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Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats

Increasing evidence has suggested that hepatic lipid accumulation is associated with hepatic insulin resistance; however, the underlying mechanism is yet to be determined. It was demonstrated that the levels of microRNA-215 (miR-215) expression in the liver of rats fed a high-fat diet were significa...

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Autores principales: Li, Wei-Dong, Xia, Jin-Rong, Lian, Yan-Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471735/
https://www.ncbi.nlm.nih.gov/pubmed/30896868
http://dx.doi.org/10.3892/mmr.2019.10031
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author Li, Wei-Dong
Xia, Jin-Rong
Lian, Yan-Shu
author_facet Li, Wei-Dong
Xia, Jin-Rong
Lian, Yan-Shu
author_sort Li, Wei-Dong
collection PubMed
description Increasing evidence has suggested that hepatic lipid accumulation is associated with hepatic insulin resistance; however, the underlying mechanism is yet to be determined. It was demonstrated that the levels of microRNA-215 (miR-215) expression in the liver of rats fed a high-fat diet were significantly increased compared with rats on a control diet. Additionally, it was revealed via luciferase assays and western blotting that miR-215 targets rapamycin-insensitive companion of mammalian target of rapamycin (Rictor), an important protein in the hepatic insulin signalling pathway. Following overexpression of miR-215 in the H4IIE rat hepatocarcinoma cell line, it was reported that the intracellular insulin signalling pathway was inhibited; conversely, inhibition of miR-215 expression induced this pathway. Furthermore, it was demonstrated via reverse transcription-quantitative polymerase chain reaction analysis that free fatty acids promoted the expression of miR-215. The present study provided a novel mechanistic insight into the association between nonalcoholic fatty liver and hepatic insulin resistance.
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spelling pubmed-64717352019-04-23 Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats Li, Wei-Dong Xia, Jin-Rong Lian, Yan-Shu Mol Med Rep Articles Increasing evidence has suggested that hepatic lipid accumulation is associated with hepatic insulin resistance; however, the underlying mechanism is yet to be determined. It was demonstrated that the levels of microRNA-215 (miR-215) expression in the liver of rats fed a high-fat diet were significantly increased compared with rats on a control diet. Additionally, it was revealed via luciferase assays and western blotting that miR-215 targets rapamycin-insensitive companion of mammalian target of rapamycin (Rictor), an important protein in the hepatic insulin signalling pathway. Following overexpression of miR-215 in the H4IIE rat hepatocarcinoma cell line, it was reported that the intracellular insulin signalling pathway was inhibited; conversely, inhibition of miR-215 expression induced this pathway. Furthermore, it was demonstrated via reverse transcription-quantitative polymerase chain reaction analysis that free fatty acids promoted the expression of miR-215. The present study provided a novel mechanistic insight into the association between nonalcoholic fatty liver and hepatic insulin resistance. D.A. Spandidos 2019-05 2019-03-14 /pmc/articles/PMC6471735/ /pubmed/30896868 http://dx.doi.org/10.3892/mmr.2019.10031 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Wei-Dong
Xia, Jin-Rong
Lian, Yan-Shu
Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats
title Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats
title_full Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats
title_fullStr Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats
title_full_unstemmed Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats
title_short Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats
title_sort hepatic mir-215 target rictor and modulation of hepatic insulin signalling in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471735/
https://www.ncbi.nlm.nih.gov/pubmed/30896868
http://dx.doi.org/10.3892/mmr.2019.10031
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