisha Alocilja, Evangelyn C. Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods |
title | Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods |
title_full | Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods |
title_fullStr | Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods |
title_full_unstemmed | Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods |
title_short | Nanoparticle-Based Biosensing of Tuberculosis, an Affordable and Practical Alternative to Current Methods |
title_sort | nanoparticle-based biosensing of tuberculosis, an affordable and practical alternative to current methods |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468399/ https://www.ncbi.nlm.nih.gov/pubmed/30586842 http://dx.doi.org/10.3390/bios9010001 |
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