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Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study

Moyamoya disease (MMD) is a rare chronic cerebrovascular disease mainly found in individuals of East Asian ethnicity, and its pathogenesis is largely unknown. Transfer RNA-derived fragments (tRFs) are novel biological entities involved in many biological processes; however, whether tRFs contribute t...

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Autores principales: Wang, Chengjun, Zhao, Meng, Wang, Jia, Zhang, Dong, Wang, Shuo, Zhao, Jizong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472122/
https://www.ncbi.nlm.nih.gov/pubmed/30896793
http://dx.doi.org/10.3892/mmr.2019.10024
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author Wang, Chengjun
Zhao, Meng
Wang, Jia
Zhang, Dong
Wang, Shuo
Zhao, Jizong
author_facet Wang, Chengjun
Zhao, Meng
Wang, Jia
Zhang, Dong
Wang, Shuo
Zhao, Jizong
author_sort Wang, Chengjun
collection PubMed
description Moyamoya disease (MMD) is a rare chronic cerebrovascular disease mainly found in individuals of East Asian ethnicity, and its pathogenesis is largely unknown. Transfer RNA-derived fragments (tRFs) are novel biological entities involved in many biological processes; however, whether tRFs contribute towards MMD pathogenesis remains unexplored. In the present study, deep sequencing technology was used to identify alterations in tRF expression profiles between patients with MMD and healthy controls. The sequencing findings were validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Subsequently, the putative target genes of tRFs were predicted using miRNA target prediction algorithms. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to further evaluate potential functions of tRFs. The sequencing results demonstrated that 38 tRFs were differentially expressed between patients and controls, of which 22 were upregulated and 16 were downregulated. RT-qPCR analysis confirmed the validity of the sequencing results. GO and KEGG pathway enrichment analyses indicated that 15 pathways were associated with the selected tRFs. These pathways were mainly involved in angiogenesis and metabolism, both of which are physiopathological fundamentals of MMD. The results provided a novel insight into the mechanisms underlying MMD pathogenesis, and demonstrated that tRFs may serve as potential therapeutic targets for the future treatment of MMD.
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spelling pubmed-64721222019-04-23 Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study Wang, Chengjun Zhao, Meng Wang, Jia Zhang, Dong Wang, Shuo Zhao, Jizong Mol Med Rep Articles Moyamoya disease (MMD) is a rare chronic cerebrovascular disease mainly found in individuals of East Asian ethnicity, and its pathogenesis is largely unknown. Transfer RNA-derived fragments (tRFs) are novel biological entities involved in many biological processes; however, whether tRFs contribute towards MMD pathogenesis remains unexplored. In the present study, deep sequencing technology was used to identify alterations in tRF expression profiles between patients with MMD and healthy controls. The sequencing findings were validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Subsequently, the putative target genes of tRFs were predicted using miRNA target prediction algorithms. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to further evaluate potential functions of tRFs. The sequencing results demonstrated that 38 tRFs were differentially expressed between patients and controls, of which 22 were upregulated and 16 were downregulated. RT-qPCR analysis confirmed the validity of the sequencing results. GO and KEGG pathway enrichment analyses indicated that 15 pathways were associated with the selected tRFs. These pathways were mainly involved in angiogenesis and metabolism, both of which are physiopathological fundamentals of MMD. The results provided a novel insight into the mechanisms underlying MMD pathogenesis, and demonstrated that tRFs may serve as potential therapeutic targets for the future treatment of MMD. D.A. Spandidos 2019-05 2019-03-14 /pmc/articles/PMC6472122/ /pubmed/30896793 http://dx.doi.org/10.3892/mmr.2019.10024 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Chengjun
Zhao, Meng
Wang, Jia
Zhang, Dong
Wang, Shuo
Zhao, Jizong
Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study
title Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study
title_full Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study
title_fullStr Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study
title_full_unstemmed Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study
title_short Expression analysis of transfer RNA-derived fragments in the blood of patients with moyamoya disease: A preliminary study
title_sort expression analysis of transfer rna-derived fragments in the blood of patients with moyamoya disease: a preliminary study
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472122/
https://www.ncbi.nlm.nih.gov/pubmed/30896793
http://dx.doi.org/10.3892/mmr.2019.10024
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