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An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction

The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processin...

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Autores principales: Tong, Rui, Zhang, Lijuan, Hu, Chuandeng, Chen, Xuee, Song, Qi, Lou, Kai, Tang, Xin, Chen, Yongsheng, Gong, Xiuqing, Gao, Yibo, Wen, Weijia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471407/
https://www.ncbi.nlm.nih.gov/pubmed/30909465
http://dx.doi.org/10.3390/mi10030204
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author Tong, Rui
Zhang, Lijuan
Hu, Chuandeng
Chen, Xuee
Song, Qi
Lou, Kai
Tang, Xin
Chen, Yongsheng
Gong, Xiuqing
Gao, Yibo
Wen, Weijia
author_facet Tong, Rui
Zhang, Lijuan
Hu, Chuandeng
Chen, Xuee
Song, Qi
Lou, Kai
Tang, Xin
Chen, Yongsheng
Gong, Xiuqing
Gao, Yibo
Wen, Weijia
author_sort Tong, Rui
collection PubMed
description The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processing disc and its associated rotary power output mechanism, a pipetting module, a magnet module and an external central controller to enable a customizable and automated robust nucleic acid sample preparation. The extracted nucleic acid using 293T cells were verified using real-time polymerase chain reaction (PCR) and the data implies a comparable efficiency to a manual process, with the advantages of performing a flexible, time-saving (~10 min), and simple nucleic acid sample preparation.
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spelling pubmed-64714072019-04-27 An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction Tong, Rui Zhang, Lijuan Hu, Chuandeng Chen, Xuee Song, Qi Lou, Kai Tang, Xin Chen, Yongsheng Gong, Xiuqing Gao, Yibo Wen, Weijia Micromachines (Basel) Article The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processing disc and its associated rotary power output mechanism, a pipetting module, a magnet module and an external central controller to enable a customizable and automated robust nucleic acid sample preparation. The extracted nucleic acid using 293T cells were verified using real-time polymerase chain reaction (PCR) and the data implies a comparable efficiency to a manual process, with the advantages of performing a flexible, time-saving (~10 min), and simple nucleic acid sample preparation. MDPI 2019-03-22 /pmc/articles/PMC6471407/ /pubmed/30909465 http://dx.doi.org/10.3390/mi10030204 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tong, Rui
Zhang, Lijuan
Hu, Chuandeng
Chen, Xuee
Song, Qi
Lou, Kai
Tang, Xin
Chen, Yongsheng
Gong, Xiuqing
Gao, Yibo
Wen, Weijia
An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
title An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
title_full An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
title_fullStr An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
title_full_unstemmed An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
title_short An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
title_sort automated and miniaturized rotating-disk device for rapid nucleic acid extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471407/
https://www.ncbi.nlm.nih.gov/pubmed/30909465
http://dx.doi.org/10.3390/mi10030204
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