Cargando…
Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering
Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471706/ https://www.ncbi.nlm.nih.gov/pubmed/30889841 http://dx.doi.org/10.3390/ijms20061357 |
_version_ | 1783412085635416064 |
---|---|
author | Mann, Vivek Grimm, Daniela Corydon, Thomas J Krüger, Marcus Wehland, Markus Riwaldt, Stefan Sahana, Jayashree Kopp, Sascha Bauer, Johann Reseland, Janne E. Infanger, Manfred Mari Lian, Aina Okoro, Elvis Sundaresan, Alamelu |
author_facet | Mann, Vivek Grimm, Daniela Corydon, Thomas J Krüger, Marcus Wehland, Markus Riwaldt, Stefan Sahana, Jayashree Kopp, Sascha Bauer, Johann Reseland, Janne E. Infanger, Manfred Mari Lian, Aina Okoro, Elvis Sundaresan, Alamelu |
author_sort | Mann, Vivek |
collection | PubMed |
description | Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries. |
format | Online Article Text |
id | pubmed-6471706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64717062019-04-26 Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering Mann, Vivek Grimm, Daniela Corydon, Thomas J Krüger, Marcus Wehland, Markus Riwaldt, Stefan Sahana, Jayashree Kopp, Sascha Bauer, Johann Reseland, Janne E. Infanger, Manfred Mari Lian, Aina Okoro, Elvis Sundaresan, Alamelu Int J Mol Sci Article Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries. MDPI 2019-03-18 /pmc/articles/PMC6471706/ /pubmed/30889841 http://dx.doi.org/10.3390/ijms20061357 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mann, Vivek Grimm, Daniela Corydon, Thomas J Krüger, Marcus Wehland, Markus Riwaldt, Stefan Sahana, Jayashree Kopp, Sascha Bauer, Johann Reseland, Janne E. Infanger, Manfred Mari Lian, Aina Okoro, Elvis Sundaresan, Alamelu Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering |
title | Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering |
title_full | Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering |
title_fullStr | Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering |
title_full_unstemmed | Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering |
title_short | Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering |
title_sort | changes in human foetal osteoblasts exposed to the random positioning machine and bone construct tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471706/ https://www.ncbi.nlm.nih.gov/pubmed/30889841 http://dx.doi.org/10.3390/ijms20061357 |
work_keys_str_mv | AT mannvivek changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT grimmdaniela changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT corydonthomasj changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT krugermarcus changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT wehlandmarkus changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT riwaldtstefan changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT sahanajayashree changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT koppsascha changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT bauerjohann changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT reselandjannee changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT infangermanfred changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT marilianaina changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT okoroelvis changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering AT sundaresanalamelu changesinhumanfoetalosteoblastsexposedtotherandompositioningmachineandboneconstructtissueengineering |