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H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells

Background: Porcine skeletal muscle satellite cells play important roles in myogenesis and muscle regeneration. Integrated analysis of transcriptome and histone modifications would reveal epigenomic roles in promoting myogenic differentiation in swine. Methods: Porcine satellite cells (PSCs) were is...

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Autores principales: Wang, Sheng, Sun, Yan, Ren, Ruimin, Xie, Junhui, Tian, Xiaohuan, Zhao, Shuhong, Li, Xinyun, Cao, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471710/
https://www.ncbi.nlm.nih.gov/pubmed/30893875
http://dx.doi.org/10.3390/genes10030231
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author Wang, Sheng
Sun, Yan
Ren, Ruimin
Xie, Junhui
Tian, Xiaohuan
Zhao, Shuhong
Li, Xinyun
Cao, Jianhua
author_facet Wang, Sheng
Sun, Yan
Ren, Ruimin
Xie, Junhui
Tian, Xiaohuan
Zhao, Shuhong
Li, Xinyun
Cao, Jianhua
author_sort Wang, Sheng
collection PubMed
description Background: Porcine skeletal muscle satellite cells play important roles in myogenesis and muscle regeneration. Integrated analysis of transcriptome and histone modifications would reveal epigenomic roles in promoting myogenic differentiation in swine. Methods: Porcine satellite cells (PSCs) were isolated and in-vitro cultured from newborn piglets. RNA Sequencing (RNA-Seq) and Chromatin Immunoprecipitation Sequencing (ChIP-Seq) experiments were performed using proliferating cells and terminal myotubes in order to interrogate the transcriptomic profiles, as well as the distribution of histone markers—H3K4me3, H3K27me3, and H3K27ac—and RNA polymerase II. Results: The study identified 917 differentially expressed genes during cell differentiation. The landscape of epigenetic marks was displayed on a genome-wide scale, which had globally shrunken. H3K27me3 reinforcement participated in obstructing the transcription of proliferation-related genes, while its depletion was closely related to the up-regulation of myogenic genes. Furthermore, the degree of H3K27me3 modification was dramatically reduced by 50%, and 139 myogenic genes were upregulated to promote cell differentiation. Conclusions: The depletion of H3K27me3 was shown to promote porcine satellite cell differentiation through upregulating the transcription level of myogenic genes. Our findings in this study provide new insights of the epigenomic mechanisms occurring during myogenic differentiation, and shed light on chromatin states and the dynamics underlying myogenesis.
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spelling pubmed-64717102019-04-27 H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells Wang, Sheng Sun, Yan Ren, Ruimin Xie, Junhui Tian, Xiaohuan Zhao, Shuhong Li, Xinyun Cao, Jianhua Genes (Basel) Article Background: Porcine skeletal muscle satellite cells play important roles in myogenesis and muscle regeneration. Integrated analysis of transcriptome and histone modifications would reveal epigenomic roles in promoting myogenic differentiation in swine. Methods: Porcine satellite cells (PSCs) were isolated and in-vitro cultured from newborn piglets. RNA Sequencing (RNA-Seq) and Chromatin Immunoprecipitation Sequencing (ChIP-Seq) experiments were performed using proliferating cells and terminal myotubes in order to interrogate the transcriptomic profiles, as well as the distribution of histone markers—H3K4me3, H3K27me3, and H3K27ac—and RNA polymerase II. Results: The study identified 917 differentially expressed genes during cell differentiation. The landscape of epigenetic marks was displayed on a genome-wide scale, which had globally shrunken. H3K27me3 reinforcement participated in obstructing the transcription of proliferation-related genes, while its depletion was closely related to the up-regulation of myogenic genes. Furthermore, the degree of H3K27me3 modification was dramatically reduced by 50%, and 139 myogenic genes were upregulated to promote cell differentiation. Conclusions: The depletion of H3K27me3 was shown to promote porcine satellite cell differentiation through upregulating the transcription level of myogenic genes. Our findings in this study provide new insights of the epigenomic mechanisms occurring during myogenic differentiation, and shed light on chromatin states and the dynamics underlying myogenesis. MDPI 2019-03-19 /pmc/articles/PMC6471710/ /pubmed/30893875 http://dx.doi.org/10.3390/genes10030231 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Sheng
Sun, Yan
Ren, Ruimin
Xie, Junhui
Tian, Xiaohuan
Zhao, Shuhong
Li, Xinyun
Cao, Jianhua
H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells
title H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells
title_full H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells
title_fullStr H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells
title_full_unstemmed H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells
title_short H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells
title_sort h3k27me3 depletion during differentiation promotes myogenic transcription in porcine satellite cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471710/
https://www.ncbi.nlm.nih.gov/pubmed/30893875
http://dx.doi.org/10.3390/genes10030231
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