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Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability
DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470866/ https://www.ncbi.nlm.nih.gov/pubmed/30901888 http://dx.doi.org/10.3390/ma12060949 |
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author | Xu, Xuemei Fang, Shiqi Zhuang, Yuan Wu, Shanshan Pan, Qingling Li, Longjie Wang, Xiaohui Sun, Xueqing Liu, Bifeng Wu, Yuzhou |
author_facet | Xu, Xuemei Fang, Shiqi Zhuang, Yuan Wu, Shanshan Pan, Qingling Li, Longjie Wang, Xiaohui Sun, Xueqing Liu, Bifeng Wu, Yuzhou |
author_sort | Xu, Xuemei |
collection | PubMed |
description | DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells, weak stability under physiological conditions, and endonuclease degradation. Herein, we demonstrate an effective approach to facilitate fast transfection of DNA nanostructures and enhance their stability by encapsulating DNA origami with a biocompatible cationic protein (cHSA) via electrostatic interaction. The coated DNA origami is found to be stable under physiological conditions. Moreover, the cHSA coating could significantly improve the cellular transfection efficiency of DNA origami, which is essential for biological applications. |
format | Online Article Text |
id | pubmed-6470866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64708662019-04-27 Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability Xu, Xuemei Fang, Shiqi Zhuang, Yuan Wu, Shanshan Pan, Qingling Li, Longjie Wang, Xiaohui Sun, Xueqing Liu, Bifeng Wu, Yuzhou Materials (Basel) Communication DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells, weak stability under physiological conditions, and endonuclease degradation. Herein, we demonstrate an effective approach to facilitate fast transfection of DNA nanostructures and enhance their stability by encapsulating DNA origami with a biocompatible cationic protein (cHSA) via electrostatic interaction. The coated DNA origami is found to be stable under physiological conditions. Moreover, the cHSA coating could significantly improve the cellular transfection efficiency of DNA origami, which is essential for biological applications. MDPI 2019-03-21 /pmc/articles/PMC6470866/ /pubmed/30901888 http://dx.doi.org/10.3390/ma12060949 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Xu, Xuemei Fang, Shiqi Zhuang, Yuan Wu, Shanshan Pan, Qingling Li, Longjie Wang, Xiaohui Sun, Xueqing Liu, Bifeng Wu, Yuzhou Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability |
title | Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability |
title_full | Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability |
title_fullStr | Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability |
title_full_unstemmed | Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability |
title_short | Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability |
title_sort | cationic albumin encapsulated dna origami for enhanced cellular transfection and stability |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470866/ https://www.ncbi.nlm.nih.gov/pubmed/30901888 http://dx.doi.org/10.3390/ma12060949 |
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