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SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance

Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current a...

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Autores principales: Butterworth, Adrian, Blues, Elizabeth, Williamson, Paul, Cardona, Milovan, Gray, Louise, Corrigan, Damion K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468421/
https://www.ncbi.nlm.nih.gov/pubmed/30736460
http://dx.doi.org/10.3390/bios9010022
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author Butterworth, Adrian
Blues, Elizabeth
Williamson, Paul
Cardona, Milovan
Gray, Louise
Corrigan, Damion K
author_facet Butterworth, Adrian
Blues, Elizabeth
Williamson, Paul
Cardona, Milovan
Gray, Louise
Corrigan, Damion K
author_sort Butterworth, Adrian
collection PubMed
description Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance.
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spelling pubmed-64684212019-04-23 SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance Butterworth, Adrian Blues, Elizabeth Williamson, Paul Cardona, Milovan Gray, Louise Corrigan, Damion K Biosensors (Basel) Article Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance. MDPI 2019-02-07 /pmc/articles/PMC6468421/ /pubmed/30736460 http://dx.doi.org/10.3390/bios9010022 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Butterworth, Adrian
Blues, Elizabeth
Williamson, Paul
Cardona, Milovan
Gray, Louise
Corrigan, Damion K
SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
title SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
title_full SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
title_fullStr SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
title_full_unstemmed SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
title_short SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
title_sort sam composition and electrode roughness affect performance of a dna biosensor for antibiotic resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468421/
https://www.ncbi.nlm.nih.gov/pubmed/30736460
http://dx.doi.org/10.3390/bios9010022
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