Cargando…
UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related mortality worldwide. Recently, ubiquitin-conjugating enzyme E2C (UBE2C) has been reported to be overexpressed in human cancers and act as a potential oncogene. However, little is known about the functional roles of UBE2C in...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470407/ https://www.ncbi.nlm.nih.gov/pubmed/30914455 http://dx.doi.org/10.1042/BSR20182384 |
_version_ | 1783411789813252096 |
---|---|
author | Xiong, Yu Lu, Jing Fang, Qinliang Lu, Yuyan Xie, Chengrong Wu, Huita Yin, Zhenyu |
author_facet | Xiong, Yu Lu, Jing Fang, Qinliang Lu, Yuyan Xie, Chengrong Wu, Huita Yin, Zhenyu |
author_sort | Xiong, Yu |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related mortality worldwide. Recently, ubiquitin-conjugating enzyme E2C (UBE2C) has been reported to be overexpressed in human cancers and act as a potential oncogene. However, little is known about the functional roles of UBE2C in HCC progression. In the present study, analysis of UBE2C mRNA expression in The Cancer Genome Atlas (TCGA) dataset reveals that significantly higher UBE2C mRNA levels was found in HCC tissues and associated with higher HCC grade. Elevated UBE2C mRNA levels in HCC indicated worsened survival probabilities. Through performing loss-of-function assays, we demonstrated that knockdown of UBE2C expression obviously suppressed proliferation, migration, and invasion of HCC cells in vitro. Moreover, HCC cells with UBE2C knockdown showed higher sensitivity for the treatment of chemotherapeutic drug, including adriamycin (ADR) and 5-fluorouracil (5-FU). Silencing of UBE2C also increased the sensitivity of HCC cells to sorafenib, an approved treatment for patients with advanced-stage HCC. Our findings strongly suggest that UBE2C emerges as a marker for prognosis in HCC, and blocking UBE2C may be a novel strategy for HCC therapies. |
format | Online Article Text |
id | pubmed-6470407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64704072019-04-26 UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells Xiong, Yu Lu, Jing Fang, Qinliang Lu, Yuyan Xie, Chengrong Wu, Huita Yin, Zhenyu Biosci Rep Research Articles Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related mortality worldwide. Recently, ubiquitin-conjugating enzyme E2C (UBE2C) has been reported to be overexpressed in human cancers and act as a potential oncogene. However, little is known about the functional roles of UBE2C in HCC progression. In the present study, analysis of UBE2C mRNA expression in The Cancer Genome Atlas (TCGA) dataset reveals that significantly higher UBE2C mRNA levels was found in HCC tissues and associated with higher HCC grade. Elevated UBE2C mRNA levels in HCC indicated worsened survival probabilities. Through performing loss-of-function assays, we demonstrated that knockdown of UBE2C expression obviously suppressed proliferation, migration, and invasion of HCC cells in vitro. Moreover, HCC cells with UBE2C knockdown showed higher sensitivity for the treatment of chemotherapeutic drug, including adriamycin (ADR) and 5-fluorouracil (5-FU). Silencing of UBE2C also increased the sensitivity of HCC cells to sorafenib, an approved treatment for patients with advanced-stage HCC. Our findings strongly suggest that UBE2C emerges as a marker for prognosis in HCC, and blocking UBE2C may be a novel strategy for HCC therapies. Portland Press Ltd. 2019-04-17 /pmc/articles/PMC6470407/ /pubmed/30914455 http://dx.doi.org/10.1042/BSR20182384 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Xiong, Yu Lu, Jing Fang, Qinliang Lu, Yuyan Xie, Chengrong Wu, Huita Yin, Zhenyu UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells |
title | UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells |
title_full | UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells |
title_fullStr | UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells |
title_full_unstemmed | UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells |
title_short | UBE2C functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells |
title_sort | ube2c functions as a potential oncogene by enhancing cell proliferation, migration, invasion, and drug resistance in hepatocellular carcinoma cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470407/ https://www.ncbi.nlm.nih.gov/pubmed/30914455 http://dx.doi.org/10.1042/BSR20182384 |
work_keys_str_mv | AT xiongyu ube2cfunctionsasapotentialoncogenebyenhancingcellproliferationmigrationinvasionanddrugresistanceinhepatocellularcarcinomacells AT lujing ube2cfunctionsasapotentialoncogenebyenhancingcellproliferationmigrationinvasionanddrugresistanceinhepatocellularcarcinomacells AT fangqinliang ube2cfunctionsasapotentialoncogenebyenhancingcellproliferationmigrationinvasionanddrugresistanceinhepatocellularcarcinomacells AT luyuyan ube2cfunctionsasapotentialoncogenebyenhancingcellproliferationmigrationinvasionanddrugresistanceinhepatocellularcarcinomacells AT xiechengrong ube2cfunctionsasapotentialoncogenebyenhancingcellproliferationmigrationinvasionanddrugresistanceinhepatocellularcarcinomacells AT wuhuita ube2cfunctionsasapotentialoncogenebyenhancingcellproliferationmigrationinvasionanddrugresistanceinhepatocellularcarcinomacells AT yinzhenyu ube2cfunctionsasapotentialoncogenebyenhancingcellproliferationmigrationinvasionanddrugresistanceinhepatocellularcarcinomacells |