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Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation

In recent years, the use of botanical agents to prevent skin damage from solar ultraviolet (UV) irradiation has received considerable attention. Oenanthe javanica is known to exert anti-inflammatory and antioxidant activities. This study investigated photoprotective properties of an Oenanthe javanic...

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Autores principales: Her, Young, Shin, Bich-Na, Lee, Yun Lyul, Park, Joon Ha, Kim, Dae Won, Kim, Ki Seob, Kim, Hyunjung, Song, Minah, Kim, Jong-Dai, Won, Moo-Ho, Ahn, Ji Hyeon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470913/
https://www.ncbi.nlm.nih.gov/pubmed/30901885
http://dx.doi.org/10.3390/ijms20061435
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author Her, Young
Shin, Bich-Na
Lee, Yun Lyul
Park, Joon Ha
Kim, Dae Won
Kim, Ki Seob
Kim, Hyunjung
Song, Minah
Kim, Jong-Dai
Won, Moo-Ho
Ahn, Ji Hyeon
author_facet Her, Young
Shin, Bich-Na
Lee, Yun Lyul
Park, Joon Ha
Kim, Dae Won
Kim, Ki Seob
Kim, Hyunjung
Song, Minah
Kim, Jong-Dai
Won, Moo-Ho
Ahn, Ji Hyeon
author_sort Her, Young
collection PubMed
description In recent years, the use of botanical agents to prevent skin damage from solar ultraviolet (UV) irradiation has received considerable attention. Oenanthe javanica is known to exert anti-inflammatory and antioxidant activities. This study investigated photoprotective properties of an Oenanthe javanica extract (OJE) against UVB-induced skin damage in ICR mice. The extent of skin damage was evaluated in three groups: control mice with no UVB, UVB-exposed mice treated with vehicle (saline), and UVB-exposed mice treated with 1% extract. Photoprotective properties were assessed in the dorsal skin using hematoxylin and eosin staining, Masson trichrome staining, immunohistochemical staining, quantitative real-time polymerase chain reaction, and western blotting to analyze the epidermal thickness, collagen expression, and mRNA and protein levels of type I collagen, type III collagen, and interstitial collagenases, including matrix metalloproteinase (MMP)-1 and MMP-3. In addition, tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 protein levels were also assessed. In the UVB-exposed mice treated with extract, UV-induced epidermal damage was significantly ameliorated. In this group, productions of collagen types I and III were increased, and expressions of MMP-1 and MMP-3 were decreased. In addition, TNF-α and COX-2 expressions were reduced. Based on these findings, we conclude that OJE displays photoprotective effects against UVB-induced collagen disruption and inflammation and suggest that Oenanthe javanica can be used as a natural product for the treatment of photodamaged skin.
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spelling pubmed-64709132019-04-26 Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation Her, Young Shin, Bich-Na Lee, Yun Lyul Park, Joon Ha Kim, Dae Won Kim, Ki Seob Kim, Hyunjung Song, Minah Kim, Jong-Dai Won, Moo-Ho Ahn, Ji Hyeon Int J Mol Sci Article In recent years, the use of botanical agents to prevent skin damage from solar ultraviolet (UV) irradiation has received considerable attention. Oenanthe javanica is known to exert anti-inflammatory and antioxidant activities. This study investigated photoprotective properties of an Oenanthe javanica extract (OJE) against UVB-induced skin damage in ICR mice. The extent of skin damage was evaluated in three groups: control mice with no UVB, UVB-exposed mice treated with vehicle (saline), and UVB-exposed mice treated with 1% extract. Photoprotective properties were assessed in the dorsal skin using hematoxylin and eosin staining, Masson trichrome staining, immunohistochemical staining, quantitative real-time polymerase chain reaction, and western blotting to analyze the epidermal thickness, collagen expression, and mRNA and protein levels of type I collagen, type III collagen, and interstitial collagenases, including matrix metalloproteinase (MMP)-1 and MMP-3. In addition, tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 protein levels were also assessed. In the UVB-exposed mice treated with extract, UV-induced epidermal damage was significantly ameliorated. In this group, productions of collagen types I and III were increased, and expressions of MMP-1 and MMP-3 were decreased. In addition, TNF-α and COX-2 expressions were reduced. Based on these findings, we conclude that OJE displays photoprotective effects against UVB-induced collagen disruption and inflammation and suggest that Oenanthe javanica can be used as a natural product for the treatment of photodamaged skin. MDPI 2019-03-21 /pmc/articles/PMC6470913/ /pubmed/30901885 http://dx.doi.org/10.3390/ijms20061435 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Her, Young
Shin, Bich-Na
Lee, Yun Lyul
Park, Joon Ha
Kim, Dae Won
Kim, Ki Seob
Kim, Hyunjung
Song, Minah
Kim, Jong-Dai
Won, Moo-Ho
Ahn, Ji Hyeon
Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation
title Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation
title_full Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation
title_fullStr Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation
title_full_unstemmed Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation
title_short Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation
title_sort oenanthe javanica extract protects mouse skin from uvb radiation via attenuating collagen disruption and inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470913/
https://www.ncbi.nlm.nih.gov/pubmed/30901885
http://dx.doi.org/10.3390/ijms20061435
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