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Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon
One carbon (1C) metabolism nutrients influence epigenetic regulation and they are supplied by diet and synthesized by gut microbiota. We examined the association between dietary consumption of methyl donors (methionine, betaine and choline) and B vitamins (folate, B2, B6, and B12) and the community...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470629/ https://www.ncbi.nlm.nih.gov/pubmed/30871224 http://dx.doi.org/10.3390/nu11030613 |
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author | Gurwara, Shawn Ajami, Nadim J. Jang, Albert Hessel, Frances C. Chen, Liang Plew, Sarah Wang, Zhensheng Graham, David Y. Hair, Clark White, Donna L. Kramer, Jennifer Kourkoumpetis, Themistoklis Hoffman, Kristi Cole, Rhonda Hou, Jason Husain, Nisreen Jarbrink-Sehgal, Maria Hernaez, Ruben Kanwal, Fasiha Ketwaroo, Gyanprakash Shah, Rajesh Velez, Maria Natarajan, Yamini El-Serag, Hashem B. Petrosino, Joseph F. Jiao, Li |
author_facet | Gurwara, Shawn Ajami, Nadim J. Jang, Albert Hessel, Frances C. Chen, Liang Plew, Sarah Wang, Zhensheng Graham, David Y. Hair, Clark White, Donna L. Kramer, Jennifer Kourkoumpetis, Themistoklis Hoffman, Kristi Cole, Rhonda Hou, Jason Husain, Nisreen Jarbrink-Sehgal, Maria Hernaez, Ruben Kanwal, Fasiha Ketwaroo, Gyanprakash Shah, Rajesh Velez, Maria Natarajan, Yamini El-Serag, Hashem B. Petrosino, Joseph F. Jiao, Li |
author_sort | Gurwara, Shawn |
collection | PubMed |
description | One carbon (1C) metabolism nutrients influence epigenetic regulation and they are supplied by diet and synthesized by gut microbiota. We examined the association between dietary consumption of methyl donors (methionine, betaine and choline) and B vitamins (folate, B2, B6, and B12) and the community composition and structure of the colonic mucosa-associated gut microbiota determined by 16S rRNA gene sequencing in 97 colonic biopsies of 35 men. We used the food frequency questionnaire to assess daily consumption of nutrients, and the UPARSE and SILVA databases for operational taxonomic unit classification. The difference in bacterial diversity and taxonomic relative abundance were compared between low versus high consumption of these nutrients. False discover rate (FDR) adjusted p value < 0.05 indicated statistical significance. The bacterial richness and composition differed significantly by the consumption of folate and B vitamins (p < 0.001). Compared with higher consumption, a lower consumption of these nutrients was associated with a lower abundance of Akkermansia (folate), Roseburia (vitamin B2), and Faecalibacterium (vitamins B2, B6, and B12) but a higher abundance of Erysipelatoclostridium (vitamin B2) (FDR p values < 0.05). The community composition and structure of the colonic bacteria differed significantly by dietary consumption of folate and B vitamins. |
format | Online Article Text |
id | pubmed-6470629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64706292019-04-25 Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon Gurwara, Shawn Ajami, Nadim J. Jang, Albert Hessel, Frances C. Chen, Liang Plew, Sarah Wang, Zhensheng Graham, David Y. Hair, Clark White, Donna L. Kramer, Jennifer Kourkoumpetis, Themistoklis Hoffman, Kristi Cole, Rhonda Hou, Jason Husain, Nisreen Jarbrink-Sehgal, Maria Hernaez, Ruben Kanwal, Fasiha Ketwaroo, Gyanprakash Shah, Rajesh Velez, Maria Natarajan, Yamini El-Serag, Hashem B. Petrosino, Joseph F. Jiao, Li Nutrients Article One carbon (1C) metabolism nutrients influence epigenetic regulation and they are supplied by diet and synthesized by gut microbiota. We examined the association between dietary consumption of methyl donors (methionine, betaine and choline) and B vitamins (folate, B2, B6, and B12) and the community composition and structure of the colonic mucosa-associated gut microbiota determined by 16S rRNA gene sequencing in 97 colonic biopsies of 35 men. We used the food frequency questionnaire to assess daily consumption of nutrients, and the UPARSE and SILVA databases for operational taxonomic unit classification. The difference in bacterial diversity and taxonomic relative abundance were compared between low versus high consumption of these nutrients. False discover rate (FDR) adjusted p value < 0.05 indicated statistical significance. The bacterial richness and composition differed significantly by the consumption of folate and B vitamins (p < 0.001). Compared with higher consumption, a lower consumption of these nutrients was associated with a lower abundance of Akkermansia (folate), Roseburia (vitamin B2), and Faecalibacterium (vitamins B2, B6, and B12) but a higher abundance of Erysipelatoclostridium (vitamin B2) (FDR p values < 0.05). The community composition and structure of the colonic bacteria differed significantly by dietary consumption of folate and B vitamins. MDPI 2019-03-13 /pmc/articles/PMC6470629/ /pubmed/30871224 http://dx.doi.org/10.3390/nu11030613 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gurwara, Shawn Ajami, Nadim J. Jang, Albert Hessel, Frances C. Chen, Liang Plew, Sarah Wang, Zhensheng Graham, David Y. Hair, Clark White, Donna L. Kramer, Jennifer Kourkoumpetis, Themistoklis Hoffman, Kristi Cole, Rhonda Hou, Jason Husain, Nisreen Jarbrink-Sehgal, Maria Hernaez, Ruben Kanwal, Fasiha Ketwaroo, Gyanprakash Shah, Rajesh Velez, Maria Natarajan, Yamini El-Serag, Hashem B. Petrosino, Joseph F. Jiao, Li Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon |
title | Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon |
title_full | Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon |
title_fullStr | Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon |
title_full_unstemmed | Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon |
title_short | Dietary Nutrients Involved in One-Carbon Metabolism and Colonic Mucosa-Associated Gut Microbiome in Individuals with an Endoscopically Normal Colon |
title_sort | dietary nutrients involved in one-carbon metabolism and colonic mucosa-associated gut microbiome in individuals with an endoscopically normal colon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470629/ https://www.ncbi.nlm.nih.gov/pubmed/30871224 http://dx.doi.org/10.3390/nu11030613 |
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