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Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types of tissue infections. For this, we collected 10–12 clinical S. aureus strains each from nasal colonization,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468552/ https://www.ncbi.nlm.nih.gov/pubmed/30823631 http://dx.doi.org/10.3390/toxins11030135 |
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author | Tuchscherr, Lorena Pöllath, Christine Siegmund, Anke Deinhardt-Emmer, Stefanie Hoerr, Verena Svensson, Carl-Magnus Thilo Figge, Marc Monecke, Stefan Löffler, Bettina |
author_facet | Tuchscherr, Lorena Pöllath, Christine Siegmund, Anke Deinhardt-Emmer, Stefanie Hoerr, Verena Svensson, Carl-Magnus Thilo Figge, Marc Monecke, Stefan Löffler, Bettina |
author_sort | Tuchscherr, Lorena |
collection | PubMed |
description | Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types of tissue infections. For this, we collected 10–12 clinical S. aureus strains each from nasal colonization, and from patients with endoprosthesis infection, hematogenous osteomyelitis, and sepsis. All strains were characterized by genotypic analysis, and by the expression of virulence factors. The host–pathogen interaction was studied through several functional assays in osteoblast cultures. Additionally, selected strains were tested in a murine sepsis/osteomyelitis model. We did not find characteristic bacterial features for the defined infection types; rather, a wide range in all strain collections regarding cytotoxicity and invasiveness was observed. Interestingly, all strains were able to persist and to form small colony variants (SCVs). However, the low-cytotoxicity strains survived in higher numbers, and were less efficiently cleared by the host than the highly cytotoxic strains. In summary, our results indicate that not only destructive, but also low-cytotoxicity strains are able to induce infections. The low-cytotoxicity strains can successfully survive, and are less efficiently cleared from the host than the highly cytotoxic strains, which represent a source for chronic infections. The understanding of this interplay/evolution between the host and the pathogen during infection, with specific attention towards low-cytotoxicity isolates, will help to optimize treatment strategies for invasive and therapy-refractory infection courses. |
format | Online Article Text |
id | pubmed-6468552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64685522019-04-22 Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host Tuchscherr, Lorena Pöllath, Christine Siegmund, Anke Deinhardt-Emmer, Stefanie Hoerr, Verena Svensson, Carl-Magnus Thilo Figge, Marc Monecke, Stefan Löffler, Bettina Toxins (Basel) Article Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types of tissue infections. For this, we collected 10–12 clinical S. aureus strains each from nasal colonization, and from patients with endoprosthesis infection, hematogenous osteomyelitis, and sepsis. All strains were characterized by genotypic analysis, and by the expression of virulence factors. The host–pathogen interaction was studied through several functional assays in osteoblast cultures. Additionally, selected strains were tested in a murine sepsis/osteomyelitis model. We did not find characteristic bacterial features for the defined infection types; rather, a wide range in all strain collections regarding cytotoxicity and invasiveness was observed. Interestingly, all strains were able to persist and to form small colony variants (SCVs). However, the low-cytotoxicity strains survived in higher numbers, and were less efficiently cleared by the host than the highly cytotoxic strains. In summary, our results indicate that not only destructive, but also low-cytotoxicity strains are able to induce infections. The low-cytotoxicity strains can successfully survive, and are less efficiently cleared from the host than the highly cytotoxic strains, which represent a source for chronic infections. The understanding of this interplay/evolution between the host and the pathogen during infection, with specific attention towards low-cytotoxicity isolates, will help to optimize treatment strategies for invasive and therapy-refractory infection courses. MDPI 2019-03-01 /pmc/articles/PMC6468552/ /pubmed/30823631 http://dx.doi.org/10.3390/toxins11030135 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tuchscherr, Lorena Pöllath, Christine Siegmund, Anke Deinhardt-Emmer, Stefanie Hoerr, Verena Svensson, Carl-Magnus Thilo Figge, Marc Monecke, Stefan Löffler, Bettina Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host |
title | Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host |
title_full | Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host |
title_fullStr | Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host |
title_full_unstemmed | Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host |
title_short | Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host |
title_sort | clinical s. aureus isolates vary in their virulence to promote adaptation to the host |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468552/ https://www.ncbi.nlm.nih.gov/pubmed/30823631 http://dx.doi.org/10.3390/toxins11030135 |
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