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Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing

Simultaneous measurement of different substances from a single sample is an emerging issue for achieving efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytica...

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Autores principales: Anfossi, Laura, Di Nardo, Fabio, Cavalera, Simone, Giovannoli, Cristina, Baggiani, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468474/
https://www.ncbi.nlm.nih.gov/pubmed/30587769
http://dx.doi.org/10.3390/bios9010002
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author Anfossi, Laura
Di Nardo, Fabio
Cavalera, Simone
Giovannoli, Cristina
Baggiani, Claudio
author_facet Anfossi, Laura
Di Nardo, Fabio
Cavalera, Simone
Giovannoli, Cristina
Baggiani, Claudio
author_sort Anfossi, Laura
collection PubMed
description Simultaneous measurement of different substances from a single sample is an emerging issue for achieving efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytical platforms, one of the incoming challenges for immunoassay is exact multiplexing. Lateral flow immunoassay (LFIA) is a leading immunoanalytical technique for onsite analysis, thanks to its simplicity, rapidity, and cost-effectiveness. Moreover, LFIA architecture is adaptable to multiplexing, and is therefore a possible answer to the pressing demand of multiplexing point-of-need analysis. This review presents an overview of diverse approaches for multiplex LFIA, with a special focus on strategies based on new types of magnetic, fluorescent, and colored labels.
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spelling pubmed-64684742019-04-23 Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing Anfossi, Laura Di Nardo, Fabio Cavalera, Simone Giovannoli, Cristina Baggiani, Claudio Biosensors (Basel) Review Simultaneous measurement of different substances from a single sample is an emerging issue for achieving efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytical platforms, one of the incoming challenges for immunoassay is exact multiplexing. Lateral flow immunoassay (LFIA) is a leading immunoanalytical technique for onsite analysis, thanks to its simplicity, rapidity, and cost-effectiveness. Moreover, LFIA architecture is adaptable to multiplexing, and is therefore a possible answer to the pressing demand of multiplexing point-of-need analysis. This review presents an overview of diverse approaches for multiplex LFIA, with a special focus on strategies based on new types of magnetic, fluorescent, and colored labels. MDPI 2018-12-26 /pmc/articles/PMC6468474/ /pubmed/30587769 http://dx.doi.org/10.3390/bios9010002 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Anfossi, Laura
Di Nardo, Fabio
Cavalera, Simone
Giovannoli, Cristina
Baggiani, Claudio
Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing
title Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing
title_full Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing
title_fullStr Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing
title_full_unstemmed Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing
title_short Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing
title_sort multiplex lateral flow immunoassay: an overview of strategies towards high-throughput point-of-need testing
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468474/
https://www.ncbi.nlm.nih.gov/pubmed/30587769
http://dx.doi.org/10.3390/bios9010002
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