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An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells

Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of gl...

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Autores principales: Herzog, Katharina, IJlst, Lodewijk, van Cruchten, Arno G., van Roermund, Carlo W.T., Kulik, Wim, Wanders, Ronald J. A., Waterham, Hans R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468779/
https://www.ncbi.nlm.nih.gov/pubmed/30841653
http://dx.doi.org/10.3390/metabo9030045
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author Herzog, Katharina
IJlst, Lodewijk
van Cruchten, Arno G.
van Roermund, Carlo W.T.
Kulik, Wim
Wanders, Ronald J. A.
Waterham, Hans R.
author_facet Herzog, Katharina
IJlst, Lodewijk
van Cruchten, Arno G.
van Roermund, Carlo W.T.
Kulik, Wim
Wanders, Ronald J. A.
Waterham, Hans R.
author_sort Herzog, Katharina
collection PubMed
description Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).
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spelling pubmed-64687792019-04-22 An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells Herzog, Katharina IJlst, Lodewijk van Cruchten, Arno G. van Roermund, Carlo W.T. Kulik, Wim Wanders, Ronald J. A. Waterham, Hans R. Metabolites Protocol Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). MDPI 2019-03-05 /pmc/articles/PMC6468779/ /pubmed/30841653 http://dx.doi.org/10.3390/metabo9030045 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Herzog, Katharina
IJlst, Lodewijk
van Cruchten, Arno G.
van Roermund, Carlo W.T.
Kulik, Wim
Wanders, Ronald J. A.
Waterham, Hans R.
An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
title An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
title_full An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
title_fullStr An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
title_full_unstemmed An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
title_short An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
title_sort uplc-ms/ms assay to measure glutathione as marker for oxidative stress in cultured cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468779/
https://www.ncbi.nlm.nih.gov/pubmed/30841653
http://dx.doi.org/10.3390/metabo9030045
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