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Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure

Epithelial fusion establishes continuity between the separated flanks of epithelial sheets. Despite its importance in creating resilient barriers, the mechanisms that ensure stable continuity and preserve morphological and molecular symmetry upon fusion remain unclear. Using the segmented embryonic...

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Autores principales: Das Gupta, Piyal Taru, Narasimha, Maithreyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469929/
https://www.ncbi.nlm.nih.gov/pubmed/30995201
http://dx.doi.org/10.7554/eLife.41091
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author Das Gupta, Piyal Taru
Narasimha, Maithreyi
author_facet Das Gupta, Piyal Taru
Narasimha, Maithreyi
author_sort Das Gupta, Piyal Taru
collection PubMed
description Epithelial fusion establishes continuity between the separated flanks of epithelial sheets. Despite its importance in creating resilient barriers, the mechanisms that ensure stable continuity and preserve morphological and molecular symmetry upon fusion remain unclear. Using the segmented embryonic epidermis whose flanks fuse during Drosophila dorsal closure, we demonstrate that epidermal flanks modulate cell numbers and geometry of their fusing fronts to achieve fusion fidelity. While fusing flanks become more matched for both parameters before fusion, differences persisting at fusion are corrected by modulating fusing front width within each segment to ensure alignment of segment boundaries. We show that fusing cell interfaces are remodelled from en-face contacts at fusion to an interlocking arrangement after fusion, and demonstrate that changes in interface length and geometry are dependent on the spatiotemporal regulation of cytoskeletal tension and Bazooka/Par3. Our work uncovers genetically constrained and mechanically triggered adaptive mechanisms contributing to fusion fidelity and epithelial continuity.
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spelling pubmed-64699292019-04-19 Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure Das Gupta, Piyal Taru Narasimha, Maithreyi eLife Cell Biology Epithelial fusion establishes continuity between the separated flanks of epithelial sheets. Despite its importance in creating resilient barriers, the mechanisms that ensure stable continuity and preserve morphological and molecular symmetry upon fusion remain unclear. Using the segmented embryonic epidermis whose flanks fuse during Drosophila dorsal closure, we demonstrate that epidermal flanks modulate cell numbers and geometry of their fusing fronts to achieve fusion fidelity. While fusing flanks become more matched for both parameters before fusion, differences persisting at fusion are corrected by modulating fusing front width within each segment to ensure alignment of segment boundaries. We show that fusing cell interfaces are remodelled from en-face contacts at fusion to an interlocking arrangement after fusion, and demonstrate that changes in interface length and geometry are dependent on the spatiotemporal regulation of cytoskeletal tension and Bazooka/Par3. Our work uncovers genetically constrained and mechanically triggered adaptive mechanisms contributing to fusion fidelity and epithelial continuity. eLife Sciences Publications, Ltd 2019-04-17 /pmc/articles/PMC6469929/ /pubmed/30995201 http://dx.doi.org/10.7554/eLife.41091 Text en © 2019, Das Gupta and Narasimha http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Das Gupta, Piyal Taru
Narasimha, Maithreyi
Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure
title Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure
title_full Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure
title_fullStr Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure
title_full_unstemmed Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure
title_short Cytoskeletal tension and Bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure
title_sort cytoskeletal tension and bazooka tune interface geometry to ensure fusion fidelity and sheet integrity during dorsal closure
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469929/
https://www.ncbi.nlm.nih.gov/pubmed/30995201
http://dx.doi.org/10.7554/eLife.41091
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