Cargando…

Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin

[Image: see text] Opioid peptides are critically involved in a variety of physiological functions necessary for adaptation and survival, and as such, understanding the precise actions of endogenous opioid peptides will aid in identification of potential therapeutic strategies to treat a variety of d...

Descripción completa

Detalles Bibliográficos
Autores principales: Calhoun, S. E., Meunier, C. J., Lee, C. A., McCarty, G. S., Sombers, L. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473485/
https://www.ncbi.nlm.nih.gov/pubmed/30571911
http://dx.doi.org/10.1021/acschemneuro.8b00351
_version_ 1783412441288278016
author Calhoun, S. E.
Meunier, C. J.
Lee, C. A.
McCarty, G. S.
Sombers, L. A.
author_facet Calhoun, S. E.
Meunier, C. J.
Lee, C. A.
McCarty, G. S.
Sombers, L. A.
author_sort Calhoun, S. E.
collection PubMed
description [Image: see text] Opioid peptides are critically involved in a variety of physiological functions necessary for adaptation and survival, and as such, understanding the precise actions of endogenous opioid peptides will aid in identification of potential therapeutic strategies to treat a variety of disorders. However, few analytical tools are currently available that offer both the sensitivity and spatial resolution required to monitor peptidergic concentration fluctuations in situ on a time scale commensurate with that of neuronal communication. Our group has developed a multiple-scan-rate waveform to enable real-time voltammetric detection of tyrosine containing neuropeptides. Herein, we have evaluated the waveform parameters to increase sensitivity to methionine-enkephalin (M-ENK), an endogenous opioid neuropeptide implicated in pain, stress, and reward circuits. M-ENK dynamics were monitored in adrenal gland tissue, as well as in the dorsal striatum of anesthetized and freely behaving animals. The data reveal cofluctuations of catecholamine and M-ENK in both locations and provide measurements of M-ENK dynamics in the brain with subsecond temporal resolution. Importantly, this work also demonstrates how voltammetric waveforms can be customized to enhance detection of specific target analytes, broadly speaking.
format Online
Article
Text
id pubmed-6473485
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-64734852019-04-23 Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin Calhoun, S. E. Meunier, C. J. Lee, C. A. McCarty, G. S. Sombers, L. A. ACS Chem Neurosci [Image: see text] Opioid peptides are critically involved in a variety of physiological functions necessary for adaptation and survival, and as such, understanding the precise actions of endogenous opioid peptides will aid in identification of potential therapeutic strategies to treat a variety of disorders. However, few analytical tools are currently available that offer both the sensitivity and spatial resolution required to monitor peptidergic concentration fluctuations in situ on a time scale commensurate with that of neuronal communication. Our group has developed a multiple-scan-rate waveform to enable real-time voltammetric detection of tyrosine containing neuropeptides. Herein, we have evaluated the waveform parameters to increase sensitivity to methionine-enkephalin (M-ENK), an endogenous opioid neuropeptide implicated in pain, stress, and reward circuits. M-ENK dynamics were monitored in adrenal gland tissue, as well as in the dorsal striatum of anesthetized and freely behaving animals. The data reveal cofluctuations of catecholamine and M-ENK in both locations and provide measurements of M-ENK dynamics in the brain with subsecond temporal resolution. Importantly, this work also demonstrates how voltammetric waveforms can be customized to enhance detection of specific target analytes, broadly speaking. American Chemical Society 2018-12-20 /pmc/articles/PMC6473485/ /pubmed/30571911 http://dx.doi.org/10.1021/acschemneuro.8b00351 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Calhoun, S. E.
Meunier, C. J.
Lee, C. A.
McCarty, G. S.
Sombers, L. A.
Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin
title Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin
title_full Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin
title_fullStr Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin
title_full_unstemmed Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin
title_short Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin
title_sort characterization of a multiple-scan-rate voltammetric waveform for real-time detection of met-enkephalin
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473485/
https://www.ncbi.nlm.nih.gov/pubmed/30571911
http://dx.doi.org/10.1021/acschemneuro.8b00351
work_keys_str_mv AT calhounse characterizationofamultiplescanratevoltammetricwaveformforrealtimedetectionofmetenkephalin
AT meuniercj characterizationofamultiplescanratevoltammetricwaveformforrealtimedetectionofmetenkephalin
AT leeca characterizationofamultiplescanratevoltammetricwaveformforrealtimedetectionofmetenkephalin
AT mccartygs characterizationofamultiplescanratevoltammetricwaveformforrealtimedetectionofmetenkephalin
AT sombersla characterizationofamultiplescanratevoltammetricwaveformforrealtimedetectionofmetenkephalin