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Metabolic Analysis of Four Cultivars of Liriope platyphylla

Liriope platyphylla (Liliaceae), a medical plant distributed mainly in China, Taiwan, and Korea, has been used traditionally for the treatment of cough, sputum, asthma, and neurodegenerative diseases. The present study involved the metabolic profiling of this plant and reports spicatoside A accumula...

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Autores principales: Park, Chang Ha, Morgan, Abubaker Mohammed Awad, Park, Byung Bae, Lee, Sook Young, Lee, Sanghyun, Kim, Jae Kwang, Park, Sang Un
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468586/
https://www.ncbi.nlm.nih.gov/pubmed/30917595
http://dx.doi.org/10.3390/metabo9030059
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author Park, Chang Ha
Morgan, Abubaker Mohammed Awad
Park, Byung Bae
Lee, Sook Young
Lee, Sanghyun
Kim, Jae Kwang
Park, Sang Un
author_facet Park, Chang Ha
Morgan, Abubaker Mohammed Awad
Park, Byung Bae
Lee, Sook Young
Lee, Sanghyun
Kim, Jae Kwang
Park, Sang Un
author_sort Park, Chang Ha
collection PubMed
description Liriope platyphylla (Liliaceae), a medical plant distributed mainly in China, Taiwan, and Korea, has been used traditionally for the treatment of cough, sputum, asthma, and neurodegenerative diseases. The present study involved the metabolic profiling of this plant and reports spicatoside A accumulation in four different varieties of L. platyphylla (Cheongyangjaerae, Seongsoo, Cheongsim, and Liriope Tuber No. 1) using HPLC and gas chromatography time-of-flight mass spectrometry (GC–TOFMS). A total of 47 metabolites were detected in the different cultivars using GC–TOFMS-based metabolic profiling. The resulting data were subjected to principal component analysis (PCA) for determining the whole experimental variation, and the different cultivars were separated by score plots. Furthermore, hierarchical clustering, Pearson’s correlation, and partial least-squares discriminant analyses (PLS-DA) were subsequently performed to determine significant differences in the various metabolites of the cultivars. The HPLC data revealed that the presence of spicatoside A was detected in all four cultivars, with the amount of spicatoside A varying among them. Among the cultivars, Liriope Tuber No. 1 contained the highest amount of spicatoside A (1.83 ± 0.13 mg/g dry weight), followed by Cheongyangjaerae (1.25 ± 0.01 mg/g dry weight), Cheongsim (1.09 ± 0.04 mg/g dry weight), and Seongsoo (1.01 ± 0.02 mg/g dry weight). The identification of spicatoside A was confirmed by comparing the retention time of the sample with the retention time of the standard. Moreover, the Cheongsim cultivar contained higher levels of phenolic compounds—including vanillic acid, quinic acid, gallic acid, chlorogenic acid, caffeic acid, and benzoic acid—than those of the other two cultivars. On the other hand, the levels of amino acids were higher in the Seongsoo cultivar. Therefore, this study may help breeders produce new varieties with improved nutraceutical and nutritional qualities.
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spelling pubmed-64685862019-04-22 Metabolic Analysis of Four Cultivars of Liriope platyphylla Park, Chang Ha Morgan, Abubaker Mohammed Awad Park, Byung Bae Lee, Sook Young Lee, Sanghyun Kim, Jae Kwang Park, Sang Un Metabolites Article Liriope platyphylla (Liliaceae), a medical plant distributed mainly in China, Taiwan, and Korea, has been used traditionally for the treatment of cough, sputum, asthma, and neurodegenerative diseases. The present study involved the metabolic profiling of this plant and reports spicatoside A accumulation in four different varieties of L. platyphylla (Cheongyangjaerae, Seongsoo, Cheongsim, and Liriope Tuber No. 1) using HPLC and gas chromatography time-of-flight mass spectrometry (GC–TOFMS). A total of 47 metabolites were detected in the different cultivars using GC–TOFMS-based metabolic profiling. The resulting data were subjected to principal component analysis (PCA) for determining the whole experimental variation, and the different cultivars were separated by score plots. Furthermore, hierarchical clustering, Pearson’s correlation, and partial least-squares discriminant analyses (PLS-DA) were subsequently performed to determine significant differences in the various metabolites of the cultivars. The HPLC data revealed that the presence of spicatoside A was detected in all four cultivars, with the amount of spicatoside A varying among them. Among the cultivars, Liriope Tuber No. 1 contained the highest amount of spicatoside A (1.83 ± 0.13 mg/g dry weight), followed by Cheongyangjaerae (1.25 ± 0.01 mg/g dry weight), Cheongsim (1.09 ± 0.04 mg/g dry weight), and Seongsoo (1.01 ± 0.02 mg/g dry weight). The identification of spicatoside A was confirmed by comparing the retention time of the sample with the retention time of the standard. Moreover, the Cheongsim cultivar contained higher levels of phenolic compounds—including vanillic acid, quinic acid, gallic acid, chlorogenic acid, caffeic acid, and benzoic acid—than those of the other two cultivars. On the other hand, the levels of amino acids were higher in the Seongsoo cultivar. Therefore, this study may help breeders produce new varieties with improved nutraceutical and nutritional qualities. MDPI 2019-03-26 /pmc/articles/PMC6468586/ /pubmed/30917595 http://dx.doi.org/10.3390/metabo9030059 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Chang Ha
Morgan, Abubaker Mohammed Awad
Park, Byung Bae
Lee, Sook Young
Lee, Sanghyun
Kim, Jae Kwang
Park, Sang Un
Metabolic Analysis of Four Cultivars of Liriope platyphylla
title Metabolic Analysis of Four Cultivars of Liriope platyphylla
title_full Metabolic Analysis of Four Cultivars of Liriope platyphylla
title_fullStr Metabolic Analysis of Four Cultivars of Liriope platyphylla
title_full_unstemmed Metabolic Analysis of Four Cultivars of Liriope platyphylla
title_short Metabolic Analysis of Four Cultivars of Liriope platyphylla
title_sort metabolic analysis of four cultivars of liriope platyphylla
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468586/
https://www.ncbi.nlm.nih.gov/pubmed/30917595
http://dx.doi.org/10.3390/metabo9030059
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