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Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis

Individual variation in animal venom has been linked to geographical location, feeding habit, season, size, and gender. Uniquely, cone snails possess the remarkable ability to change venom composition in response to predatory or defensive stimuli. To date, correlations between the venom gland transc...

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Autores principales: Jin, Ai-Hua, Dutertre, Sébastien, Dutt, Mriga, Lavergne, Vincent, Jones, Alun, Lewis, Richard J., Alewood, Paul F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471084/
https://www.ncbi.nlm.nih.gov/pubmed/30893765
http://dx.doi.org/10.3390/md17030177
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author Jin, Ai-Hua
Dutertre, Sébastien
Dutt, Mriga
Lavergne, Vincent
Jones, Alun
Lewis, Richard J.
Alewood, Paul F.
author_facet Jin, Ai-Hua
Dutertre, Sébastien
Dutt, Mriga
Lavergne, Vincent
Jones, Alun
Lewis, Richard J.
Alewood, Paul F.
author_sort Jin, Ai-Hua
collection PubMed
description Individual variation in animal venom has been linked to geographical location, feeding habit, season, size, and gender. Uniquely, cone snails possess the remarkable ability to change venom composition in response to predatory or defensive stimuli. To date, correlations between the venom gland transcriptome and proteome within and between individual cone snails have not been reported. In this study, we use 454 pyrosequencing and mass spectrometry to decipher the transcriptomes and proteomes of the venom gland and corresponding predation-evoked venom of two specimens of Conus imperialis. Transcriptomic analyses revealed 17 conotoxin gene superfamilies common to both animals, including 5 novel superfamilies and two novel cysteine frameworks. While highly expressed transcripts were common to both specimens, variation of moderately and weakly expressed precursor sequences was surprisingly diverse, with one specimen expressing two unique gene superfamilies and consistently producing more paralogs within each conotoxin gene superfamily. Using a quantitative labelling method, conotoxin variability was compared quantitatively, with highly expressed peptides showing a strong correlation between transcription and translation, whereas peptides expressed at lower levels showed a poor correlation. These results suggest that major transcripts are subject to stabilizing selection, while minor transcripts are subject to diversifying selection.
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spelling pubmed-64710842019-04-27 Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis Jin, Ai-Hua Dutertre, Sébastien Dutt, Mriga Lavergne, Vincent Jones, Alun Lewis, Richard J. Alewood, Paul F. Mar Drugs Article Individual variation in animal venom has been linked to geographical location, feeding habit, season, size, and gender. Uniquely, cone snails possess the remarkable ability to change venom composition in response to predatory or defensive stimuli. To date, correlations between the venom gland transcriptome and proteome within and between individual cone snails have not been reported. In this study, we use 454 pyrosequencing and mass spectrometry to decipher the transcriptomes and proteomes of the venom gland and corresponding predation-evoked venom of two specimens of Conus imperialis. Transcriptomic analyses revealed 17 conotoxin gene superfamilies common to both animals, including 5 novel superfamilies and two novel cysteine frameworks. While highly expressed transcripts were common to both specimens, variation of moderately and weakly expressed precursor sequences was surprisingly diverse, with one specimen expressing two unique gene superfamilies and consistently producing more paralogs within each conotoxin gene superfamily. Using a quantitative labelling method, conotoxin variability was compared quantitatively, with highly expressed peptides showing a strong correlation between transcription and translation, whereas peptides expressed at lower levels showed a poor correlation. These results suggest that major transcripts are subject to stabilizing selection, while minor transcripts are subject to diversifying selection. MDPI 2019-03-19 /pmc/articles/PMC6471084/ /pubmed/30893765 http://dx.doi.org/10.3390/md17030177 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jin, Ai-Hua
Dutertre, Sébastien
Dutt, Mriga
Lavergne, Vincent
Jones, Alun
Lewis, Richard J.
Alewood, Paul F.
Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis
title Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis
title_full Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis
title_fullStr Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis
title_full_unstemmed Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis
title_short Transcriptomic-Proteomic Correlation in the Predation-Evoked Venom of the Cone Snail, Conus imperialis
title_sort transcriptomic-proteomic correlation in the predation-evoked venom of the cone snail, conus imperialis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471084/
https://www.ncbi.nlm.nih.gov/pubmed/30893765
http://dx.doi.org/10.3390/md17030177
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