Cargando…

Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese

Under physiological conditions, the small intestine represents a barrier against harmful antigens and pathogens. Maintaining of the intestinal barrier depends largely on cell–cell interactions (adherent-junctions) and cell–matrix interactions (tight-junctions). Inflammatory bowel disease is characte...

Descripción completa

Detalles Bibliográficos
Autores principales: Tenore, Gian Carlo, Pagano, Ester, Lama, Stefania, Vanacore, Daniela, Di Maro, Salvatore, Maisto, Maria, Capasso, Raffaele, Merlino, Francesco, Borrelli, Francesca, Stiuso, Paola, Novellino, Ettore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471453/
https://www.ncbi.nlm.nih.gov/pubmed/30871183
http://dx.doi.org/10.3390/nu11030610
_version_ 1783412032747339776
author Tenore, Gian Carlo
Pagano, Ester
Lama, Stefania
Vanacore, Daniela
Di Maro, Salvatore
Maisto, Maria
Capasso, Raffaele
Merlino, Francesco
Borrelli, Francesca
Stiuso, Paola
Novellino, Ettore
author_facet Tenore, Gian Carlo
Pagano, Ester
Lama, Stefania
Vanacore, Daniela
Di Maro, Salvatore
Maisto, Maria
Capasso, Raffaele
Merlino, Francesco
Borrelli, Francesca
Stiuso, Paola
Novellino, Ettore
author_sort Tenore, Gian Carlo
collection PubMed
description Under physiological conditions, the small intestine represents a barrier against harmful antigens and pathogens. Maintaining of the intestinal barrier depends largely on cell–cell interactions (adherent-junctions) and cell–matrix interactions (tight-junctions). Inflammatory bowel disease is characterized by chronic inflammation, which induces a destructuring of the architecture junctional epithelial proteins with consequent rupture of the intestinal barrier. Recently, a peptide identified by Bubalus bubalis milk-derived products (MBCP) has been able to reduce oxidative stress in intestinal epithelial cells and erythrocytes. Our aim was to evaluate the therapeutic potential of MBCP in inflammatory bowel disease (IBD). We studied the effect of MBCP on (i) inflamed human intestinal Caco2 cells and (ii) dinitrobenzene sulfonic acid (DNBS) mice model of colitis. We have shown that MBCP, at non-cytotoxic concentrations, both in vitro and in vivo induced the adherent epithelial junctions organization, modulated the nuclear factor (NF)-κB pathway and reduced the intestinal permeability. Furthermore, the MBCP reverted the atropine and tubocurarine injury effects on adherent-junctions. The data obtained showed that MBCP possesses anti-inflammatory effects both in vitro and in vivo. These results could have an important impact on the therapeutic potential of MBCP in helping to restore the intestinal epithelium integrity damaged by inflammation.
format Online
Article
Text
id pubmed-6471453
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64714532019-04-25 Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese Tenore, Gian Carlo Pagano, Ester Lama, Stefania Vanacore, Daniela Di Maro, Salvatore Maisto, Maria Capasso, Raffaele Merlino, Francesco Borrelli, Francesca Stiuso, Paola Novellino, Ettore Nutrients Article Under physiological conditions, the small intestine represents a barrier against harmful antigens and pathogens. Maintaining of the intestinal barrier depends largely on cell–cell interactions (adherent-junctions) and cell–matrix interactions (tight-junctions). Inflammatory bowel disease is characterized by chronic inflammation, which induces a destructuring of the architecture junctional epithelial proteins with consequent rupture of the intestinal barrier. Recently, a peptide identified by Bubalus bubalis milk-derived products (MBCP) has been able to reduce oxidative stress in intestinal epithelial cells and erythrocytes. Our aim was to evaluate the therapeutic potential of MBCP in inflammatory bowel disease (IBD). We studied the effect of MBCP on (i) inflamed human intestinal Caco2 cells and (ii) dinitrobenzene sulfonic acid (DNBS) mice model of colitis. We have shown that MBCP, at non-cytotoxic concentrations, both in vitro and in vivo induced the adherent epithelial junctions organization, modulated the nuclear factor (NF)-κB pathway and reduced the intestinal permeability. Furthermore, the MBCP reverted the atropine and tubocurarine injury effects on adherent-junctions. The data obtained showed that MBCP possesses anti-inflammatory effects both in vitro and in vivo. These results could have an important impact on the therapeutic potential of MBCP in helping to restore the intestinal epithelium integrity damaged by inflammation. MDPI 2019-03-13 /pmc/articles/PMC6471453/ /pubmed/30871183 http://dx.doi.org/10.3390/nu11030610 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tenore, Gian Carlo
Pagano, Ester
Lama, Stefania
Vanacore, Daniela
Di Maro, Salvatore
Maisto, Maria
Capasso, Raffaele
Merlino, Francesco
Borrelli, Francesca
Stiuso, Paola
Novellino, Ettore
Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese
title Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese
title_full Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese
title_fullStr Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese
title_full_unstemmed Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese
title_short Intestinal Anti-Inflammatory Effect of a Peptide Derived from Gastrointestinal Digestion of Buffalo (Bubalus bubalis) Mozzarella Cheese
title_sort intestinal anti-inflammatory effect of a peptide derived from gastrointestinal digestion of buffalo (bubalus bubalis) mozzarella cheese
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471453/
https://www.ncbi.nlm.nih.gov/pubmed/30871183
http://dx.doi.org/10.3390/nu11030610
work_keys_str_mv AT tenoregiancarlo intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT paganoester intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT lamastefania intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT vanacoredaniela intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT dimarosalvatore intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT maistomaria intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT capassoraffaele intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT merlinofrancesco intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT borrellifrancesca intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT stiusopaola intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese
AT novellinoettore intestinalantiinflammatoryeffectofapeptidederivedfromgastrointestinaldigestionofbuffalobubalusbubalismozzarellacheese