Cargando…
An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processin...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471407/ https://www.ncbi.nlm.nih.gov/pubmed/30909465 http://dx.doi.org/10.3390/mi10030204 |
_version_ | 1783412021941764096 |
---|---|
author | Tong, Rui Zhang, Lijuan Hu, Chuandeng Chen, Xuee Song, Qi Lou, Kai Tang, Xin Chen, Yongsheng Gong, Xiuqing Gao, Yibo Wen, Weijia |
author_facet | Tong, Rui Zhang, Lijuan Hu, Chuandeng Chen, Xuee Song, Qi Lou, Kai Tang, Xin Chen, Yongsheng Gong, Xiuqing Gao, Yibo Wen, Weijia |
author_sort | Tong, Rui |
collection | PubMed |
description | The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processing disc and its associated rotary power output mechanism, a pipetting module, a magnet module and an external central controller to enable a customizable and automated robust nucleic acid sample preparation. The extracted nucleic acid using 293T cells were verified using real-time polymerase chain reaction (PCR) and the data implies a comparable efficiency to a manual process, with the advantages of performing a flexible, time-saving (~10 min), and simple nucleic acid sample preparation. |
format | Online Article Text |
id | pubmed-6471407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64714072019-04-27 An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction Tong, Rui Zhang, Lijuan Hu, Chuandeng Chen, Xuee Song, Qi Lou, Kai Tang, Xin Chen, Yongsheng Gong, Xiuqing Gao, Yibo Wen, Weijia Micromachines (Basel) Article The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processing disc and its associated rotary power output mechanism, a pipetting module, a magnet module and an external central controller to enable a customizable and automated robust nucleic acid sample preparation. The extracted nucleic acid using 293T cells were verified using real-time polymerase chain reaction (PCR) and the data implies a comparable efficiency to a manual process, with the advantages of performing a flexible, time-saving (~10 min), and simple nucleic acid sample preparation. MDPI 2019-03-22 /pmc/articles/PMC6471407/ /pubmed/30909465 http://dx.doi.org/10.3390/mi10030204 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tong, Rui Zhang, Lijuan Hu, Chuandeng Chen, Xuee Song, Qi Lou, Kai Tang, Xin Chen, Yongsheng Gong, Xiuqing Gao, Yibo Wen, Weijia An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction |
title | An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction |
title_full | An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction |
title_fullStr | An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction |
title_full_unstemmed | An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction |
title_short | An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction |
title_sort | automated and miniaturized rotating-disk device for rapid nucleic acid extraction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471407/ https://www.ncbi.nlm.nih.gov/pubmed/30909465 http://dx.doi.org/10.3390/mi10030204 |
work_keys_str_mv | AT tongrui anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT zhanglijuan anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT huchuandeng anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT chenxuee anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT songqi anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT loukai anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT tangxin anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT chenyongsheng anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT gongxiuqing anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT gaoyibo anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT wenweijia anautomatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT tongrui automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT zhanglijuan automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT huchuandeng automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT chenxuee automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT songqi automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT loukai automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT tangxin automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT chenyongsheng automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT gongxiuqing automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT gaoyibo automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction AT wenweijia automatedandminiaturizedrotatingdiskdeviceforrapidnucleicacidextraction |