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Characterization of different biodegradable scaffolds in tissue engineering

The aim of the present study was to compare the characteristics of acellular dermal matrix (ADM), small intestinal submucosa (SIS) and Bio-Gide scaffolds with acellular vascular matrix (ACVM)-0.25% human-like collagen I (HLC-I) scaffold in tissue engineering blood vessels. The ACVM-0.25% HLC-I scaff...

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Autores principales: Qiu, Yan-Ling, Chen, Xiao, Hou, Ya-Li, Hou, Yan-Juan, Tian, Song-Bo, Chen, Yu-He, Yu, Li, Nie, Min-Hai, Liu, Xu-Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471812/
https://www.ncbi.nlm.nih.gov/pubmed/30896809
http://dx.doi.org/10.3892/mmr.2019.10066
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author Qiu, Yan-Ling
Chen, Xiao
Hou, Ya-Li
Hou, Yan-Juan
Tian, Song-Bo
Chen, Yu-He
Yu, Li
Nie, Min-Hai
Liu, Xu-Qian
author_facet Qiu, Yan-Ling
Chen, Xiao
Hou, Ya-Li
Hou, Yan-Juan
Tian, Song-Bo
Chen, Yu-He
Yu, Li
Nie, Min-Hai
Liu, Xu-Qian
author_sort Qiu, Yan-Ling
collection PubMed
description The aim of the present study was to compare the characteristics of acellular dermal matrix (ADM), small intestinal submucosa (SIS) and Bio-Gide scaffolds with acellular vascular matrix (ACVM)-0.25% human-like collagen I (HLC-I) scaffold in tissue engineering blood vessels. The ACVM-0.25% HLC-I scaffold was prepared and compared with ADM, SIS and Bio-Gide scaffolds via hematoxylin and eosin (H&E) staining, Masson staining and scanning electron microscope (SEM) observations. Primary human gingival fibroblasts (HGFs) were cultured and identified. Then, the experiment was established via the seeding of HGFs on different scaffolds for 1, 4 and 7 days. The compatibility of four different scaffolds with HGFs was evaluated by H&E staining, SEM observation and Cell Counting Kit-8 assay. Then, a series of experiments were conducted to evaluate water absorption capacities, mechanical abilities, the ultra-microstructure and the cytotoxicity of the four scaffolds. The ACVM-0.25% HLC-I scaffold was revealed to exhibit the best cell proliferation and good cell architecture. ADM and Bio-Gide scaffolds exhibited good mechanical stability but cell proliferation was reduced when compared with the ACVM-0.25% HLC-I scaffold. In addition, SIS scaffolds exhibited the worst cell proliferation. The ACVM-0.25% HLC-I scaffold exhibited the best water absorption, followed by the SIS and Bio-Gide scaffolds, and then the ADM scaffold. In conclusion, the ACVM-0.25% HLC-I scaffold has good mechanical properties as a tissue engineering scaffold and the present results suggest that it has better biological characterization when compared with other scaffold types.
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spelling pubmed-64718122019-04-23 Characterization of different biodegradable scaffolds in tissue engineering Qiu, Yan-Ling Chen, Xiao Hou, Ya-Li Hou, Yan-Juan Tian, Song-Bo Chen, Yu-He Yu, Li Nie, Min-Hai Liu, Xu-Qian Mol Med Rep Articles The aim of the present study was to compare the characteristics of acellular dermal matrix (ADM), small intestinal submucosa (SIS) and Bio-Gide scaffolds with acellular vascular matrix (ACVM)-0.25% human-like collagen I (HLC-I) scaffold in tissue engineering blood vessels. The ACVM-0.25% HLC-I scaffold was prepared and compared with ADM, SIS and Bio-Gide scaffolds via hematoxylin and eosin (H&E) staining, Masson staining and scanning electron microscope (SEM) observations. Primary human gingival fibroblasts (HGFs) were cultured and identified. Then, the experiment was established via the seeding of HGFs on different scaffolds for 1, 4 and 7 days. The compatibility of four different scaffolds with HGFs was evaluated by H&E staining, SEM observation and Cell Counting Kit-8 assay. Then, a series of experiments were conducted to evaluate water absorption capacities, mechanical abilities, the ultra-microstructure and the cytotoxicity of the four scaffolds. The ACVM-0.25% HLC-I scaffold was revealed to exhibit the best cell proliferation and good cell architecture. ADM and Bio-Gide scaffolds exhibited good mechanical stability but cell proliferation was reduced when compared with the ACVM-0.25% HLC-I scaffold. In addition, SIS scaffolds exhibited the worst cell proliferation. The ACVM-0.25% HLC-I scaffold exhibited the best water absorption, followed by the SIS and Bio-Gide scaffolds, and then the ADM scaffold. In conclusion, the ACVM-0.25% HLC-I scaffold has good mechanical properties as a tissue engineering scaffold and the present results suggest that it has better biological characterization when compared with other scaffold types. D.A. Spandidos 2019-05 2019-03-21 /pmc/articles/PMC6471812/ /pubmed/30896809 http://dx.doi.org/10.3892/mmr.2019.10066 Text en Copyright: © Qiu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Qiu, Yan-Ling
Chen, Xiao
Hou, Ya-Li
Hou, Yan-Juan
Tian, Song-Bo
Chen, Yu-He
Yu, Li
Nie, Min-Hai
Liu, Xu-Qian
Characterization of different biodegradable scaffolds in tissue engineering
title Characterization of different biodegradable scaffolds in tissue engineering
title_full Characterization of different biodegradable scaffolds in tissue engineering
title_fullStr Characterization of different biodegradable scaffolds in tissue engineering
title_full_unstemmed Characterization of different biodegradable scaffolds in tissue engineering
title_short Characterization of different biodegradable scaffolds in tissue engineering
title_sort characterization of different biodegradable scaffolds in tissue engineering
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471812/
https://www.ncbi.nlm.nih.gov/pubmed/30896809
http://dx.doi.org/10.3892/mmr.2019.10066
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