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Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.

Background: Griffonia simplicifolia Baill. (Caesalpiniaceae) is a medicinal plant whose seeds are widely used in traditional medicine for their high content of 5-hydroxy-l-tryptophan (5-HTP), a direct precursor and enhancer of the activity of the brain hormone serotonin (5-HT). The plant extracts ar...

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Autores principales: Vigliante, Ivano, Mannino, Giuseppe, Maffei, Massimo E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472197/
https://www.ncbi.nlm.nih.gov/pubmed/30875930
http://dx.doi.org/10.3390/molecules24061032
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author Vigliante, Ivano
Mannino, Giuseppe
Maffei, Massimo E.
author_facet Vigliante, Ivano
Mannino, Giuseppe
Maffei, Massimo E.
author_sort Vigliante, Ivano
collection PubMed
description Background: Griffonia simplicifolia Baill. (Caesalpiniaceae) is a medicinal plant whose seeds are widely used in traditional medicine for their high content of 5-hydroxy-l-tryptophan (5-HTP), a direct precursor and enhancer of the activity of the brain hormone serotonin (5-HT). The plant extracts are used in dietary supplements aimed to alleviate serotonin-related disorders. Methods: In order to characterize the chemical components of G. simplicifolia seeds and their identity, we used a combined methodology by using HPLC-DAD-ESI-MS/MS for the qualitative and quantitative determination of the N-containing compounds, GC-FID and GC-MS for the characterization of the major fatty acids, and DNA fingerprinting based on PCR–RFLP for the unequivocal identification of the plant. Results: 5-HTP was the most representative compound, followed by lower percentages of the β-carboline alkaloid derivative griffonine and other alkaloids. Fatty acids were dominated by the unsaturated fatty acids linoleic acid and oleic acid, followed by the saturated fatty acids stearic and palmitic acids. PCR analysis of the internal transcribed spacer amplified sequence showed a major band at about 758 bp, whereas the PCR–RFLP analysis of this sequence using three different restriction enzymes (MspI, HhaI, and HaeIII) generated a specific fingerprinting useful for the plant identification. Conclusions: The combined chemical and molecular analysis of G. simplicifolia provided an interesting integrated approach for the unequivocal identification of commercial G. simplicifolia seeds.
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spelling pubmed-64721972019-04-26 Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill. Vigliante, Ivano Mannino, Giuseppe Maffei, Massimo E. Molecules Article Background: Griffonia simplicifolia Baill. (Caesalpiniaceae) is a medicinal plant whose seeds are widely used in traditional medicine for their high content of 5-hydroxy-l-tryptophan (5-HTP), a direct precursor and enhancer of the activity of the brain hormone serotonin (5-HT). The plant extracts are used in dietary supplements aimed to alleviate serotonin-related disorders. Methods: In order to characterize the chemical components of G. simplicifolia seeds and their identity, we used a combined methodology by using HPLC-DAD-ESI-MS/MS for the qualitative and quantitative determination of the N-containing compounds, GC-FID and GC-MS for the characterization of the major fatty acids, and DNA fingerprinting based on PCR–RFLP for the unequivocal identification of the plant. Results: 5-HTP was the most representative compound, followed by lower percentages of the β-carboline alkaloid derivative griffonine and other alkaloids. Fatty acids were dominated by the unsaturated fatty acids linoleic acid and oleic acid, followed by the saturated fatty acids stearic and palmitic acids. PCR analysis of the internal transcribed spacer amplified sequence showed a major band at about 758 bp, whereas the PCR–RFLP analysis of this sequence using three different restriction enzymes (MspI, HhaI, and HaeIII) generated a specific fingerprinting useful for the plant identification. Conclusions: The combined chemical and molecular analysis of G. simplicifolia provided an interesting integrated approach for the unequivocal identification of commercial G. simplicifolia seeds. MDPI 2019-03-15 /pmc/articles/PMC6472197/ /pubmed/30875930 http://dx.doi.org/10.3390/molecules24061032 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vigliante, Ivano
Mannino, Giuseppe
Maffei, Massimo E.
Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
title Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
title_full Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
title_fullStr Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
title_full_unstemmed Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
title_short Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
title_sort chemical characterization and dna fingerprinting of griffonia simplicifolia baill.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472197/
https://www.ncbi.nlm.nih.gov/pubmed/30875930
http://dx.doi.org/10.3390/molecules24061032
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