Cargando…

Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs

Dioclea reflexa bioactive compounds have been shown to contain antioxidant properties. The extracts from the same plant are used in traditional medical practices to treat various diseases with impressive outcomes. In this study, ionic mobility in Saccharomyces cerevisiae cells in the presence of D....

Descripción completa

Detalles Bibliográficos
Autores principales: Arthur, Patrick Kobina, Yeboah, Anthony Boadi, Issah, Ibrahim, Balapangu, Srinivasan, Kwofie, Samuel K., Asimeng, Bernard O., Foster, E. Johan, Tiburu, Elvis K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468906/
https://www.ncbi.nlm.nih.gov/pubmed/30897802
http://dx.doi.org/10.3390/bios9010045
_version_ 1783411541105704960
author Arthur, Patrick Kobina
Yeboah, Anthony Boadi
Issah, Ibrahim
Balapangu, Srinivasan
Kwofie, Samuel K.
Asimeng, Bernard O.
Foster, E. Johan
Tiburu, Elvis K.
author_facet Arthur, Patrick Kobina
Yeboah, Anthony Boadi
Issah, Ibrahim
Balapangu, Srinivasan
Kwofie, Samuel K.
Asimeng, Bernard O.
Foster, E. Johan
Tiburu, Elvis K.
author_sort Arthur, Patrick Kobina
collection PubMed
description Dioclea reflexa bioactive compounds have been shown to contain antioxidant properties. The extracts from the same plant are used in traditional medical practices to treat various diseases with impressive outcomes. In this study, ionic mobility in Saccharomyces cerevisiae cells in the presence of D. reflexa seed extracts was monitored using electrochemical detection methods to link cell death to ionic imbalance. Cells treated with ethanol, methanol, and water extracts were studied using cyclic voltammetry and cell counting to correlate electrochemical behavior and cell viability, respectively. The results were compared with cells treated with pore-forming Amphotericin b (Amp b), as well as Fluconazole (Flu) and the antimicrobial drug Rifampicin (Rif). The D. reflexa seed water extract (SWE) revealed higher anodic peak current with 58% cell death. Seed methanol extract (SME) and seed ethanol extract (SEE) recorded 31% and 22% cell death, respectively. Among the three control drugs, Flu revealed the highest cell death of about 64%, whereas Amp b and Rif exhibited cell deaths of 35% and 16%, respectively, after 8 h of cell growth. It was observed that similar to SWE, there was an increase in the anodic peak current in the presence of different concentrations of Amp b, which also correlated with enhanced cell death. It was concluded from this observation that Amp b and SWE might follow similar mechanisms to inhibit cell growth. Thus, the individual bioactive compounds from the water extracts of D. reflexa seeds could further be purified and tested to validate their potential therapeutic application. The strategy to link electrochemical behavior to biochemical responses could be a simple, fast, and robust screening technique for new drug targets and to understand the mechanism of action of such drugs against disease models.
format Online
Article
Text
id pubmed-6468906
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64689062019-04-23 Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs Arthur, Patrick Kobina Yeboah, Anthony Boadi Issah, Ibrahim Balapangu, Srinivasan Kwofie, Samuel K. Asimeng, Bernard O. Foster, E. Johan Tiburu, Elvis K. Biosensors (Basel) Article Dioclea reflexa bioactive compounds have been shown to contain antioxidant properties. The extracts from the same plant are used in traditional medical practices to treat various diseases with impressive outcomes. In this study, ionic mobility in Saccharomyces cerevisiae cells in the presence of D. reflexa seed extracts was monitored using electrochemical detection methods to link cell death to ionic imbalance. Cells treated with ethanol, methanol, and water extracts were studied using cyclic voltammetry and cell counting to correlate electrochemical behavior and cell viability, respectively. The results were compared with cells treated with pore-forming Amphotericin b (Amp b), as well as Fluconazole (Flu) and the antimicrobial drug Rifampicin (Rif). The D. reflexa seed water extract (SWE) revealed higher anodic peak current with 58% cell death. Seed methanol extract (SME) and seed ethanol extract (SEE) recorded 31% and 22% cell death, respectively. Among the three control drugs, Flu revealed the highest cell death of about 64%, whereas Amp b and Rif exhibited cell deaths of 35% and 16%, respectively, after 8 h of cell growth. It was observed that similar to SWE, there was an increase in the anodic peak current in the presence of different concentrations of Amp b, which also correlated with enhanced cell death. It was concluded from this observation that Amp b and SWE might follow similar mechanisms to inhibit cell growth. Thus, the individual bioactive compounds from the water extracts of D. reflexa seeds could further be purified and tested to validate their potential therapeutic application. The strategy to link electrochemical behavior to biochemical responses could be a simple, fast, and robust screening technique for new drug targets and to understand the mechanism of action of such drugs against disease models. MDPI 2019-03-20 /pmc/articles/PMC6468906/ /pubmed/30897802 http://dx.doi.org/10.3390/bios9010045 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arthur, Patrick Kobina
Yeboah, Anthony Boadi
Issah, Ibrahim
Balapangu, Srinivasan
Kwofie, Samuel K.
Asimeng, Bernard O.
Foster, E. Johan
Tiburu, Elvis K.
Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs
title Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs
title_full Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs
title_fullStr Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs
title_full_unstemmed Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs
title_short Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs
title_sort electrochemical response of saccharomyces cerevisiae corresponds to cell viability upon exposure to dioclea reflexa seed extracts and antifungal drugs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468906/
https://www.ncbi.nlm.nih.gov/pubmed/30897802
http://dx.doi.org/10.3390/bios9010045
work_keys_str_mv AT arthurpatrickkobina electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs
AT yeboahanthonyboadi electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs
AT issahibrahim electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs
AT balapangusrinivasan electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs
AT kwofiesamuelk electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs
AT asimengbernardo electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs
AT fosterejohan electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs
AT tiburuelvisk electrochemicalresponseofsaccharomycescerevisiaecorrespondstocellviabilityuponexposuretodiocleareflexaseedextractsandantifungaldrugs