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PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults

The gut microbiota is increasingly recognized as an important modulator of human health. As such, there is a growing need to identify effective means of selectively modifying gut microbial communities. Bacteriophages, which were briefly utilized as clinical antimicrobials in the early 20th century,...

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Autores principales: Febvre, Hallie P., Rao, Sangeeta, Gindin, Melinda, Goodwin, Natalie D. M., Finer, Elijah, Vivanco, Jorge S., Lu, Shen, Manter, Daniel K., Wallace, Taylor C., Weir, Tiffany L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471193/
https://www.ncbi.nlm.nih.gov/pubmed/30897686
http://dx.doi.org/10.3390/nu11030666
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author Febvre, Hallie P.
Rao, Sangeeta
Gindin, Melinda
Goodwin, Natalie D. M.
Finer, Elijah
Vivanco, Jorge S.
Lu, Shen
Manter, Daniel K.
Wallace, Taylor C.
Weir, Tiffany L.
author_facet Febvre, Hallie P.
Rao, Sangeeta
Gindin, Melinda
Goodwin, Natalie D. M.
Finer, Elijah
Vivanco, Jorge S.
Lu, Shen
Manter, Daniel K.
Wallace, Taylor C.
Weir, Tiffany L.
author_sort Febvre, Hallie P.
collection PubMed
description The gut microbiota is increasingly recognized as an important modulator of human health. As such, there is a growing need to identify effective means of selectively modifying gut microbial communities. Bacteriophages, which were briefly utilized as clinical antimicrobials in the early 20th century, present an opportunity to selectively reduce populations of undesirable microorganisms. However, whether intentional consumption of specific bacteriophages affects overall gut ecology is not yet known. Using a commercial cocktail of Escherichia coli-targeting bacteriophages, we examined their effects on gut microbiota and markers of intestinal and systemic inflammation in a healthy human population. In a double-blinded, placebo-controlled crossover trial, normal to overweight adults consumed bacteriophages for 28 days. Stool and blood samples were collected and used to examine inflammatory markers, lipid metabolism, and gut microbiota. Reductions in fecal E. coli loads were observed with phage consumption. However, there were no significant changes to alpha and beta diversity parameters, suggesting that consumed phages did not globally disrupt the microbiota. However, specific populations were altered in response to treatment, including increases in members of the butyrate-producing genera Eubacterium and a decreased proportion of taxa most closely related to Clostridium perfringens. Short-chain fatty acid production, inflammatory markers, and lipid metabolism were largely unaltered, but there was a small but significant decrease in circulating interleukin-4 (Il-4). Together, these data demonstrate the potential of bacteriophages to selectively reduce target organisms without global disruption of the gut community.
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spelling pubmed-64711932019-04-25 PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults Febvre, Hallie P. Rao, Sangeeta Gindin, Melinda Goodwin, Natalie D. M. Finer, Elijah Vivanco, Jorge S. Lu, Shen Manter, Daniel K. Wallace, Taylor C. Weir, Tiffany L. Nutrients Article The gut microbiota is increasingly recognized as an important modulator of human health. As such, there is a growing need to identify effective means of selectively modifying gut microbial communities. Bacteriophages, which were briefly utilized as clinical antimicrobials in the early 20th century, present an opportunity to selectively reduce populations of undesirable microorganisms. However, whether intentional consumption of specific bacteriophages affects overall gut ecology is not yet known. Using a commercial cocktail of Escherichia coli-targeting bacteriophages, we examined their effects on gut microbiota and markers of intestinal and systemic inflammation in a healthy human population. In a double-blinded, placebo-controlled crossover trial, normal to overweight adults consumed bacteriophages for 28 days. Stool and blood samples were collected and used to examine inflammatory markers, lipid metabolism, and gut microbiota. Reductions in fecal E. coli loads were observed with phage consumption. However, there were no significant changes to alpha and beta diversity parameters, suggesting that consumed phages did not globally disrupt the microbiota. However, specific populations were altered in response to treatment, including increases in members of the butyrate-producing genera Eubacterium and a decreased proportion of taxa most closely related to Clostridium perfringens. Short-chain fatty acid production, inflammatory markers, and lipid metabolism were largely unaltered, but there was a small but significant decrease in circulating interleukin-4 (Il-4). Together, these data demonstrate the potential of bacteriophages to selectively reduce target organisms without global disruption of the gut community. MDPI 2019-03-20 /pmc/articles/PMC6471193/ /pubmed/30897686 http://dx.doi.org/10.3390/nu11030666 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Febvre, Hallie P.
Rao, Sangeeta
Gindin, Melinda
Goodwin, Natalie D. M.
Finer, Elijah
Vivanco, Jorge S.
Lu, Shen
Manter, Daniel K.
Wallace, Taylor C.
Weir, Tiffany L.
PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults
title PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults
title_full PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults
title_fullStr PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults
title_full_unstemmed PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults
title_short PHAGE Study: Effects of Supplemental Bacteriophage Intake on Inflammation and Gut Microbiota in Healthy Adults
title_sort phage study: effects of supplemental bacteriophage intake on inflammation and gut microbiota in healthy adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471193/
https://www.ncbi.nlm.nih.gov/pubmed/30897686
http://dx.doi.org/10.3390/nu11030666
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