Cargando…

Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host

Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types of tissue infections. For this, we collected 10–12 clinical S. aureus strains each from nasal colonization,...

Descripción completa

Detalles Bibliográficos
Autores principales: Tuchscherr, Lorena, Pöllath, Christine, Siegmund, Anke, Deinhardt-Emmer, Stefanie, Hoerr, Verena, Svensson, Carl-Magnus, Thilo Figge, Marc, Monecke, Stefan, Löffler, Bettina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468552/
https://www.ncbi.nlm.nih.gov/pubmed/30823631
http://dx.doi.org/10.3390/toxins11030135
_version_ 1783411459874619392
author Tuchscherr, Lorena
Pöllath, Christine
Siegmund, Anke
Deinhardt-Emmer, Stefanie
Hoerr, Verena
Svensson, Carl-Magnus
Thilo Figge, Marc
Monecke, Stefan
Löffler, Bettina
author_facet Tuchscherr, Lorena
Pöllath, Christine
Siegmund, Anke
Deinhardt-Emmer, Stefanie
Hoerr, Verena
Svensson, Carl-Magnus
Thilo Figge, Marc
Monecke, Stefan
Löffler, Bettina
author_sort Tuchscherr, Lorena
collection PubMed
description Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types of tissue infections. For this, we collected 10–12 clinical S. aureus strains each from nasal colonization, and from patients with endoprosthesis infection, hematogenous osteomyelitis, and sepsis. All strains were characterized by genotypic analysis, and by the expression of virulence factors. The host–pathogen interaction was studied through several functional assays in osteoblast cultures. Additionally, selected strains were tested in a murine sepsis/osteomyelitis model. We did not find characteristic bacterial features for the defined infection types; rather, a wide range in all strain collections regarding cytotoxicity and invasiveness was observed. Interestingly, all strains were able to persist and to form small colony variants (SCVs). However, the low-cytotoxicity strains survived in higher numbers, and were less efficiently cleared by the host than the highly cytotoxic strains. In summary, our results indicate that not only destructive, but also low-cytotoxicity strains are able to induce infections. The low-cytotoxicity strains can successfully survive, and are less efficiently cleared from the host than the highly cytotoxic strains, which represent a source for chronic infections. The understanding of this interplay/evolution between the host and the pathogen during infection, with specific attention towards low-cytotoxicity isolates, will help to optimize treatment strategies for invasive and therapy-refractory infection courses.
format Online
Article
Text
id pubmed-6468552
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64685522019-04-22 Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host Tuchscherr, Lorena Pöllath, Christine Siegmund, Anke Deinhardt-Emmer, Stefanie Hoerr, Verena Svensson, Carl-Magnus Thilo Figge, Marc Monecke, Stefan Löffler, Bettina Toxins (Basel) Article Staphylococcus aureus colonizes epithelial surfaces, but it can also cause severe infections. The aim of this work was to investigate whether bacterial virulence correlates with defined types of tissue infections. For this, we collected 10–12 clinical S. aureus strains each from nasal colonization, and from patients with endoprosthesis infection, hematogenous osteomyelitis, and sepsis. All strains were characterized by genotypic analysis, and by the expression of virulence factors. The host–pathogen interaction was studied through several functional assays in osteoblast cultures. Additionally, selected strains were tested in a murine sepsis/osteomyelitis model. We did not find characteristic bacterial features for the defined infection types; rather, a wide range in all strain collections regarding cytotoxicity and invasiveness was observed. Interestingly, all strains were able to persist and to form small colony variants (SCVs). However, the low-cytotoxicity strains survived in higher numbers, and were less efficiently cleared by the host than the highly cytotoxic strains. In summary, our results indicate that not only destructive, but also low-cytotoxicity strains are able to induce infections. The low-cytotoxicity strains can successfully survive, and are less efficiently cleared from the host than the highly cytotoxic strains, which represent a source for chronic infections. The understanding of this interplay/evolution between the host and the pathogen during infection, with specific attention towards low-cytotoxicity isolates, will help to optimize treatment strategies for invasive and therapy-refractory infection courses. MDPI 2019-03-01 /pmc/articles/PMC6468552/ /pubmed/30823631 http://dx.doi.org/10.3390/toxins11030135 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tuchscherr, Lorena
Pöllath, Christine
Siegmund, Anke
Deinhardt-Emmer, Stefanie
Hoerr, Verena
Svensson, Carl-Magnus
Thilo Figge, Marc
Monecke, Stefan
Löffler, Bettina
Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
title Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
title_full Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
title_fullStr Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
title_full_unstemmed Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
title_short Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
title_sort clinical s. aureus isolates vary in their virulence to promote adaptation to the host
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468552/
https://www.ncbi.nlm.nih.gov/pubmed/30823631
http://dx.doi.org/10.3390/toxins11030135
work_keys_str_mv AT tuchscherrlorena clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT pollathchristine clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT siegmundanke clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT deinhardtemmerstefanie clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT hoerrverena clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT svenssoncarlmagnus clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT thilofiggemarc clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT moneckestefan clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost
AT lofflerbettina clinicalsaureusisolatesvaryintheirvirulencetopromoteadaptationtothehost