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An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of gl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468779/ https://www.ncbi.nlm.nih.gov/pubmed/30841653 http://dx.doi.org/10.3390/metabo9030045 |
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author | Herzog, Katharina IJlst, Lodewijk van Cruchten, Arno G. van Roermund, Carlo W.T. Kulik, Wim Wanders, Ronald J. A. Waterham, Hans R. |
author_facet | Herzog, Katharina IJlst, Lodewijk van Cruchten, Arno G. van Roermund, Carlo W.T. Kulik, Wim Wanders, Ronald J. A. Waterham, Hans R. |
author_sort | Herzog, Katharina |
collection | PubMed |
description | Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). |
format | Online Article Text |
id | pubmed-6468779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64687792019-04-22 An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells Herzog, Katharina IJlst, Lodewijk van Cruchten, Arno G. van Roermund, Carlo W.T. Kulik, Wim Wanders, Ronald J. A. Waterham, Hans R. Metabolites Protocol Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). MDPI 2019-03-05 /pmc/articles/PMC6468779/ /pubmed/30841653 http://dx.doi.org/10.3390/metabo9030045 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Herzog, Katharina IJlst, Lodewijk van Cruchten, Arno G. van Roermund, Carlo W.T. Kulik, Wim Wanders, Ronald J. A. Waterham, Hans R. An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells |
title | An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells |
title_full | An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells |
title_fullStr | An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells |
title_full_unstemmed | An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells |
title_short | An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells |
title_sort | uplc-ms/ms assay to measure glutathione as marker for oxidative stress in cultured cells |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468779/ https://www.ncbi.nlm.nih.gov/pubmed/30841653 http://dx.doi.org/10.3390/metabo9030045 |
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