Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves()

The localization of Cassava brown streak virus (CBSV) in cassava (Manihot esculenta) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain r...

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Autores principales: Saggaf, Maliha H., Ndunguru, Joseph, Tairo, Fred, Sseruwagi, Peter, Ascencio-Ibáñez, José Trino, Kilalo, Dora, Miano, Douglas W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472608/
https://www.ncbi.nlm.nih.gov/pubmed/31007375
http://dx.doi.org/10.1016/j.pmpp.2018.06.001
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author Saggaf, Maliha H.
Ndunguru, Joseph
Tairo, Fred
Sseruwagi, Peter
Ascencio-Ibáñez, José Trino
Kilalo, Dora
Miano, Douglas W.
author_facet Saggaf, Maliha H.
Ndunguru, Joseph
Tairo, Fred
Sseruwagi, Peter
Ascencio-Ibáñez, José Trino
Kilalo, Dora
Miano, Douglas W.
author_sort Saggaf, Maliha H.
collection PubMed
description The localization of Cassava brown streak virus (CBSV) in cassava (Manihot esculenta) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed precipitation in CBSV-infected but not CBSV-free tissues, demonstrating successful localization of CBSV. Microscopic inspection showed significantly larger (P < 0.001) midribs in CBSV-infected compared with control (uninfected) leaves. Viral accumulation occurred in middle and lower but rarely in young upper leaves. This immunohistochemical method for virus localization will be invaluable for efficient screening of CBSV and for breeding resistant cassava.
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spelling pubmed-64726082019-04-19 Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves() Saggaf, Maliha H. Ndunguru, Joseph Tairo, Fred Sseruwagi, Peter Ascencio-Ibáñez, José Trino Kilalo, Dora Miano, Douglas W. Physiol Mol Plant Pathol Article The localization of Cassava brown streak virus (CBSV) in cassava (Manihot esculenta) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed precipitation in CBSV-infected but not CBSV-free tissues, demonstrating successful localization of CBSV. Microscopic inspection showed significantly larger (P < 0.001) midribs in CBSV-infected compared with control (uninfected) leaves. Viral accumulation occurred in middle and lower but rarely in young upper leaves. This immunohistochemical method for virus localization will be invaluable for efficient screening of CBSV and for breeding resistant cassava. Academic Press 2019-01 /pmc/articles/PMC6472608/ /pubmed/31007375 http://dx.doi.org/10.1016/j.pmpp.2018.06.001 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saggaf, Maliha H.
Ndunguru, Joseph
Tairo, Fred
Sseruwagi, Peter
Ascencio-Ibáñez, José Trino
Kilalo, Dora
Miano, Douglas W.
Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves()
title Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves()
title_full Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves()
title_fullStr Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves()
title_full_unstemmed Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves()
title_short Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves()
title_sort immunohistochemical localization of cassava brown streak virus and its morphological effect on cassava leaves()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472608/
https://www.ncbi.nlm.nih.gov/pubmed/31007375
http://dx.doi.org/10.1016/j.pmpp.2018.06.001
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