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Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus
There is an increasing emergence of antibiotic-resistant Vibrio alginolyticus, a zoonotic pathogen that causes mass mortality in aquatic animals and infects humans; therefore, there is a demand for alternatives to antibiotics for the treatment and prevention of infections caused by this pathogen. On...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472347/ https://www.ncbi.nlm.nih.gov/pubmed/31000791 http://dx.doi.org/10.1038/s41598-019-42681-1 |
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author | Kim, Sang Guen Jun, Jin Woo Giri, Sib Sankar Yun, Saekil Kim, Hyoun Joong Kim, Sang Wha Kang, Jeong Woo Han, Se Jin Jeong, Dalsang Park, Se Chang |
author_facet | Kim, Sang Guen Jun, Jin Woo Giri, Sib Sankar Yun, Saekil Kim, Hyoun Joong Kim, Sang Wha Kang, Jeong Woo Han, Se Jin Jeong, Dalsang Park, Se Chang |
author_sort | Kim, Sang Guen |
collection | PubMed |
description | There is an increasing emergence of antibiotic-resistant Vibrio alginolyticus, a zoonotic pathogen that causes mass mortality in aquatic animals and infects humans; therefore, there is a demand for alternatives to antibiotics for the treatment and prevention of infections caused by this pathogen. One possibility is through the exploitation of bacteriophages. In the present study, the novel bacteriophage pVa-21 was classified as Myoviridae and characterised as a candidate biocontrol agent against V. alginolyticus. Its morphology, host range and infectivity, growth characteristics, planktonic or biofilm lytic activity, stability under various conditions, and genome were investigated. Its latent period and burst size were estimated to be approximately 70 min and 58 plaque-forming units/cell, respectively. In addition, phage pVa-21 can inhibit bacterial growth in both the planktonic and biofilm states. Furthermore, phylogenetic and genome analysis revealed that the phage is closely related to the giant phiKZ-like phages and can be classified as a new member of the phiKZ-like bacteriophages that infect bacteria belonging to the family Vibrionaceae. |
format | Online Article Text |
id | pubmed-6472347 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64723472019-04-25 Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus Kim, Sang Guen Jun, Jin Woo Giri, Sib Sankar Yun, Saekil Kim, Hyoun Joong Kim, Sang Wha Kang, Jeong Woo Han, Se Jin Jeong, Dalsang Park, Se Chang Sci Rep Article There is an increasing emergence of antibiotic-resistant Vibrio alginolyticus, a zoonotic pathogen that causes mass mortality in aquatic animals and infects humans; therefore, there is a demand for alternatives to antibiotics for the treatment and prevention of infections caused by this pathogen. One possibility is through the exploitation of bacteriophages. In the present study, the novel bacteriophage pVa-21 was classified as Myoviridae and characterised as a candidate biocontrol agent against V. alginolyticus. Its morphology, host range and infectivity, growth characteristics, planktonic or biofilm lytic activity, stability under various conditions, and genome were investigated. Its latent period and burst size were estimated to be approximately 70 min and 58 plaque-forming units/cell, respectively. In addition, phage pVa-21 can inhibit bacterial growth in both the planktonic and biofilm states. Furthermore, phylogenetic and genome analysis revealed that the phage is closely related to the giant phiKZ-like phages and can be classified as a new member of the phiKZ-like bacteriophages that infect bacteria belonging to the family Vibrionaceae. Nature Publishing Group UK 2019-04-18 /pmc/articles/PMC6472347/ /pubmed/31000791 http://dx.doi.org/10.1038/s41598-019-42681-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kim, Sang Guen Jun, Jin Woo Giri, Sib Sankar Yun, Saekil Kim, Hyoun Joong Kim, Sang Wha Kang, Jeong Woo Han, Se Jin Jeong, Dalsang Park, Se Chang Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus |
title | Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus |
title_full | Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus |
title_fullStr | Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus |
title_full_unstemmed | Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus |
title_short | Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus |
title_sort | isolation and characterisation of pva-21, a giant bacteriophage with anti-biofilm potential against vibrio alginolyticus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472347/ https://www.ncbi.nlm.nih.gov/pubmed/31000791 http://dx.doi.org/10.1038/s41598-019-42681-1 |
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