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SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468421/ https://www.ncbi.nlm.nih.gov/pubmed/30736460 http://dx.doi.org/10.3390/bios9010022 |
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author | Butterworth, Adrian Blues, Elizabeth Williamson, Paul Cardona, Milovan Gray, Louise Corrigan, Damion K |
author_facet | Butterworth, Adrian Blues, Elizabeth Williamson, Paul Cardona, Milovan Gray, Louise Corrigan, Damion K |
author_sort | Butterworth, Adrian |
collection | PubMed |
description | Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance. |
format | Online Article Text |
id | pubmed-6468421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64684212019-04-23 SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance Butterworth, Adrian Blues, Elizabeth Williamson, Paul Cardona, Milovan Gray, Louise Corrigan, Damion K Biosensors (Basel) Article Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance. MDPI 2019-02-07 /pmc/articles/PMC6468421/ /pubmed/30736460 http://dx.doi.org/10.3390/bios9010022 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Butterworth, Adrian Blues, Elizabeth Williamson, Paul Cardona, Milovan Gray, Louise Corrigan, Damion K SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
title | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
title_full | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
title_fullStr | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
title_full_unstemmed | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
title_short | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
title_sort | sam composition and electrode roughness affect performance of a dna biosensor for antibiotic resistance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468421/ https://www.ncbi.nlm.nih.gov/pubmed/30736460 http://dx.doi.org/10.3390/bios9010022 |
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