Cargando…
Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples
Vacuum-assisted evaporative concentration (VEC) was successfully applied and validated for the enrichment of 590 organic substances from river water and wastewater. Different volumes of water samples (6 mL wastewater influent, 15 mL wastewater effluent, and 60 mL river water) were evaporated to 0.3 ...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470124/ https://www.ncbi.nlm.nih.gov/pubmed/30854597 http://dx.doi.org/10.1007/s00216-019-01696-3 |
_version_ | 1783411730852872192 |
---|---|
author | Mechelke, Jonas Longrée, Philipp Singer, Heinz Hollender, Juliane |
author_facet | Mechelke, Jonas Longrée, Philipp Singer, Heinz Hollender, Juliane |
author_sort | Mechelke, Jonas |
collection | PubMed |
description | Vacuum-assisted evaporative concentration (VEC) was successfully applied and validated for the enrichment of 590 organic substances from river water and wastewater. Different volumes of water samples (6 mL wastewater influent, 15 mL wastewater effluent, and 60 mL river water) were evaporated to 0.3 mL and finally adjusted to 0.4 mL. 0.1 mL of the concentrate were injected into a polar reversed-phase C18 liquid chromatography column coupled with electrospray ionization to high-resolution tandem mass spectrometry. Analyte recoveries were determined for VEC and compared against a mixed-bed multilayer solid-phase extraction (SPE). Both approaches performed equally well (≥ 70% recovery) for a vast number of analytes (n = 327), whereas certain substances were especially amenable to enrichment by either SPE (e.g., 4-chlorobenzophenone, logD(ow,pH7) 4) or VEC (e.g., TRIS, logD(ow,pH7) − 4.6). Overall, VEC was more suitable for the enrichment of polar analytes, albeit considerable signal suppression (up to 74% in river water) was observed for the VEC-enriched sample matrix. Nevertheless, VEC allowed for accurate and precise quantification down to the sub-nanogram per liter level and required no more than 60 mL of the sample, as demonstrated by its application to several environmental water matrices. By contrast, SPE is typically constrained by high sample volumes ranging from 100 mL (wastewater influent) to 1000 mL (river water). The developed VEC workflow not only requires low labor cost and minimum supervision but is also a rapid, convenient, and environmentally safe alternative to SPE and highly suitable for target and non-target analysis. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-01696-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6470124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-64701242019-05-03 Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples Mechelke, Jonas Longrée, Philipp Singer, Heinz Hollender, Juliane Anal Bioanal Chem Research Paper Vacuum-assisted evaporative concentration (VEC) was successfully applied and validated for the enrichment of 590 organic substances from river water and wastewater. Different volumes of water samples (6 mL wastewater influent, 15 mL wastewater effluent, and 60 mL river water) were evaporated to 0.3 mL and finally adjusted to 0.4 mL. 0.1 mL of the concentrate were injected into a polar reversed-phase C18 liquid chromatography column coupled with electrospray ionization to high-resolution tandem mass spectrometry. Analyte recoveries were determined for VEC and compared against a mixed-bed multilayer solid-phase extraction (SPE). Both approaches performed equally well (≥ 70% recovery) for a vast number of analytes (n = 327), whereas certain substances were especially amenable to enrichment by either SPE (e.g., 4-chlorobenzophenone, logD(ow,pH7) 4) or VEC (e.g., TRIS, logD(ow,pH7) − 4.6). Overall, VEC was more suitable for the enrichment of polar analytes, albeit considerable signal suppression (up to 74% in river water) was observed for the VEC-enriched sample matrix. Nevertheless, VEC allowed for accurate and precise quantification down to the sub-nanogram per liter level and required no more than 60 mL of the sample, as demonstrated by its application to several environmental water matrices. By contrast, SPE is typically constrained by high sample volumes ranging from 100 mL (wastewater influent) to 1000 mL (river water). The developed VEC workflow not only requires low labor cost and minimum supervision but is also a rapid, convenient, and environmentally safe alternative to SPE and highly suitable for target and non-target analysis. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-01696-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-03-11 2019 /pmc/articles/PMC6470124/ /pubmed/30854597 http://dx.doi.org/10.1007/s00216-019-01696-3 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Paper Mechelke, Jonas Longrée, Philipp Singer, Heinz Hollender, Juliane Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples |
title | Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples |
title_full | Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples |
title_fullStr | Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples |
title_full_unstemmed | Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples |
title_short | Vacuum-assisted evaporative concentration combined with LC-HRMS/MS for ultra-trace-level screening of organic micropollutants in environmental water samples |
title_sort | vacuum-assisted evaporative concentration combined with lc-hrms/ms for ultra-trace-level screening of organic micropollutants in environmental water samples |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470124/ https://www.ncbi.nlm.nih.gov/pubmed/30854597 http://dx.doi.org/10.1007/s00216-019-01696-3 |
work_keys_str_mv | AT mechelkejonas vacuumassistedevaporativeconcentrationcombinedwithlchrmsmsforultratracelevelscreeningoforganicmicropollutantsinenvironmentalwatersamples AT longreephilipp vacuumassistedevaporativeconcentrationcombinedwithlchrmsmsforultratracelevelscreeningoforganicmicropollutantsinenvironmentalwatersamples AT singerheinz vacuumassistedevaporativeconcentrationcombinedwithlchrmsmsforultratracelevelscreeningoforganicmicropollutantsinenvironmentalwatersamples AT hollenderjuliane vacuumassistedevaporativeconcentrationcombinedwithlchrmsmsforultratracelevelscreeningoforganicmicropollutantsinenvironmentalwatersamples |