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Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering

Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (...

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Autores principales: Mann, Vivek, Grimm, Daniela, Corydon, Thomas J, Krüger, Marcus, Wehland, Markus, Riwaldt, Stefan, Sahana, Jayashree, Kopp, Sascha, Bauer, Johann, Reseland, Janne E., Infanger, Manfred, Mari Lian, Aina, Okoro, Elvis, Sundaresan, Alamelu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471706/
https://www.ncbi.nlm.nih.gov/pubmed/30889841
http://dx.doi.org/10.3390/ijms20061357
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author Mann, Vivek
Grimm, Daniela
Corydon, Thomas J
Krüger, Marcus
Wehland, Markus
Riwaldt, Stefan
Sahana, Jayashree
Kopp, Sascha
Bauer, Johann
Reseland, Janne E.
Infanger, Manfred
Mari Lian, Aina
Okoro, Elvis
Sundaresan, Alamelu
author_facet Mann, Vivek
Grimm, Daniela
Corydon, Thomas J
Krüger, Marcus
Wehland, Markus
Riwaldt, Stefan
Sahana, Jayashree
Kopp, Sascha
Bauer, Johann
Reseland, Janne E.
Infanger, Manfred
Mari Lian, Aina
Okoro, Elvis
Sundaresan, Alamelu
author_sort Mann, Vivek
collection PubMed
description Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries.
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spelling pubmed-64717062019-04-26 Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering Mann, Vivek Grimm, Daniela Corydon, Thomas J Krüger, Marcus Wehland, Markus Riwaldt, Stefan Sahana, Jayashree Kopp, Sascha Bauer, Johann Reseland, Janne E. Infanger, Manfred Mari Lian, Aina Okoro, Elvis Sundaresan, Alamelu Int J Mol Sci Article Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries. MDPI 2019-03-18 /pmc/articles/PMC6471706/ /pubmed/30889841 http://dx.doi.org/10.3390/ijms20061357 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mann, Vivek
Grimm, Daniela
Corydon, Thomas J
Krüger, Marcus
Wehland, Markus
Riwaldt, Stefan
Sahana, Jayashree
Kopp, Sascha
Bauer, Johann
Reseland, Janne E.
Infanger, Manfred
Mari Lian, Aina
Okoro, Elvis
Sundaresan, Alamelu
Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering
title Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering
title_full Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering
title_fullStr Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering
title_full_unstemmed Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering
title_short Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering
title_sort changes in human foetal osteoblasts exposed to the random positioning machine and bone construct tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471706/
https://www.ncbi.nlm.nih.gov/pubmed/30889841
http://dx.doi.org/10.3390/ijms20061357
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