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Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS

Research on microcirculatory alterations in human heart disease is essential to understand the genesis of myocardial contractile dysfunction and its evolution towards heart failure. The use of contrast agents in magnetic resonance imaging is an important tool in medical diagnostics related to this d...

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Autores principales: Bonechi, Claudia, Consumi, Marco, Matteucci, Marco, Tamasi, Gabriella, Donati, Alessandro, Leone, Gemma, Menichetti, Luca, Kusmic, Claudia, Rossi, Claudio, Magnani, Agnese
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471734/
https://www.ncbi.nlm.nih.gov/pubmed/30884846
http://dx.doi.org/10.3390/ijms20061339
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author Bonechi, Claudia
Consumi, Marco
Matteucci, Marco
Tamasi, Gabriella
Donati, Alessandro
Leone, Gemma
Menichetti, Luca
Kusmic, Claudia
Rossi, Claudio
Magnani, Agnese
author_facet Bonechi, Claudia
Consumi, Marco
Matteucci, Marco
Tamasi, Gabriella
Donati, Alessandro
Leone, Gemma
Menichetti, Luca
Kusmic, Claudia
Rossi, Claudio
Magnani, Agnese
author_sort Bonechi, Claudia
collection PubMed
description Research on microcirculatory alterations in human heart disease is essential to understand the genesis of myocardial contractile dysfunction and its evolution towards heart failure. The use of contrast agents in magnetic resonance imaging is an important tool in medical diagnostics related to this dysfunction. Contrast agents significantly improve the imaging by enhancing the nuclear magnetic relaxation rates of water protons in the tissues where they are distributed. Gadolinium complexes are widely employed in clinical practice due to their high magnetic moment and relatively long electronic relaxation time. In this study, the behavior of gadolinium ion as a contrast agent was investigated by two complementary methods, relaxometry and secondary ion mass spectrometry. The study examined the distribution of blood flow within the microvascular network in ex vivo Langendorff isolated rat heart models, perfused with Omniscan(®) contrast agent. The combined use of secondary ion mass spectrometry and relaxometry allowed for both a qualitative mapping of agent distribution as well as the quantification of gadolinium ion concentration and persistence. This combination of a chemical mapping and temporal analysis of the molar concentration of gadolinium ion in heart tissue allows for new insights on the biomolecular mechanisms underlying the microcirculatory alterations in heart disease.
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spelling pubmed-64717342019-04-26 Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS Bonechi, Claudia Consumi, Marco Matteucci, Marco Tamasi, Gabriella Donati, Alessandro Leone, Gemma Menichetti, Luca Kusmic, Claudia Rossi, Claudio Magnani, Agnese Int J Mol Sci Article Research on microcirculatory alterations in human heart disease is essential to understand the genesis of myocardial contractile dysfunction and its evolution towards heart failure. The use of contrast agents in magnetic resonance imaging is an important tool in medical diagnostics related to this dysfunction. Contrast agents significantly improve the imaging by enhancing the nuclear magnetic relaxation rates of water protons in the tissues where they are distributed. Gadolinium complexes are widely employed in clinical practice due to their high magnetic moment and relatively long electronic relaxation time. In this study, the behavior of gadolinium ion as a contrast agent was investigated by two complementary methods, relaxometry and secondary ion mass spectrometry. The study examined the distribution of blood flow within the microvascular network in ex vivo Langendorff isolated rat heart models, perfused with Omniscan(®) contrast agent. The combined use of secondary ion mass spectrometry and relaxometry allowed for both a qualitative mapping of agent distribution as well as the quantification of gadolinium ion concentration and persistence. This combination of a chemical mapping and temporal analysis of the molar concentration of gadolinium ion in heart tissue allows for new insights on the biomolecular mechanisms underlying the microcirculatory alterations in heart disease. MDPI 2019-03-16 /pmc/articles/PMC6471734/ /pubmed/30884846 http://dx.doi.org/10.3390/ijms20061339 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bonechi, Claudia
Consumi, Marco
Matteucci, Marco
Tamasi, Gabriella
Donati, Alessandro
Leone, Gemma
Menichetti, Luca
Kusmic, Claudia
Rossi, Claudio
Magnani, Agnese
Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS
title Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS
title_full Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS
title_fullStr Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS
title_full_unstemmed Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS
title_short Distribution of Gadolinium in Rat Heart Studied by Fast Field Cycling Relaxometry and Imaging SIMS
title_sort distribution of gadolinium in rat heart studied by fast field cycling relaxometry and imaging sims
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471734/
https://www.ncbi.nlm.nih.gov/pubmed/30884846
http://dx.doi.org/10.3390/ijms20061339
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