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Metabolism of Stilbenoids by Human Faecal Microbiota

Stilbenoids are dietary phenolics with notable biological effects on humans. Epidemiological, clinical, and nutritional studies from recent years have confirmed the significant biological effects of stilbenoids, such as oxidative stress protection and the prevention of degenerative diseases, includi...

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Autores principales: Jarosova, Veronika, Vesely, Ondrej, Marsik, Petr, Jaimes, Jose Diogenes, Smejkal, Karel, Kloucek, Pavel, Havlik, Jaroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471231/
https://www.ncbi.nlm.nih.gov/pubmed/30909544
http://dx.doi.org/10.3390/molecules24061155
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author Jarosova, Veronika
Vesely, Ondrej
Marsik, Petr
Jaimes, Jose Diogenes
Smejkal, Karel
Kloucek, Pavel
Havlik, Jaroslav
author_facet Jarosova, Veronika
Vesely, Ondrej
Marsik, Petr
Jaimes, Jose Diogenes
Smejkal, Karel
Kloucek, Pavel
Havlik, Jaroslav
author_sort Jarosova, Veronika
collection PubMed
description Stilbenoids are dietary phenolics with notable biological effects on humans. Epidemiological, clinical, and nutritional studies from recent years have confirmed the significant biological effects of stilbenoids, such as oxidative stress protection and the prevention of degenerative diseases, including cancer, cardiovascular diseases, and neurodegenerative diseases. Stilbenoids are intensively metabolically transformed by colon microbiota, and their corresponding metabolites might show different or stronger biological activity than their parent molecules. The aim of the present study was to determine the metabolism of six stilbenoids (resveratrol, oxyresveratrol, piceatannol, thunalbene, batatasin III, and pinostilbene), mediated by colon microbiota. Stilbenoids were fermented in an in vitro faecal fermentation system using fresh faeces from five different donors as an inoculum. The samples of metabolized stilbenoids were collected at 0, 2, 4, 8, 24, and 48 h. Significant differences in the microbial transformation among stilbene derivatives were observed by liquid chromatography mass spectrometry (LC/MS). Four stilbenoids (resveratrol, oxyresveratrol, piceatannol and thunalbene) were metabolically transformed by double bond reduction, dihydroxylation, and demethylation, while batatasin III and pinostilbene were stable under conditions simulating the colon environment. Strong inter-individual differences in speed, intensity, and pathways of metabolism were observed among the faecal samples obtained from the donors.
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spelling pubmed-64712312019-04-26 Metabolism of Stilbenoids by Human Faecal Microbiota Jarosova, Veronika Vesely, Ondrej Marsik, Petr Jaimes, Jose Diogenes Smejkal, Karel Kloucek, Pavel Havlik, Jaroslav Molecules Article Stilbenoids are dietary phenolics with notable biological effects on humans. Epidemiological, clinical, and nutritional studies from recent years have confirmed the significant biological effects of stilbenoids, such as oxidative stress protection and the prevention of degenerative diseases, including cancer, cardiovascular diseases, and neurodegenerative diseases. Stilbenoids are intensively metabolically transformed by colon microbiota, and their corresponding metabolites might show different or stronger biological activity than their parent molecules. The aim of the present study was to determine the metabolism of six stilbenoids (resveratrol, oxyresveratrol, piceatannol, thunalbene, batatasin III, and pinostilbene), mediated by colon microbiota. Stilbenoids were fermented in an in vitro faecal fermentation system using fresh faeces from five different donors as an inoculum. The samples of metabolized stilbenoids were collected at 0, 2, 4, 8, 24, and 48 h. Significant differences in the microbial transformation among stilbene derivatives were observed by liquid chromatography mass spectrometry (LC/MS). Four stilbenoids (resveratrol, oxyresveratrol, piceatannol and thunalbene) were metabolically transformed by double bond reduction, dihydroxylation, and demethylation, while batatasin III and pinostilbene were stable under conditions simulating the colon environment. Strong inter-individual differences in speed, intensity, and pathways of metabolism were observed among the faecal samples obtained from the donors. MDPI 2019-03-23 /pmc/articles/PMC6471231/ /pubmed/30909544 http://dx.doi.org/10.3390/molecules24061155 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jarosova, Veronika
Vesely, Ondrej
Marsik, Petr
Jaimes, Jose Diogenes
Smejkal, Karel
Kloucek, Pavel
Havlik, Jaroslav
Metabolism of Stilbenoids by Human Faecal Microbiota
title Metabolism of Stilbenoids by Human Faecal Microbiota
title_full Metabolism of Stilbenoids by Human Faecal Microbiota
title_fullStr Metabolism of Stilbenoids by Human Faecal Microbiota
title_full_unstemmed Metabolism of Stilbenoids by Human Faecal Microbiota
title_short Metabolism of Stilbenoids by Human Faecal Microbiota
title_sort metabolism of stilbenoids by human faecal microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471231/
https://www.ncbi.nlm.nih.gov/pubmed/30909544
http://dx.doi.org/10.3390/molecules24061155
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