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Pannexin 2 Localizes at ER-Mitochondria Contact Sites

Endomembrane specialization allows functional compartmentalization but imposes physical constraints to information flow within the cell. However, the evolution of an endomembrane system was associated with the emergence of contact sites facilitating communication between membrane-bound organelles. C...

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Detalles Bibliográficos
Autores principales: Le Vasseur, Maxence, Chen, Vincent C., Huang, Kate, Vogl, Wayne A., Naus, Christian C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468579/
https://www.ncbi.nlm.nih.gov/pubmed/30862038
http://dx.doi.org/10.3390/cancers11030343
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author Le Vasseur, Maxence
Chen, Vincent C.
Huang, Kate
Vogl, Wayne A.
Naus, Christian C.
author_facet Le Vasseur, Maxence
Chen, Vincent C.
Huang, Kate
Vogl, Wayne A.
Naus, Christian C.
author_sort Le Vasseur, Maxence
collection PubMed
description Endomembrane specialization allows functional compartmentalization but imposes physical constraints to information flow within the cell. However, the evolution of an endomembrane system was associated with the emergence of contact sites facilitating communication between membrane-bound organelles. Contact sites between the endoplasmic reticulum (ER) and mitochondria are highly conserved in terms of their morphological features but show surprising molecular diversity within and across eukaryote species. ER-mitochondria contact sites are thought to regulate key processes in oncogenesis but their molecular composition remains poorly characterized in mammalian cells. In this study, we investigate the localization of pannexin 2 (Panx2), a membrane channel protein showing tumor-suppressing properties in cancer cells. Using a combination of subcellular fractionation, particle tracking in live-cell, and immunogold electron microscopy, we show that Panx2 localizes at ER-mitochondria contact sites in mammalian cells and sensitizes cells to apoptotic stimuli.
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spelling pubmed-64685792019-04-24 Pannexin 2 Localizes at ER-Mitochondria Contact Sites Le Vasseur, Maxence Chen, Vincent C. Huang, Kate Vogl, Wayne A. Naus, Christian C. Cancers (Basel) Article Endomembrane specialization allows functional compartmentalization but imposes physical constraints to information flow within the cell. However, the evolution of an endomembrane system was associated with the emergence of contact sites facilitating communication between membrane-bound organelles. Contact sites between the endoplasmic reticulum (ER) and mitochondria are highly conserved in terms of their morphological features but show surprising molecular diversity within and across eukaryote species. ER-mitochondria contact sites are thought to regulate key processes in oncogenesis but their molecular composition remains poorly characterized in mammalian cells. In this study, we investigate the localization of pannexin 2 (Panx2), a membrane channel protein showing tumor-suppressing properties in cancer cells. Using a combination of subcellular fractionation, particle tracking in live-cell, and immunogold electron microscopy, we show that Panx2 localizes at ER-mitochondria contact sites in mammalian cells and sensitizes cells to apoptotic stimuli. MDPI 2019-03-11 /pmc/articles/PMC6468579/ /pubmed/30862038 http://dx.doi.org/10.3390/cancers11030343 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Le Vasseur, Maxence
Chen, Vincent C.
Huang, Kate
Vogl, Wayne A.
Naus, Christian C.
Pannexin 2 Localizes at ER-Mitochondria Contact Sites
title Pannexin 2 Localizes at ER-Mitochondria Contact Sites
title_full Pannexin 2 Localizes at ER-Mitochondria Contact Sites
title_fullStr Pannexin 2 Localizes at ER-Mitochondria Contact Sites
title_full_unstemmed Pannexin 2 Localizes at ER-Mitochondria Contact Sites
title_short Pannexin 2 Localizes at ER-Mitochondria Contact Sites
title_sort pannexin 2 localizes at er-mitochondria contact sites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468579/
https://www.ncbi.nlm.nih.gov/pubmed/30862038
http://dx.doi.org/10.3390/cancers11030343
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